Method for promoting quick proliferation of Botryococcus braunii
A grape algae, fast technology, applied in the field of microorganisms, can solve the problems of long doubling time, shortening the generation time of grape algae, slow growth of grape algae, etc., and achieve the effect of increasing yield, large biomass, and promoting rapid proliferation
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example 1
[0032] Example 1 Effect of V elements on the growth of Botrytis 357
[0033] With the ability to provide light and CO for the growth of microalgae 2 The airlift photobioreactor was used as a container for grape algae cultivation. The effective volume of the reactor is 8.5L. The culture temperature is 25±2 ℃; fluorescent lamps are used as the light source, and the light intensity is about 120 μmol m -2 s -1 , continuous light; air flow rate of 0.85 L / min, CO 2 The flow rate is 0.15 L / min. The concentration of cells in each sample (OD 680 ), to characterize the cell growth of Botrytis in culture medium.
[0034] Store Botrytis 357 in a 250 mL glass Erlenmeyer flask as a culture algae, and culture it on a shaker until the cell OD 680 about 1.0. Microalgae were inoculated into the above-mentioned airlift photoreactor containing BGII-containing medium.
[0035] Dissolve ammonium vanadate into the prepared BGⅡ medium, so that the final concentration of elemental vanadium in...
example 2
[0040] Example 2 The Effect of W Elements on the Growth of Botrytis 357
[0041] Dissolve sodium tungstate into the prepared BGⅡ medium, so that the final concentration of element tungsten in the medium is 0 mg / mL, 10 mg / mL, respectively. -5 mg / mL, 10 -4 mg / mL, 10 -3 mg / mL, 10 -2 mg / mL, five experiments were carried out.
[0042] The rest are the same as Example 1.
[0043] The growth speed and concentration, dry cell weight of botrytis in the culture solution containing different concentrations of tungsten, and the comparison with the culture medium BGⅡ without adding the trace element of the present invention are as follows: image 3 , 4 shown.
[0044] Depend on image 3 , 4 It can be seen that when the concentration of sodium tungstate in the medium is lower than 10 -3 mg / mL, the concentration of botrytis algae cells increased. When the concentration of W element in the medium is 10 -4 mg / mL, the dry weight of algal cells is 2.2 g / L.
example 3
[0045] Example 3 Effect of Ce element on the growth of Botrytis 357
[0046] Dissolve cerium nitrate into the prepared BGⅡ medium so that the final concentrations of elemental cerium in the medium are 0 mg / mL, 10 mg / mL, respectively. -5 mg / mL, 10 -4 mg / mL, 10 -3 mg / mL, 10 -2 mg / mL, five experiments were carried out.
[0047] The rest are the same as Example 1.
[0048] The speed and concentration, dry cell weight of botrytis growing in the culture solution containing different concentrations of cerium, and the comparison with the medium BGⅡ without adding the trace elements of the present invention are as follows: Figure 5 , 6 shown.
[0049] Depend on Figure 5 , 6 It can be seen that when cerium nitrate is added to the medium, the growth of botrytis can be promoted. The optimum concentration for botrytis growth is 10 -5 mg / mL. At this time, the dry weight of algae cells can reach 2.4 g / L.
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