Method for introducing frame-shift mutation in MSTN (myostatin) genes of cattle
A frame-shift mutation and genetic technology, applied in the field of genetic engineering, can solve the problems of inability to perform biological functions, etc., and achieve the effects of abnormal muscle groups, high lean meat percentage, and thin skin
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[0031] The present invention will be further described below in conjunction with specific embodiments, and its specific embodiments should be understood as illustrations only, not limiting, and the protection scope of the present invention cannot be limited by the following examples.
[0032] Design idea of the present invention is:
[0033] According to the principle of applying the Cre-LoxP system to introduce frameshift mutations into the genome, two homology arms were designed, and the exon carrying the frameshift mutation was located on the short homology arm near the end of the screening gene. In order to keep the genome as unaffected as possible before and after targeting, the insertion point is selected in the intron, so that the inserted LoxP and other redundant sequences can be located in the intron. The frameshift mutation should be placed as close to the positive screening marker gene as possible. The frameshift mutation is located at the third exon of the bovine...
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