Method for extracting bio-oil by recycling botryococcus braunii cells
A technology of Botrytis brannica and biomass oil, which is applied in the field of reusing Botrytis brannicola cells to extract biomass oil, which can solve the problems of strong light, slow growth, and low culture density, so as to improve the production intensity of oil and reduce the processing cost , the effect of rapid oil accumulation
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Embodiment 1
[0028] Collect the wet algae with a certain volume of Botrytis brachyphyllum culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 90%; put the wet algae into a stirring reactor, add n-hexane at room temperature An extraction solvent composed of alkane and ethylene glycol dimethyl ether (100:2, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 6 times the volume of the wet algae, and stirred at a speed of 400rpm for 10min , separate the algae cells and collect the extraction solvent containing oil, add new extraction solvent again, repeat the extraction operation steps, the wet algae body is extracted 3 times in total, and the total oil extraction rate is 30%; Solvents are combined, vacuum rotary evaporation is carried out, the extraction solvent is recovered for reuse, and the obtained biomass oil is collected; the wet algae cells extracted from the biomass oil are added...
Embodiment 2
[0030]Collect the wet algae with a certain volume of Botrytis brachyphylla culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 95%; put the wet algae into a stirring reactor, add the An extraction solvent composed of alkane and ethylene glycol dimethyl ether (100:1, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 4 times the volume of the wet algae body, and stirred at a speed of 200rpm for 15min , separate the algae cells and collect the extraction solvent containing oil, add new extraction solvent again, repeat the extraction operation steps, the wet algae body is extracted twice in total, and the total oil extraction rate is 45%; Solvents are combined, vacuum rotary evaporation is carried out, the extraction solvent is recovered for reuse, and the obtained biomass oil is collected; the wet algae cells extracted from the biomass oil are added to a fresh medium 25 ti...
Embodiment 3
[0032] Collect the wet algae with a certain volume of Botrytis brachyphyllum culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 92%; put the wet algae into a stirring reactor, add the An extraction solvent composed of alkanes and ethylene glycol dimethyl ether (100: 1.5, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 5 times the volume of the wet algae, and stirred at a speed of 300rpm for 12min , separate the algae cells and collect the extraction solvent containing biomass oil, re-add new extraction solvent, repeat the extraction operation steps, the wet algae body is extracted 3 times in total, and the total oil extraction rate is 50%; the collected biomass oil containing The extraction solvents were combined, vacuum rotary evaporation was carried out, the extraction solvent was recovered for reuse, and the obtained biomass oil was collected; the wet algae cells ...
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