Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for determining very long chain fatty acids in sample by liquid chromatography tandem mass spectrometry

A very long chain fatty acid and liquid chromatography technology, which is applied in the field of liquid chromatography tandem mass spectrometry for the determination of very long chain fatty acids in samples, can solve the problem of not finding VLCFA by liquid chromatography tandem mass spectrometry, and achieves increased detection specificity and avoidance of The effect of interference, reducing sample size

Inactive Publication Date: 2019-05-10
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
View PDF9 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method for detecting VLCFA by liquid chromatography tandem mass spectrometry, and there is no matching kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for determining very long chain fatty acids in sample by liquid chromatography tandem mass spectrometry
  • Method and kit for determining very long chain fatty acids in sample by liquid chromatography tandem mass spectrometry
  • Method and kit for determining very long chain fatty acids in sample by liquid chromatography tandem mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Method for Determination of Serum Very Long Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry

[0042] 1. Experimental reagents

[0043] The sample preparation solution includes: isotope internal standard solution, acetonitrile, concentrated hydrochloric acid (12mM), n-hexane, methanol (reconstituted solution).

[0044] Isotope internal standard solution: C22:0-d4 solution: concentration is 10.2ng / ml; C24:0-d4 solution: concentration is 23.2ng / ml; C26:0-d4 solution: concentration is 28.5ng / ml.

[0045] Instrument analysis fluids include:

[0046] Mobile phase A: Acetonitrile in water (v / v, 6:4) solution containing 5 mM ammonium formate.

[0047] Mobile phase B: acetonitrile isopropanol (v / v, 8:2) solution.

[0048] 2. Sample preparation

[0049] Take 50μl serum sample or very long chain fatty acid (C22:0, C24:0 and C26:0) standard solution to 10ml urinary tube, add 10μl isotope internal standard, mix and shake, add 200μl acetonitrile, add 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Linearaaaaaaaaaa
Login to View More

Abstract

The invention provides a method and kit for determining very long chain fatty acids in a sample by liquid chromatography tandem mass spectrometry. The method comprises the steps that very long chain fatty acid standardized product solutions with different concentration are prepared; stable isotope internal standards are added to the standardized product solutions to be mixed uniformly, and acetonitrile and concentrated hydrochloric acid are added for sample pretreatment; then n-hexane is used for extraction, nitrogen is used for blowing dry supernatant, and liquid chromatography tandem mass spectrometry detection is performed after redissolution; a standard curve is drawn according to the concentration of the standardized product solutions and the detected signal intensity; the standardized product solutions in the step B are replaced with the sample to be detected for detection; according to a detection result of the sample to be tested and compared with the standard curve, the concentration of the very long chain fatty acids in the sample to be detected is obtained, and thus, the quantitative detection of the very long chain fatty acids in the sample is realized. The method for determining the very long chain fatty acids in the sample by liquid chromatography tandem mass spectrometry is successfully established, and the method can quickly and specifically detect the content of the very long chain fatty acids in samples (especially serum samples) with a small use amount of samples.

Description

technical field [0001] The invention relates to liquid chromatography tandem mass spectrometry detection technology, in particular to a method and a kit for liquid chromatography tandem mass spectrometry determination of very long-chain fatty acids in samples. Background technique [0002] Peroxisome diseases are inherited metabolic disorders caused by defective peroxisome biogenesis or deficiency of a single peroxisome. Peroxisome diseases are composed of many diseases, the most common of which is adrenoleukodystrophy (X-linked adrenoleukodystrophy, X-ALD). The disease shows that the oxidation of VLCFA by peroxisomes in the cells is impaired, so that VLCFA accumulates in a large amount in blood, white matter, adrenal cortex and other organs and tissues, causing demyelination of the central nervous system and adrenal cortex shrinkage or dysplasia. , mainly involving the adrenal glands and white matter. Serum very long chain fatty acid (VLCFA) is mainly composed of 22 and m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/72
Inventor 王丹晨禹松林邱玲
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com