298 results about "Iodo fatty acid" patented technology

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

The present invention provides a method to prepare a fatty acid lower alkyl esters from a feedstock, such as a vegetable or an animal oil, comprising one or more fatty acid glycerol esters such as mono-, di- or tri-glycerides, and free fatty acids, comprising combining the feedstock, a lower alcohol and an acidic mesoporous silicate under conditions wherein the mesoporous silicate catalyzes the formation of the corresponding fatty acid lower alkyl ester of the free fatty acids an optionally glycerol.

The present invention relates to extracted plant lipid or microbial lipid comprising docosahexaenoic acid, and / or docosapentaenoic acid, and processes for producing the extracted lipid.

A partially hydrogenated fatty substance produced by partial hydrogenation of a substrate, such as vegetable, animal or marine oil, said partially hydrogenated substance having a low content of trans fatty acids. When the hydrogenation degree is below 30% the trans-fatty acid concentration can be expressed in the following way: trans<=0.3x(initial IV-IV) in %. of the total amount of fatty acids, wherein IV refers to iodine value. When the hydrogenation degree is between 30 and 70% the trans-fatty acid concentration can be expressed with: trans<=0.09xinitial IV in % of the total amount of fatty acids. The partial hydrogenation is performed by a process in which the substrate, hydrogen gas and a solvent are mixed, and the whole mixture is brought into a supercritical or near-critical state. This substantially homogeneous super-critical or near-critical solution is led over the catalyst, whereby the reaction products formed, i.e. the hydrogenated substrates, will also be a part of the substantially homogeneous super-critical or near-critical solution.

The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and / or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and / or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and / or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase.

The invention relates to a method for preparing glyceride rich in algal oil n-3 polyunsaturated fatty acid through the enzyme process. The method comprises the following steps: taking microbial algal oil as raw material, carrying out hydrolysis of part or all of algal oil respectively through controlling the hydrolysis rate under the catalysis of lipases to obtain glyceride partially hydrolyzed and free fatty acid; enabling the free fatty acid to enrich n-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) through the urea adduct method to obtain an n-3 polyunsaturated fatty acid condensate; and taking the n-3 polyunsaturated fatty acid condensate and glyceride partially hydrolyzed as substrates, taking immobilized lipases as catalysts, and efficiently synthetizing glyceride rich in algal oil n-3 polyunsaturated fatty acid in an organic phase. The invention adopts a complete biological enzymatic enrichment process, the raw material is clean and safe, the process route is scientific and reasonable, the catalytic activity of enzyme is high, the catalyst can be recycled, the cost is low, the reaction condition is mild, the energy consumption is low, the environment is friendly, and the market prospect is wide.

The invention discloses a Raman-spectrum-based method for detecting the content of oleic acid, linoleic acid and saturated fatty acid in edible vegetable oil. The method comprises the following steps of: detecting and pre-treating a Raman spectrum of edible vegetable oil with known content of oleic acid, linoleic acid and saturated fatty acid; presetting a characteristic peak set, and performing affinity modeling on the Raman spectrum strength corresponding to the edible vegetable oil to be detected and the known content of oleic acid, linoleic acid and saturated fatty acid, thus obtaining the Raman spectrum of the edible vegetable oil to be detected; and pre-treating the Raman spectrum, extracting the Raman spectrum strength corresponding to the characteristic peak set, and substituting the Raman spectrum strength in a prediction module to obtain the content of the oleic acid, linoleic acid or saturated fatty acid in the edible vegetable oil to be detected. The method disclosed by the invention is simple in operation, high in detecting speed and capable of simultaneously predicting the content of the three fatty acids according to the prediction module; and compared with a real result detected by a gas chromatographic method (GC), the prediction result of the method provided by the invention is higher in precision.

The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.

The present invention pertains to bio-based block polymers synthesized from functionalized lignin-based molecules (A-monomer) and functionalized fatty acids or fatty alcohols (B-monomer) derived from plant or animal oils, waxes or fats. The block polymers can be synthesized via numerous polymerization techniques, such as reversible addition-fragmentation chain transfer (RAFT). Most importantly, this class of bio-based block polymers shows promise as providing sustainable yet scalable and tunable thermoplastic elastomers and pressure-sensitive adhesives, among other applications.

The present invention relates to a concentration- and purification method for compositions containing phytanic acid and / or derivatives thereof besides other fatty acids and / or their derivatives, preferably besides polyunsaturated fatty acids and / or their derivatives, such as esters or salts. With the fractionation process the phytanic acid (derivative) can become concentrated and the fatty acid mixture, preferably PUFA-mixture, can be purified from this particular component.

The invention relates to a preparation method of conjugated linoleic acid, comprising the following steps: placing the vegetable oil containing linoleic acid glyceride or mixed fatty acid containing linoleic acid or extracted and separated linoleic acid product as raw material, low boiling point alcohols solvent and base catalyst into a reaction kettle and performing the isomerization reaction at a certain temperature. The reaction product is subjected to evaporating, acidifying, extracting and vacuum drying to obtain the conjugated linoleic acid. The conversion of the linoleic acid is above 97.5% and the total yield of the conjugated linoleic acid is above 85%, the proportions of the cis-9, trans-11 isomer and trans-10, cis-12 isomer in the total conjugated linoleic acid are above 96%. The preparation method has features of less reaction steps, easy recovery and repeated use of solvent, simple follow-up separation, low production cost, and easy industrialization.

The invention relates to a technological method for preparing an epoxy fatty acid methyl ester plasticizer with waste vegetable oil. The method comprises the steps of: subjecting impurity removed mixed swill-cooked dirty oil or catering hogwash oil and other waste vegetable oil to molecular distillation directly in a distillation column for removing fatty acid so as to convert the oil to neutral oil with an acid value of 0.8-1.2; then adding 1-3% sodium methoxide and 20-25% methanol, raising the temperature gradually to 66DEG C to 70DEG C under stirring for a reaction of 40-50min, leaving the oil to stand for 25-35min, and discharging crude glycerol, thus obtaining fatty acid methyl ester; then adding 2-4% formic acid, raising the temperature gradually to 45-55DEG C under stirring, starting to add 20-30% hydrogen peroxide dropwisely for a reaction of 6.5-7.5h, thus obtaining epoxy fatty acid methyl ester; then adding 15-25% sodium hydroxide liquid for neutralization so as to make the PH of the material ranging from 6.5 to 7.5, carrying out washing and distillation, thus obtaining the high-purity, non-toxic and colorless epoxy fatty acid methyl ester plasticizer. The preparation method of the invention has simple process and low cost, and can substitute o-benzene plasticizers to a greater degree.

The invention relates to a method for preparing fatty alcohol from fatty acid or fatty acid ester by selective hydrogenation. In a hydrogen atmosphere, fatty acid or fatty acid ester used as the raw material is subjected to a selective hydrogenation process in a solvent under the catalytic action of a supported catalyst to perform selective conversion, thereby obtaining the corresponding saturated fatty alcohol. In the method, the raw material conversion rate is up to higher than 90%, and the fatty alcohol selectivity is up to higher than 98%. The invention also relates to a supported catalyst for preparing fatty alcohol from fatty acid or fatty acid ester directly by selective hydrogenation. The method has the advantages of high conversion rate, high selectivity, favorable recovery performance of the catalytic system, simple and environment-friendly technique and the like, is suitable for industrial large-scale production, and has wide application prospects.

Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.

Method and apparatus for esterification of fatty acids or fatty acids contained in fats and oils with lower monovalent alcohol having 1 to 4 carbon atoms, comprising combining in a reaction section the fatty acids or fatty acids contained in fats and oils, and the alcohol to form a mixture, the combining forming enlarged interphases of the mixture by high or powerful dynamic shear forces and turbulence; at least one of strong mineral acid and acid ion exchange resin being present in the reaction section or a post-reaction section; starting esterification in the reaction section under pressure of 2 to 500 bar, and reducing pressure during the esterification so that a high interphase is maintained; and the esterification is carried out in the reaction section at a temperature of 50 to 300° C.

The invention relates to a fatty acid component microcapsule and a preparation method thereof, and belongs to the field of food and medicine. The microcapsule consists of crude oil containing fatty acids and auxiliary materials, wherein the auxiliary materials consist of wall materials, an antioxidant and an emulsifier. The microcapsule consists of the following ingredients by weight: 30-50% of the crude oil, 34-60% of the wall materials, 2-10% of the antioxidant and 6% of the emulsifier. According to the fatty acid component microcapsule and the preparation method thereof, the microcapsule with excellent water-solubility is prepared from palmitic acid (PA), oleic acid (OA), linoleic acid (LA), alpha-linolenic acid, eicosapentaenoic acid (EPA) and docosahexenoic acid (DHA). The microcapsule can be directly taken with water and can also be used for clinical patients in nasal feeding and tube feeding to improve biological digestibility, absorptivity and biopotency.

The invention provides a preparation method of a polyglycerol fatty acid ester. The preparation method comprises the steps of firstly obtaining mixed polyglycerol through polymerization reaction of glycerol under the catalysis of an KF-SnCl2 / Al2O3 supported solid base catalyst, conducting purifying treatment on the mixed polyglycerol by molecular distillation to obtain relatively pure polyglycerol, and then preparing the polyglycerol fatty acid ester through esterification reaction of the purified polyglycerol and liquid fatty acid. The polyglycerol obtained by the preparation method is concentrated in polymerization degree and is mainly of a linear chain structure, and the produced polyglycerol fatty acid ester not only has good taste, light smell and light color and luster, can reach thesame level of imported products, meets the national food additive safety standards, has strong emulsifying capability and meets the performance requirements and food safety requirements of various emulsifying systems. In addition, the polymerization reaction temperature of the preparation method is low, the production cost is reduced, and the preparation method is simple in operation and suitablefor large-scale industrial production.

The invention discloses a method for preparing mixed fatty acid by using waste cooking oil and the application method of the mixed fatty acid. The preparation method comprises: 1) the waste cooking oil is pretreated; 2) concentrated sulfuric acid is added into an oil layer which is then turned over and cooked and stands; the mixture is washed by hot water and stands, and the waste water at the lower layer is discharged; the obtained product is washed by water repeatedly until acidated oil with the pH value of 6-8 is obtained; 3) the acidated oil, distilled water, sulphuric acid and a catalyst are added in a reaction vessel, heated, stirred, and hydrolyzed and then stand; the waste water at the lower layer is discharged; the mixture is washed by hot water and stands, and the waste water at the lower layer is discharged; the product is washed by water repeatedly until the discharged water is neutral; 4) the step 3 is carried out again, and crude fatty acid can be obtained by secondary hydrolysis; 5) the mixed fatty acid product is obtained by thermal dehydration and negative pressure distillation. The method has high synthetic efficiency, low requirements for equipment as well as low investment and preparation cost; the prepared mixed fatty acid has high activity, strong fluidity and remarkable flotation performance when being applied to the aspect of ore dressing flotation, thereby having stronger competition strength and good application prospect.

The invention discloses a method for determining compositions of fatty acids and acylglycerols at alpha and beta positions of structured lipid by combining solid-phase extraction and gas chromatography. The method comprises utilizing pure arachidonic acid-triacylglycerol (ARA-TAG), docosahexaenoic acid-diacylglycerol (DAH-DAG), linolenic acid- monoacylglycerol (Ln-MAG) and an oleic acid-free fatty acid (O-FFA) standard sample, and optimizing eluent elution conditions with different polarity ratios, so as to effectively separate TAG, DAG, MAG and FFA in mixtures in a same SPE (Solid Phase Extraction) microcolumn; and the method is characterized by also comprising the following steps: 1) separation of a mixed standard sample and analysis on compositions of fatty acids; and 2) analysis on compositions of fatty acids and acylglycerols at alpha and beta positions of the structured lipid. The method has the advantages of simple operation, low sample demanded amount, less consumption amount of a needed solvent, accurate qualitative quantitative analysis results, low detection limit and short analysis time, and is capable of realizing high-flux detection on TAG, DAG, MAG and FFA compositions and content in structured lipid and compositions of fatty acids at alpha and beta positions of structured lipid.

The invention relates to an extraction and purification method of the pupa alpha-linolenic acid with the supercritical CO2 technology. The natural biological pupa powder is used as the raw material; the supercritical CO2 is used as the extraction agent to get the pupa oil; the pupa oil is added into the sodium hydroxide alcohol solution, and then to be stired, water-added and clarified; besided, the pupa oil is extracted by the organic solvent; the mixed fatty acid and urea are put into the urea methanol solution; at minus 15 to minus 20 Celsius system, and then to be stired 12 to 36 hours, next to be filtered and washed with the methanol;finally, the product is dried and the product can be got. The invention can be operated at a low temperature, avoiding the damage to the polyunsaturated fatty acid; the purity of the product quality is high and the extraction time is short; the operation is simple and convenient; compared with the traditional solvent method and squeezing method, the collection rate is high and the efficiency is high; the CO2 can be reused; no solvent is left; the product quality is high; the invention can be used in the mass production of the industrial mechanization.

The invention relates to a fatty acid composition, which is diluted at normal temperature and in cold water to form solution used for cleaning of sterilization, fungicide and antivirus. The fatty acid composition comprises at least the following components: component A: food fatty acid comprising at least octyl decanoic acid and nonanoic acid; component B: surfactant comprising alkane sulfonate; component C: acid. The fatty acid composition has the advantages that the fatty acid composition or the diluted solution thereof has good stability, bactericidal and antiviral activity under the water temperature of 4-20 DEG C, no phase separation or crystallization, thus having safer use performance, and active components are low in cost, having more broad-spectrum bactericidal activity which can kill various bacteria, epiphytes and viruses. The fatty acid composition or the diluted solution thereof can be used for sterilization, fungicide and antivirus, which has high-efficient and quick killing effect; the sterilizing rate can reach more than 99.999 percent with contact time less than 1 minute; The fatty acid composition or the diluted solution thereof also has strong cleaning ability, and can keep effective even in hard water; the killing ratio for poliovirus reaches 99.9999 percent and the poliovirus can be killed immediately.

The invention concerns compositions containing polyunsaturated fatty acids (PUFA's). More particularly it concerns a composition containing PUFA's, (particularly long chain PUFAs derived from marine sources, particularly fish, and its use in fortifying consumables, such as food, drink, supplements and nutraceutical or pharmaceutical products. More particularly it is a substantially non aqueous additive mix, for introducing poly unsaturated fatty acids into ingestible products, which when hydrated forms an oil in water emulsion, comprising one or more poly unsaturated fatty acids or a source thereof and one or more emulsifying agents. The mix can be added to, particularly farinaceous products, to provide PUFA supplementation.

The invention relates to a method of extracting unsaturated fatty acid from abdominal fat of geese with fatty liver. The method comprises the steps of preparation of crude goose oil of abdominal fat of geese with fatty liver, refining of the crude goose oil and extraction of unsaturated fatty acid, and is characterized in that: there is a step of extraction of goose oil mixed fatty acid between the step of refining of the crude goose oil and the step of extraction of unsaturated fatty acid. According to the invention, refining technological parameters of degumming, decolouring and deodorization for the goose oil are improved in the method, thereby removing non-glyceride components in the unsaturated fatty acid, substantially abating influence of impurities on subsequent processes and improving stability of the unsaturated fatty acid; the step of extraction of goose oil mixed fatty acid is added before the step of extraction of unsaturated fatty acid, thereby substantially improving efficiency of subsequent extraction of the unsaturated fatty acid and purity of the unsaturated fatty acid and reducing cost. The method enables waste abdominal fat of geese with fatty liver to be fullyutilized, having an important social benefit and economic value. The extracted unsaturated fatty acid can be used in a wide variety of fields like foodstuffs, medicines and cosmetics.

The invention provides transgenic photosynthetic microorganisms that include non-native genes encoding polypeptides having lipolytic activity for production of free fatty acids and fatty acid derivatives, and methods of producing free fatty acids and fatty acid derivatives using the transgenic microorganisms disclosed herein. The invention also provides transgenic microorganisms that include non-native genes encoding polypeptides having lipolytic activity, and novel genes encoding polypeptides demonstrating lipolytic activity.