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Method for culturing red algae with high-yield phycocyanin

A cultivation method and phycocyanin technology, applied in the field of efficient red algae cultivation, can solve the problems of unregulated, long doubling time, low efficiency, etc., and achieve the reduction of the number of devices and equipment, high metabolite yield, and improved cultivation. effect of speed

Active Publication Date: 2016-12-07
云南保山泽元藻业健康科技有限公司
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Problems solved by technology

Although the heterotrophic culture process of red algae can achieve rapid growth of biomass, the content of phycocyanin in the obtained algal cells is low, so it cannot be used for large-scale cultivation of red algae for the purpose of high-yield phycocyanin
In addition, in the heterotrophic process, the medium usually selected is a common microalgae heterotrophic medium, without adding plant growth hormone substances that promote phycocyanin accumulation, and the culture process is usually not regulated or unoptimized by intermittent feeding. Feeding medium, this method does not consider the difference in nutritional requirements between red algae heterotrophs and ordinary photoautotrophs, resulting in poor accumulation of phycocyanin in the medium and low phycocyanin yield
[0007] On the other hand, photoautotrophy can obtain algae cells with higher phycocyanin content. At present, the algae species used in the cultivation of red algae are obtained by photoautotrophy; at the same time, accumulating phycocyanin in cells is an extremely consuming energy process, the efficiency is low, which means that the photoautotrophic growth rate and yield of red algae are lower than other microalgae, and the doubling time is longer than other microalgae

Method used

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  • Method for culturing red algae with high-yield phycocyanin
  • Method for culturing red algae with high-yield phycocyanin
  • Method for culturing red algae with high-yield phycocyanin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0170] Example 1: Research on algae cell growth during heterotrophic and photoautotrophic culture of red algae

[0171] This example is used to compare the culture results of heterotrophic, polytrophic and photoautotrophic red algal cells. The red algae cells used in this example are red algae Galdieria sulphuraria 074G.

[0172] Heterotrophic and polyculture shake flask medium composition: (NH 4 ) 2 SO 4 5 g / L, Glucose 35 g / L, KH 2 PO 4 0.2 g / L, MgSO 4 ·7H 2 O 0.3 g / L, CaCl 2 2H 2 O 0.01 g / L, NaCl 0.01 g / L, Fe-EDTA 0.1 g / L, abscisic acid 0.1 mg / L, gibberellin 0.1 mg / L, brassin 1 mg / L, trace elements 2ml and water, of which The composition of trace elements is H 3 BO3 5 mg / L, MnCl 2 4H 2 O 5 mg / L, ZnSO 4 ·7H 2 O 1 mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 1 mg / L, CuSO 4 ·5H 2 O 0.5 mg / L, NaVO 3 4H 2 O 0.1 mg / L, CoCl 2 ·6H 2 O 0.1 mg / l.

[0173] Photoautotrophic shake flask medium composition: (NH 4 ) 2 SO 4 5 g / L, KH 2 PO 4 0.2 g / L, MgSO 4 ·7H 2 O...

Embodiment 2

[0177] Embodiment 2: Red algae is compared in the heterotrophic culture process in the bioreactor of different levels

[0178] This example is used to compare the results of heterotrophic culture of red algae cells in different levels of bioreactors. The bioreactors in this example are 1L and 5L shake flasks, and the red algae cells used in this example are red algae Galdieriasulphuraria 074G.

[0179] The inoculum density of red algae 1L and 5L shake flask heterotrophic culture is 0.52g / l, the temperature is 40°C, and the rotation speed is 150rmp. After 8 days of heterotrophic culture of red algae, the glucose in the culture medium was exhausted, and the final algal cell densities of 1L and 5L shake flasks were 8.23g / l and 8.40g / L, respectively, which could be used as seeds for the next photoautotrophic culture. The results are shown in figure 2 , the standard deviation in the figure is the result of 3 independent separate experiments.

[0180] Add the heterotrophic med...

Embodiment 3

[0183] Embodiment 3: red algae heterotrophic seeds and photoautotrophic seeds are carried out photobioreactor in indoor 1L glass column photobioreactor Research on Autotrophic Culture

[0184] This example was carried out in an indoor 1L cylindrical airlift photobioreactor, and algae cells obtained under heterotrophic and photoautotrophic culture conditions were used as seeds respectively, wherein the heterotrophic algal cells were the cells obtained in Example 2. In this example, the dry weight and phycocyanin content of heterotrophic and photoautotrophic seeds were measured respectively during the photoautotrophic process.

[0185] The inoculation density of heterotrophic and photoautotrophic seeds is 0.5g / l, the temperature of photoautotrophic culture is 40℃, and 5% CO 2 , The ventilation volume is 0.25vvm. In photoautotrophic culture, the light intensity was 250 μmolm -2 the s -1 , continuous light. After photoautotrophic culture to 8 days, the dry weight of hetero...

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Abstract

The invention relates to a method for culturing red algae with high-yield phycocyanin. The method comprises the steps of red algae heterotrophy or polyculture and photoautotrophic cultivation. The photoautotrophic cultivation is performed by taking algae cells obtained by heterotrophism or polyculture as seeds. The invention further relates to a method for quickly accumulating phycocyanin. By adoption of the method, the advantage that the algae cells grow quickly when the red algae are at a heterotrophy or polyculture stage, and plenty of algae seeds are provided for a follow-up photoautotrophic stage. The red algae grow quickly at the photoautotrophic stage, and phycocyanin is accumulated. According to the method, the problems that in the existing photoautotrophic cultivation process of the red algae, the cell growth rate is too low and the content and yield are low when microalgae are utilized to produce phycocyanin can be solved.

Description

technical field [0001] The present invention relates to a high-efficiency method for cultivating red algae. The method includes heterotrophic or polytrophic culture and photoautotrophic culture of red algae. The photoautotrophic culture uses algae cells obtained from heterotrophic or polytrophic culture as seeds to produce implement. The present invention also relates to a method for rapidly accumulating phycocyanin. Background technique [0002] Red algae belong to Rhodophyta. The algal body contains chlorophyll a, chlorophyll b, lutein and carotene, as well as a large amount of phycoerythrin and phycocyanin. Often due to the different content of various pigments, the algal body appears bright red or Pink, purple, fuchsia and other different colors. Some unicellular red algae (unicellular rhodophyte) can accumulate a large amount of special pigments——phycocyanin (Graverholt O and Eriksen N., Heterotrophic high-cell-density fed-batch and continuous-flow cultures of Galdier...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P21/02C12R1/89
CPCC07K14/795C12N1/12C12P21/02
Inventor 李元广章真万民熙王振旸范建华黄建科王骏王军王伟良俞安全
Owner 云南保山泽元藻业健康科技有限公司
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