32 results about "Very long chain" patented technology

Oilseed plants which have been transformed to produce at least 8.0% arachidonic acid (ARA) as well as uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

Oilseed plants which have been transformed to produce very long chain polyunsaturated fatty acids, recombinant constructs used in such transformations, methods for producing such fatty acids in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

The invention provides methods of synthesizing omega-3 and omega-6 very long chain polyunsaturated fatty acids (VLC-PUFAs, C28-C42:4, 5 and 6), analogs and derivatives thereof, pharmaceutical compositions containing these isolated VLC-PUFA compounds and therapeutic uses therefor.

The invention relates to a reinforced and toughened anti-bacterial polypropylene composition and a preparation method thereof. The composition comprises the following components in weight percent: 54% to 87.7% of polypropylene, 0.5% to 5% of a coupling-modified inorganic antimicrobial agent, 10% to 30% of ultra-high molecular weight polyethylene master batch, 1% to 8% of graft polypropylene; 0.2% to 1% of a stabilizing agent, 0.2% to 1% of a dispersing agent and 0.4% to 1% of anti-oxidant. According to the invention, ultra-high molecular weight polyethylene is prepared into ultra-high molecular weight polyethylene master batch under certain conditions, and the ultra-high molecular weight polyethylene master batch, the surface-modified inorganic antimicrobial agent, the polypropylene, the graft polypropylene and other additives are quickly mixed and extruded to be manufactured into a polypropylene composite material with high strength, high ductility and high mold resistance. Under the condition that the inorganic antimicrobial agent is added to endow excellent antibacterial activity to the polypropylene, organic very long-chain high-polymer reinforced and toughened polypropylene is added, so that the prepared polypropylene composite material not only has excellent mold resistance but also has outstanding mechanical characteristics.

The present invention relates to processes for production of Very Long Chain Polyunsaturated Fatty Acids (VLC-PUFAs). The present invention also relates to compositions (e.g., nutritional supplements and food products) containing such VLC-PUFAs. In one embodiment, the present invention is directed to methods for biosynthesis and production of the VLC-PUFAs described herein (particularly C28-C38 PUFAs, also referred to herein as supraenes or supraenoics) by the expression, in a production host cell, of the full or partial sequence(s) of Elovl4 DNA / mRNA nucleic acids or ELOVL4 protein sequences encoded thereby, from any species (prokaryotic or eukaryotic) for use in the biosynthesis, production, purification and utilization of VLC-PUFAs in particular by the elongation of C18-C26 saturated fatty acids and PUFAs. The composition of the invention comprises, in various embodiments, a dietary supplement, a food product, a pharmaceutical formulation, a humanized animal milk, an infant formula, a cosmetic item and a biodiesel fuel for example. A pharmaceutical formulation can include, but is not limited to: a drug for treatment of neurodegenerative disease, a retinal disorder, age related maculopathy, a fertility disorder, particularly regarding sperm or testes, or a skin disorder.

The present invention relates to structured triglyceride, to parental nutrition emulsions comprising same, and use thereof. In particular, the invention relates to structured triglycerides comprising at least one medium chain C6-C12 fatty acid and at least one fatty acid selected from the group consisting of long chain C14-C18 or very long chain C20-C22 fatty acids, preferably each fatty acid is present in a predetermined position of the glycerol backbone. The parenteral nutrition emulsions are particularly useful for nourishing preterm-and term-infants, children, critically ill patients, and cancer patients.

This invention relates to seeds of plant, plants themselves and cells of such plants which comprise a heterologous gene coding for a plant (such as nasturtium (Tropaeolum majus) or Crambe abyssinica) fatty acid elongase (FAE) gene or allelic variant thereof, or combinations of one or both of these FAE genes with an Arabidopsis fatty acid elongase 1 (FAE1) gene, in co-transformation, in reading frame alignment with a promoter capable of increasing expression of said gene(s), when said transformed plant cell is in a seed, said plant cell or seed being capable of producing an increase in proportion of a very long chain monounsaturated or saturated fatty acids when compared with the proportions of said fatty acids in a control plant cell or seed lacking said heterologous FAE gene or genes. The invention also relates to combinations of these fatty acid elongase genes by traditional crossing, sufficient to increase the proportion of very long chain monounsaturated or saturated fatty acids in the seed oil of the progeny compared to the proportion of said fatty acids in either of the parental lines.

The present invention relates to herbicidally active compositions, which comprise 3-[5-(difluoromethoxy)-1-methyl-3-(trifluoromethyl)pyrazol-4-ylmethylsulfonyl]-4,5-dihydro-5,5-dimethyl-1,2-oxazole [common name pyroxasulfone] and at least one herbicide B and at least one herbicide compound B which is an selected from inhibitors of the very long chain fatty acids (VLCFA) synthesis.The invention furthermore relates to the use of a composition as defined herein for controlling undesirable vegetation, in particular in crops. When using the compositions of the invention for this purpose the herbicide A and the at least one herbicide B can be applied simultaneously or in succession, where undesirable vegetation may occur.

The invention provides a gas chromatography-mass spectrometry detection method of three very long chain fatty acids in a human serum. The method comprises the following steps (1) carrying out pretreatment on a human serum sample to obtain a derivative product; (2) detecting the derivative product by using a gas chromatography-mass spectrometer; (3) carrying out qualitative analysis on each very long chain fatty acid in the human serum sample according to a total ion chromatogram and a mass spectrogram of the derivative product, and calculating the content of each very long chain fatty acid in the human serum sample according to a selective ion chromatogram, wherein each very long chain fatty acid comprises docosanoic acid, tetracosanoic acid and hexacosanoic acid. Compared with the background art, the pretreatment process disclosed by the invention is fast, sensitive and accurate.

The present invention relates to processes for production of Very Long Chain Polyunsaturated Fatty Acids (VLC-PUFAs). The present invention also relates to compositions (e.g., nutritional supplements, food products, and pharmaceutic compositions) containing such VLC-PUFAs. In one embodiment, the present invention is directed to methods for biosynthesis and production of the VLC-PUFAs described herein (particularly C28-C40 PUFAs, also referred to herein as supraenes or supraenoics) by the expression, in a production host cell, of the full or partial sequence(s) of Elovl4 DNA / mRNA nucleic acids or ELOVL4 protein sequences encoded thereby, from any species (prokaryotic or eukaryotic) for use in the biosynthesis, production, purification and utilization of VLC-PUFAs in particular by the elongation of C18-C26 saturated fatty acids and PUFAs. The composition of the invention comprises, in various embodiments, a dietary supplement, a food product, a nutritional formulation, a pharmaceutical formulation, a humanized animal milk, an infant formula, and a cosmetic item and for example. A pharmaceutical formulation can include, but is not limited to: a composition for enhancing neural development and function, a drug for treatment of neurodegenerative disease, an ocular disorder, a retinal disorder, age related maculopathy, a fertility disorder, particularly regarding sperm or testes, or a skin disorder.

A cosmeceutically acceptable formulation as a wound dressing including red palm oil and a triglyceride, preferably fish oil, preferably derived from salmon. Another aspect of this disclosure is providing a mixture of red palm oil and very long chain (>C>18) omega 3 and 6 fatty acids. The formulation may also include one or more of the following: coconut oil and shea butter for anti-inflammatory activity and antimicrobial efficacy, fish collagen, an acidic preservative, a cationic surfactant, and various vitamins.

The invention provides a method and kit for determining very long chain fatty acids in a sample by liquid chromatography tandem mass spectrometry. The method comprises the steps that very long chain fatty acid standardized product solutions with different concentration are prepared; stable isotope internal standards are added to the standardized product solutions to be mixed uniformly, and acetonitrile and concentrated hydrochloric acid are added for sample pretreatment; then n-hexane is used for extraction, nitrogen is used for blowing dry supernatant, and liquid chromatography tandem mass spectrometry detection is performed after redissolution; a standard curve is drawn according to the concentration of the standardized product solutions and the detected signal intensity; the standardized product solutions in the step B are replaced with the sample to be detected for detection; according to a detection result of the sample to be tested and compared with the standard curve, the concentration of the very long chain fatty acids in the sample to be detected is obtained, and thus, the quantitative detection of the very long chain fatty acids in the sample is realized. The method for determining the very long chain fatty acids in the sample by liquid chromatography tandem mass spectrometry is successfully established, and the method can quickly and specifically detect the content of the very long chain fatty acids in samples (especially serum samples) with a small use amount of samples.

The invention discloses separated peanut beta-ketoacyl-CoA synthase (KCS) genes AhyA.KCS1 (SEQ ID No. 2) and AhyB.KCS1 (SEQ ID No. 4). The invention also provides a method for producing transgenic yeast and plants by using the genes; the method can increase the content of very long chain fatty acids, especially behenic acid, in the plants, and the highest content reaches 12.3%; the total amount ofVLCFA in the yeast is increased to 6.1% from 0.4%, namely, is increased by nearly 15 times; the total amount of the VLCFA in the arabidopsis transgenic plant is increased to 25.0% or above, namely, is increased by 4 times or more. The method has an important application value in the improvement of plant fatty acids and the cultivation and production of high value-added oil crops.

A method for determining colorectal cancer risk includes obtaining a blood sample of the subject, isolating serum or EDTA plasma from the blood sample, analyzing the serum or EDTA plasma to determine plasma levels of very long chain dicarboxylic acid (VLCDCA 28:4), comparing the determined plasmas level of VLCDCA 28:4 of the subject with a predetermined range of plasma levels of VLCDCA 28:4 of diagnosed subjects having colorectal cancer, and determining a colorectal cancer risk exists when the determined plasma level of VLCDCA 28:4 is within the predetermined range of plasma levels of VLCDCA.

The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

The present invention relates to processes for production of Very Long Chain Polyunsaturated Fatty Acids (VLC-PUFAs). The present invention also relates to compositions (e.g., nutritional supplements and food products) containing such VLC-PUFAs. In one embodiment, the present invention is directed to methods for biosynthesis and production of the VLC-PUFAs described herein (particularly C28-C38 PUFAs, also referred to herein as supraenes or supraenoics) by the expression, in a production host cell, of the full or partial sequence(s) of Elovl4 DNA / mRNA nucleic acids or ELOVL4 protein sequences encoded thereby, from any species (prokaryotic or eukaryotic) for use in the biosynthesis, production, purification and utilization of VLC-PUFAs in particular by the elongation of C18-C26 saturated fatty acids and PUFAs. The composition of the invention comprises, in various embodiments, a dietary supplement, a food product, a pharmaceutical formulation, a humanized animal milk, an infant formula, a cosmetic item and a biodiesel fuel for example. A pharmaceutical formulation can include, but is not limited to: a drug for treatment of neurodegenerative disease, a retinal disorder, age related maculopathy, a fertility disorder, particularly regarding sperm or testes, or a skin disorder.

This invention relates to nucleic acid sequences coding for a Lunaria annua, Cardamine graeca or Teesdalia nudicaulis fatty acid elongase, yeast cells expressing the genes / enzymes, plants themselves and cells of such plants and seeds which contain a heterologous gene coding for a L. annua, C. graeca or T. nudicaulis fatty acid elongase gene, the plant or seed being capable of producing increased proportion of a very long chain monounsaturated fatty acid, especially nervonic acid and eicosenoic acid, beyond that of a control plant or seed lacking the heterologous FAE gene or genes.

This disclosure provides compounds, pharmaceutical compositions, and methods of use for the prevention and treatment of inflammatory diseases or degenerative diseases including neurodegenerative diseases. Embodiments of the present disclosure provide compounds related to very long chain polyunsaturated fatty acids, pharmaceutical compositions containing the provided compounds, and methods of treating a subject with a condition or disease using provided compounds or pharmaceutical compositions.

The invention relates to the detection of fatty acids. In a particular aspect, the invention relates to methods for detecting very long chain fatty acids and branched chain fatty acids by mass spectrometry.

The invention relates to a reinforced and toughened anti-bacterial polypropylene composition and a preparation method thereof. The composition comprises the following components in weight percent: 54% to 87.7% of polypropylene, 0.5% to 5% of a coupling-modified inorganic antimicrobial agent, 10% to 30% of ultra-high molecular weight polyethylene master batch, 1% to 8% of graft polypropylene; 0.2% to 1% of a stabilizing agent, 0.2% to 1% of a dispersing agent and 0.4% to 1% of anti-oxidant. According to the invention, ultra-high molecular weight polyethylene is prepared into ultra-high molecular weight polyethylene master batch under certain conditions, and the ultra-high molecular weight polyethylene master batch, the surface-modified inorganic antimicrobial agent, the polypropylene, the graft polypropylene and other additives are quickly mixed and extruded to be manufactured into a polypropylene composite material with high strength, high ductility and high mold resistance. Under the condition that the inorganic antimicrobial agent is added to endow excellent antibacterial activity to the polypropylene, organic very long-chain high-polymer reinforced and toughened polypropylene is added, so that the prepared polypropylene composite material not only has excellent mold resistance but also has outstanding mechanical characteristics.

Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds of leaves.

The invention provides a method for transforming vegetable oil into very-long-chain highly unsaturated fatty acid enriched bio-oil. According to the method, vegetable oil or illegal cooking oil serves as a biological culture medium ingredient, organisms capable of transforming and accumulating very-long-chain highly unsaturated fatty acid, such as thraustochytrium roseum and the like, are adopted, a very-long-chain highly unsaturated fatty acid enriched organism is obtained by culturing a living organism, and the very-long-chain highly unsaturated fatty acid enriched bio-oil is obtained by means of extracting. The vegetable oil can be used for quickly promoting growth of thraustochytrium roseum and increasing the content of grease of thraustochytrium roseum. According to the bio-oil obtained by means of bio-concentration transformation, very-long-chain highly unsaturated fatty acid which is not contained in original vegetable oil can be increased, and the bio-oil obtained by the transforming method is safe and reliable in comparison with illegal cooking oil and the like. The very-long-chain highly unsaturated fatty acid enriched bio-oil can be widely applied to various animal feed additives and healthy foods.

A method of producing a composition comprising a high concentration of very long chain polyunsaturated fatty acids (VLCPUFAs) from natural oils such as fish oil, squid oil, algal oil and krill oil. In addition, a composition comprising a high concentration of very long chain polyunsaturated fatty acids isolated from such natural sources; as well as to a process for isolating separate fractions of very long chain polyunsaturated fatty acids having identical chain lengths but different degrees of unsaturation from such highly concentrated compositions is disclosed.

The invention provides a gas chromatography-mass spectrometry detection method of three very long chain fatty acids in a human serum. The method comprises the following steps (1) carrying out pretreatment on a human serum sample to obtain a derivative product; (2) detecting the derivative product by using a gas chromatography-mass spectrometer; (3) carrying out qualitative analysis on each very long chain fatty acid in the human serum sample according to a total ion chromatogram and a mass spectrogram of the derivative product, and calculating the content of each very long chain fatty acid in the human serum sample according to a selective ion chromatogram, wherein each very long chain fatty acid comprises docosanoic acid, tetracosanoic acid and hexacosanoic acid. Compared with the background art, the pretreatment process disclosed by the invention is fast, sensitive and accurate.

The invention discloses the application of 2-hydroxypropyl-β-cyclodextrin (HPCD) in the preparation of medicines for treating X-linked adrenoleukodystrophy (X-ALD), and belongs to the field of biomedicine. The present invention injects 2-hydroxypropyl-β-cyclodextrin into wild-type and X-ALD model mice (ABCD1 gene knockout mice), and performs cell, adrenal gland and cerebellum tissue sections on cells injected with HPCD mice Filipin staining was performed to measure plasma total cholesterol, very long-chain fatty acids, body weight, and behavioral tests. It was found that HPCD can reduce the cholesterol levels of plasma, cerebellum, and adrenal glands to within the normal range, and reduce the level of VLCFA. Alleviated behavioral abnormalities caused by neurodegeneration in X‑ALD mice. Based on this result, it is shown that HPCD can treat X-ALD, and it can be used to prepare a medicine for treating X-ALD.

A method of producing a composition comprising a high concentration of very long chain polyunsaturated fatty acids (VLCPUFAs) from natural oils such as fish oil, squid oil, algal oil and krill oil. In addition, a composition comprising a high concentration of very long chain polyunsaturated fatty acids isolated from such natural sources; as well as to a process for isolating separate fractions of very long chain polyunsaturated fatty acids having identical chain lengths but different degrees of unsaturation from such highly concentrated compositions is disclosed.