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Cottonseed protein for fermentation and preparation method and application thereof

A cottonseed protein and cottonseed technology, applied in the field of fermented cottonseed protein and its preparation, can solve the problems of shortening of rapid utilization, no improvement of lag period and the like

Inactive Publication Date: 2013-07-24
中棉紫光生物科技(上海)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this technology can remove a small amount of unremoved shells, velvet, starch, fibers, etc. contained in cottonseed protein and increase the protein content, it has no improvement in promoting the rapid utilization of bacteria and shortening the lag period.

Method used

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  • Cottonseed protein for fermentation and preparation method and application thereof
  • Cottonseed protein for fermentation and preparation method and application thereof
  • Cottonseed protein for fermentation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Adopt conventional methods in this field to carry out the treatment of cottonseed delinting, shelling, cotton kernel softening, embryo pressing, drying, oil extraction, phenol removal, and partial desolvation, after obtaining wet cotton meal, such as figure 1 Shown is prepared according to the following process and conditions:

[0026] Step 1. The wet cotton meal is transported to the dryer at a uniform speed by the feeder. The wet meal passes through the sprayer before entering the dryer. During the forward process of the wet cotton meal, the nutrient solution is evenly distributed by the sprayer. Spray onto wet cottonseed meal.

[0027] Preparation of nutrient solution: 3.75g / 100mL CuSO 4 ·5H 2 O, 7g / 100mL MgSO 4 ·7H 2 O, 0.6g / 100mL CoCl 2 ·7H 2 O, 10g / 100mL betaine, 20g / 100mL enzymatic cottonseed peptone, 0.05g / 100mL Triton X100. The amount of nutrient solution added was 10 mL of nutrient solution per kilogram of cottonseed meal.

[0028] Step 2. After the we...

Embodiment 2

[0031] The nutrient solution adopted in the present embodiment is: 1.5g / 100mL CuSO 4 ·5H 2 O, 10.0g / 100mL MgSO 4 ·7H 2 O, 0.1g / 100mL CoCl 2 ·7H 2 O, 5g / 100mL betaine, 40g / 100mL enzymatic cottonseed peptone, 0.01g / 100mL Triton X100. The amount of nutrient solution added was 10 mL of nutrient solution per kilogram of cottonseed meal. Other steps and conditions are with embodiment 1.

Embodiment 3

[0033] 1. Product processing:

[0034]The nutrient solution adopted in the present embodiment is: 7.5g / 100mL CuSO 4 ·5H 2 O, 2.5g / 100mL MgSO 4 ·7H 2 O, 0.8g / 100mL CoCl 2 ?7H 2 O, 10g / 100mL betaine, 5g / 100mL enzymatic cottonseed peptone, 0.05g / 100mL Triton X100. The amount of nutrient solution added was 10 mL of nutrient solution per kilogram of cottonseed meal. Other steps and conditions are with embodiment 1.

[0035] Two, the protein and gossypol content detection index of embodiment 1-3

[0036] The protein and gossypol content values ​​of cottonseed protein are shown in Table 1:

[0037]

[0038] Three, embodiment 1 improves the effect detection of erythromycin output

[0039] Taking Example 1 and general cottonseed protein as the nitrogen source tested, and taking the most commonly used soybean meal in erythromycin fermentation as a control, the effect of Example 1 on the increase of erythromycin fermentation titer was investigated.

[0040] The detection met...

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Abstract

The invention discloses a cottonseed protein for fermentation and a preparation method and application thereof. A nutrient solution is sprayed to wet cottonseed meal, and then the cottonseed protein is obtained by drying and velvet removal, wherein every 100mL of nutrient solution contains 1.0-5.0g of enzymolysis cottonseed peptone, 1.0-40g of betaine, 1.0-10g of CuSO4.5H2O, 1.0-10g of MgSO4.7H2O, 0.1-1.0g of CoCl2.7H2O and 0.01-0.5g of Triton X100. According to the invention, thallus reproduction is promoted while maintaining the advantages of the fermentation technology of the existing cottonseed protein product, the cell growth rate is improved, the biomass of the growing period is increased, and the growing period is shortened; and meanwhile, the release of metabolite from the cell inside to outside can be promoted so as to increase the output of target products and even improve the product quality or shorten the fermentation period.

Description

technical field [0001] The invention relates to a method in the technical field of fermentation, in particular to a cottonseed protein used for fermentation and its preparation method and application. Background technique [0002] Fermentation technology is an important production technology in the production or processing of biopharmaceuticals, chemical products, food and beverages. The fermentation process includes two stages of bacterial growth and product formation. For the fermentation production process of microbial drugs with secondary metabolites as products, the two stages of bacterial growth and product formation are often uncoupled. The early stage of fermentation is the growth stage. This stage is aimed at rapidly multiplying the bacteria. When the cell reproduction reaches a certain number, the substrate consumption and the metabolites reach a certain level, the fermentation process gradually migrates to the production stage that is dominated by product formatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12P19/62C12P21/06C12R1/01
Inventor 王勇郝玉有顾东王立琴
Owner 中棉紫光生物科技(上海)有限公司
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