31results about How to "Less prone to pollution" patented technology

The invention discloses a method for culturing Pleurotus ferulae through corncob, which includes the following process: preparing materials, bagging, sterilizing, culturing, building wall and earthing, and then outputting; the method for culturing Pleurotus ferulae through corncob has the advantages that the biology transformation ratio of the Pleurotus ferulae is improved from 40-50 percent to 80-85 percent and the production cost is reduced from the original cost about 1.32 Yuan / bag to the cost about 1.08 Yuan / bag; the costing product quality of the Pleurotus ferulae is obviously improved and the taste is more excellent; the method for culturing Pleurotus ferulae through corncob after improvement is advanced in technology; the process is simple and rational; Pleurotus ferulae bags are not easy to be polluted; the finished product rate is high; the positivity of farmers to culture the Pleurotus ferulae is higher and the profit is more obvious.

The invention belongs to the field of microbial fermentation engineering, and specifically relates to a high-density culture method of clostridium thermocellum. The method is characterized in that a GS-2 culture medium is used as an initial culture medium; the fermentation medium is optimized through a multivariate statistical method; and cell concentration of clostridium thermocellum is rapidly and efficiently raised. The invention has the following advantage: the invention provides a fermentation medium for rapid acquisition of large cell concentration of clostridium thermocellum, thus laying the groundwork for subsequent production of cellulosome which has high conversion efficiency of cellulose to bioethanol. Bacterial density of clostridium thermocellum is raised by 6-9 times in comparison with that of the initial culture medium. OD600 under the bottling condition reaches 8.85, and OD600 in a fermentation cylinder reaches 12.7.

The invention relates to an open type simplified culture medium for taxus cuspidate. The culture medium comprises a primary culture medium and a subculture medium. The primary culture medium is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, active carbon AC and agricultural streptomycin into optimized B5 basic mother liquor. The subculture is prepared by adding and mixing carrageenan, white granulated sugar, indolebutyric acid IBA, 6-benzyl amino adenine BA, active carbon AC and agricultural streptomycin into optimized MS basic mother liquor. According to the culture medium provided by the invention, with the adoption of open type simplified tissue culture, the cost and the inoculating time of the culture medium are remarkably saved, the autoclaved sterilization program is cancelled, and the production flow is simplified by replacing autoclaved sterilization and strict enclosed environment operation by a bacteriostatic agent, so that the labor and equipment investment in industrialized seedling production can be reduced, and the fund is saved by 30-50%.

The invention relates to the field of nutrient solution bottle cleaning equipment, and discloses a cup and bottle cleaning machine which comprises a supporting rack, a conveying device, a driving plate device, a bottle opening cleaning device and a bottle body cleaning device. The supporting rack comprises a supporting frame and a workbench, the workbench is fixed on the supporting frame, the workbench is provided with a conveying channel, the conveying device is connected with one end of the conveying channel, the driving plate device, the bottle opening cleaning device and the bottle body cleaning device are fixed on the workbench, the conveying channel penetrates through the driving plate device, the bottle opening cleaning device and the bottle body cleaning device in sequence, the bottle opening cleaning device is used for cleaning bottle openings of nutrient solution bottles, and the bottle body cleaning device is used for cleaning bottle bodies of the nutrient solution bottles.When the nutrient solution bottle is cleaned, the bottle opening and the bottle body of the nutrient solution bottle are separately cleaned so that the cleaned nutrient solution bottle is cleaner.

The invention discloses a preparation method of skin fibroblasts, and belongs to the technical field of biological cells. The preparation method of the skin fibroblasts comprises the following steps of: S01, specimen treatment: treating a skin specimen, and removing adipose tissues and connective tissues; S02, separation: treating the treated specimen with neutral protease, and removing epidermis to obtain dermis tissues; S03, digestion: digesting the dermis tissues by using pancreatin to obtain tissue fluid; S04, centrifugation: centrifuging the tissue fluid, and removing supernatant to obtain a cell suspension; S05, culture: transferring the cell suspension into a culture dish, adding a culture solution, and culturing; and S06, passage: culturing until the fusion degree is 75-85%, and performing passage culture. The preparation method disclosed by the invention has the advantages of simple steps, low possibility of pollution, a high cell survival rate, a good purification effect and small cell loss.

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

The invention provides a method for treating heavy metal wastewater by virtue of a conducting film. The method comprises the following specific steps: 1, pumping heavy metal wastewater entering from a wastewater inlet (1) to a pre-treatment device (3) through a pump (2) to remove particles in wastewater; 2, introducing the wastewater without particles to a pH adjusting pool (4) to adjust the pH value of the wastewater to 4-6.5; and 3, pumping the wastewater with adjusted pH value to a film pond (9) through a valve (5), a flowmeter (6) and a pressure gauge (7) by means of a pump (2'), wherein heavy metal ions can not penetrate through a conducting film to form heavy metal concentrated liquor due to the positive electricity rejection effect between the conducting film as an anode in the film pool (9) and the heavy metal ions in the wastewater, while water in the wastewater penetrates through the conducting film to form a penetrating fluid. The heavy metal concentrated liquor flows back to the adjusting pool (4) through a pressure gauge (7') and a valve (5') while the penetrating fluid is discharged through a penetrating fluid outlet (8).

The invention belongs to the field of microbes, and particularly relates to a method for continuously culturing bacillus subtilis and a special fermenting system. The method provided by the invention comprises the following steps: (1) transferring stored bacillus subtilis strain to a test tube agarslantculture-medium, performing strain activation, and then, transferring the strain into a triangular flask and a seeding tank for culturing in sequence to prepare a bacillus subtilis seed; (2) taking culture materials including starch, corn powder, soya bean meal, peptone, NaCl, CaCl2, MgSO4.7H2O, KH2PO4, K2HPO4, MnSO4, a defoaming agent and water; (3) after the culture materials in the step (2) are subjected to sterilizing treatment, inoculating the activated and cultured bacillus subtilis seed, and performing three-stage continuous culturing to obtain a fermenting liquid. The bacillus subtilis cultured with the method provided by the invention is stable and uniform in quality; neither mixed fungi pollution nor strain mutation is easily caused; the service life of various detectors is prolonged; the time is reduced; the yield and the production efficiency are improved; the concentration and the spore rate of thalli of the bacillus subtilis are improved.

The invention relates to a biochemical treatment method and device for landfill leachate. The method specifically includes: firstly, the pretreated landfill leachate is put into an anaerobic biological filter for treatment, and then undergoes first-level facultative biochemical treatment, anoxic reaction After nitrification treatment and secondary aerobic biochemical treatment, the water is finally discharged through the ceramic membrane module for the subsequent advanced treatment process. The present invention has a good treatment effect, adopts anaerobic-facultative oxygen-anoxic-aerobic microbial environment to form different microbial flora, effectively improves the effect of biochemical treatment of landfill leachate, and reduces the pressure of subsequent advanced treatment. It reduces the investment and operating costs of the overall process; at the same time, it can be combined with adsorption process or reverse osmosis technology to ensure the discharge of landfill leachate up to the standard.

The invention relates to the technical field of nuclear fuel aftertreatment and particularly discloses a device for measuring americium and cesium in aftertreatment feed liquid. A NaI detector is arranged in an area defined by central holes of a lead sheath A, a lead sheath B and a lead sheath D, and a connecting line of the NaI detector penetrates out of a slot in the lead sheath B to be connected with a multi-channel spectrometer; the multi-channel spectrometer is connected with a computer through a data line; and gamma ray signals are converted into electric signals by the NaI detector, theelectric signals are screened by the multi-channel spectrometer and then transmitted into the computer, and automatic sample measuring is realized. The device can effectively shield interference of environmental gamma rays in a measuring system, the multi-channel spectrometer is utilized, thus the gamma radioactivity concentrations of cesium-137 and americium-241 can be measured simultaneously, and the measuring result is directly read by software.

The invention discloses an apparatus for preparing an ion probe indium target and a preparation method of the ion probe indium target. The apparatus includes a target support, a heating mechanism for heating the target support, a pressure rod, a sliding rod, an air cylinder, a first support frame for supporting the air cylinder, a second support frame for supporting the pressure rod and a third support frame for supporting the sliding rod. One end of the pressure rod is movably connected to the top of the second support frame while the other end is connected to the air cylinder. The sliding rod and the heating mechanism are both arranged below the pressure rod. One end of the sliding rod faces to the pressure rod while the other end faces to the heating mechanism. The air cylinder applies pressure onto the pressure rod longitudinally, thereby driving the sliding rod to apply a pressure onto the heating mechanism longitudinally. The apparatus can increase the micron resolution ratio from more than 5 [mu]m to less than 1 [mu]m, thereby achieving measurement on age and microelements of a narrow metamorphic edge (less than 5 [mu]m) of low-grade metamorphic zircon. The surface flatness of prepared indium target is less than 10 [mu]m and the indium target can satisfy a requirement on flatness during ion probe detection.

The invention discloses a special thermophilic bacterium for catalytic production of alpha-ketoglutaric acid and a catalytic production method. The strain is Streptomyces sp.TC2303 and is preserved in the China Center for Type Culture Collection on December 27, 2021, the preservation date is December 27, 2021, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20211682. The culture and fermentation conditions of the strain Streptomyces sp.TC2303 provided by the invention are both about 45 DEG C, the fermentation time is only 24 hours, and the fermentation time is short; according to the present invention, the fermentation broth can be used to catalyze L-glutamic acid at the temperature of 45-56 DEG C to produce alpha-ketoglutaric acid, the conversion process temperature is more than 45 DEG C, the catalysis temperature is far higher than the prior art, the total reaction time is only 18 h, the catalysis process is not easily subjected to bacterial contamination, and the method has special advantages for enterprises with simple and crude equipment and easy bacterial contamination.

The invention relates to the field of application of medical instruments and discloses a tissue filtering method for extracting cartilage cells, the method is carried out in a tissue filtering device, the tissue filtering device comprises a tubular part (1), a filter screen (2) and a clamping part (3); the filter screen (2) is arranged at one end of the tubular part (1) and covers an opening located at one end of the tubular part (1); the clamping part (3) is arranged on the inner wall of the other end of the tubular part (1), and the clamping part (3) is arranged to be convenient for a user to clamp and fix by using a corresponding clamp. The tissue filtering device applied in the method has the advantages of being low in cost, convenient to operate and not prone to pollution.

The invention discloses a disposable laparoscope flushing and sucking tube device. The device comprises a main body structure, wherein the main body structure comprises a first hose, a second hose and a branch tube, the second hose and the first hose are arranged side by side, a side portion of the second hose is fixedly connected with a side portion of the first hose, the branch tube is fixedly installed on the side portion of a side, away from the first hose, of the second hose, and the branch tube communicates with interior of the second hose. According to the disposable laparoscope flushing and sucking tube device, the first hose, the second hose and the branch tube are integrated, the device can be used as a flushing, sucking and smoke discharging pipeline special for a laparoscopic surgery in clinic, is convenient to use, clean and orderly, is not easy to be polluted during use, and effectively avoids risk of postoperative infection; and the branch tube can timely discharge smoke generated when electrosurgical equipment is used, so that a clear surgical field can be ensured, and harm of the smoke to medical personnel can be effectively reduced.

Disclosed in the invention is an electrode lead protection device comprising a clamping part and an encircling part. The clamping part being solid is provided with a first limiting structure. The encircling part is formed by extending of the clamping part and contains an accommodation cavity having an opening; and the encircling part is provided with a second limiting structure. According to the invention, the solid clamping part and a limiting element arranged on the clamping part provide clamping points for an auxiliary operation tool and the hollow encircling part is designed to accommodate an electrode lead, and the limiting structure is designed for the encircling part to realize operation bundling, so that a phenomenon that the electrode lead is damaged due to direct contact with the auxiliary tool or an operation thread can be avoided. Besides, the electrode lead connecting end can be protected from being polluted when passing through various subcutaneous tunnels or in a subcutaneous implanting mode during the implanting process.

The invention provides a large-size sapphire boat transfer device, which comprises an assembly platform, a piece push mechanism and a boat loading protection mechanism, wherein the assembly platform is provided with a boat positioning mechanism and a wafer support mechanism; the piece push mechanism comprises a slide bar, a slide block, a handle, a push rod and a piece push bracket; the boat loading protection device comprises a positioning lever, a lever groove, a connecting rod, a connecting rod through hole, a movable button, a movable button spring and a movable button groove. By the wafer transfer device provided by the invention, the risks of human contact, man-made pollution, drop breakage and the like caused in the wafer transfer process can be effectively reduced. A wafer is transferred in a manner of point contact of an insulating material and the wafer all the way when transferred, and the contact of a human body and the wafer is completely avoided, so that the man-made pollution is avoided from the source.

The invention relates to the field of production technology of a vegetable protein beverage, and aims to provide a production technology of red date and rose soymilk for women. The production technology mainly comprises the following steps of: (1) soybean screening; (2) soaking; (3) washing; (4) soymilk grinding; (5) soymilk boiling; (6) filtering; (7) syrup preparation; (8) mixing; (9) homogenizing; (10) sterilizing; (11) sterile filling and the like. Through the production technology provided by the invention, the obtained soymilk has high content of nutritional ingredients, full and smoothmouthfeel and low energy consumption; and moreover, the product quality is easy to control, and the production process is safe and reliable.

The invention belongs to the field of microbes, and particularly relates to a Bacillus licheniformis continuous culture method and a special fermentation system. The method comprises the following steps: (1) inoculating a Bacillus licheniformis strain into a test tube slant culture medium, activating the strain, sequentially inoculating into a triangular flask a seeding tank, and culturing, thereby preparing the Bacillus licheniformis seeds; (2) taking a culture medium composed of starch / glucose, corn flour, bean pulp powder, (NH4)2HPO4, NaCl, CaCl2, MgSO4.7H2O, KH2PO4, K2HPO4, MnSO4, a defoaming agent and water; and (3) sterilizing the culture medium in the step (2), inoculating and activating the cultured Bacillus licheniformis seeds, and carrying out three-stage continuous culture, thereby obtaining the final fermentation liquid. The method implements high mechanization and automation, and the Bacillus licheniformis has stable and uniform quality and can not have the phenomena of infectious microbe pollution and strain variation. The method prolongs the service lives of detecting instruments, saves the time, enhances the yield and production efficiency, and increases the Bacillus licheniformis strain concentration and spore rate.

The invention relates to a luminous warning road reflective paint and a construction method thereof. The coating is composed of a primer and a topcoat; by weight percentage, the A component of the primer includes: two-component room temperature curing acrylic resin, luminous powder, leveling agent, wetting and dispersing agent, the first defoamer, catalyst Drying agent, toner, reactive diluent, primer B component includes: initiator, reactive diluent; topcoat A component includes: two-component room temperature curing fluorocarbon resin, glass beads, anti-settling agent, second 2. Defoamer, drier, solvent; topcoat component B includes: curing agent, thinner. The coating formed by the coating of the present invention has a good surface state, is not easily polluted, has good reflection effect, strong adhesion, and the marking line is not easily deformed, and has good stability at the same time, which is beneficial to reducing the later maintenance cost.

The invention discloses an electrode for preparing a piece of cover glass. The electrode comprises an electrode containing an additive, the electrode is installed at a pool wall of a melting pool, andthe other side of the electrode is provided with a water cooling plate; the electrode employs stannic oxide as a base-material, the additive comprises antimony oxide, zirconium oxide and copper oxide, and the mass of the zirconium oxide is 1.8-2.2% of the total mass of the electrode. The electrode for preparing the cover glass comprises the electrode containing the additive, the electrode is installed at a pool wall of a melting pool, the other side of the electrode is provided with a water cooling plate; the electrode employs stannic oxide as a base-material, the additive comprises antimonyoxide, zirconium oxide and copper oxide, ZrO2 is added into the stannic oxide electrode material, so that the electrode has higher usage temperature, high resistance to oxidation, and better erosion resistance; the product can be operated at 1600 DEG C for a long time, service lives of the electrode and a tank furnace are prolonged, compactness of the electrode material is increased, and falling of blocks and pollution on glass liquid are not easy to happen in production.

The invention provides a method for treating heavy metal wastewater by virtue of a conducting film. The method comprises the following specific steps: 1, pumping heavy metal wastewater entering from a wastewater inlet (1) to a pre-treatment device (3) through a pump (2) to remove particles in wastewater; 2, introducing the wastewater without particles to a pH adjusting pool (4) to adjust the pH value of the wastewater to 4-6.5; and 3, pumping the wastewater with adjusted pH value to a film pond (9) through a valve (5), a flowmeter (6) and a pressure gauge (7) by means of a pump (2'), wherein heavy metal ions can not penetrate through a conducting film to form heavy metal concentrated liquor due to the positive electricity rejection effect between the conducting film as an anode in the film pool (9) and the heavy metal ions in the wastewater, while water in the wastewater penetrates through the conducting film to form a penetrating fluid. The heavy metal concentrated liquor flows back to the adjusting pool (4) through a pressure gauge (7') and a valve (5') while the penetrating fluid is discharged through a penetrating fluid outlet (8).

The invention relates to the technical field of fluorescence polarization, in particular to a method suitable to detect mononucleotide polymorphism in a fluorescence polarization technology. In order to overcome the problems of high cost, complex operation step, easy pollution and detection result influence in the prior art, the technical scheme is achieved as follows: reagents adopted by the method comprise MgCl2, 10* reaction buffer, TapDNA polymerase, dNTPs, target gene DNA primer, and a DNA probe with fluorescence labeling at the 3 end of a target zone; and the reagents are performed with amplified reaction. The invention has the advantages that: firstly, the steps and the operation are simple, the amplified reaction is performed in a hybrid stopped tube by one step without easy pollution and accurate result; secondly, the result analysis is simple and the result is judged by only number comparison so as to be easy to standardize and automate, and the application range is wide; thirdly, the cost is low without any special reagent and fluorescent quenching or microgroove wedding agent.

The invention belongs to a construction technology welding method for building engineering. In particular, it refers to the welding installation of bimetal composite steel pipes (SFG) in stations or long-distance pipeline projects, which includes the following steps: pipe groove processing, base pipe and liner gap girth welding, stainless steel liner butt weld welding, Dissimilar steel butt joint welding, intermediate layer weld seam filling welding, surface layer weld seam cover welding, bimetallic composite steel pipe solves the problem of oil field sewage transportation well, has strong applicability and high technological advancement, While fully meeting the requirements of oilfield sewage transportation, it can effectively reduce the user's operating costs, minimize safety hazards, and has a very broad application prospect.

The invention discloses an electrode lead protection device, which includes a clamping part and a surrounding part, the clamping part is solid, the clamping part is provided with a first limiting structure, and the surrounding part is extended from the clamping part Formed, the enclosing portion includes an accommodating cavity with an opening, and the enclosing portion is provided with a second limiting structure. The present invention designs a solid clamping part and the stopper on it to provide a clamping site for surgical auxiliary tools, and designs a hollow surrounding part to accommodate electrode wires, and at the same time designs a limiting structure in the surrounding part for surgical binding, thus protecting The electrode lead will not be damaged due to direct contact with auxiliary tools or surgical wires, and at the same time, the electrode lead connection end is protected from contamination when it passes through various subcutaneous channels or is implanted under the skin.

The invention provides a hydrophilia polypropylene hollow fiber membrane and a preparation method thereof, and relates to the technical field of membrane materials. The preparation method comprises the steps of casting membrane liquid preparation, thermally induced phase separation spinning and post treatment, a hydrophilic polymer, an initiator, a polymer, a diluents and a non-diluents are addedinto the casting membrane liquid, the hydrophilic polymer is grafted onto a polypropylene molecular chain in the preparation process of the casting membrane liquid by a method of in-situ polymerization grafting, uniform casting membrane liquid is formed, and then the hydrophilia polypropylene hollow fiber membrane is prepared by the methods of an extruder and a spinning machine, hot drawing, coagulation bath phase separation, heat treatment, solvent extraction and the like. According to the preparation method, the hydrophilic polymer is grafted onto polypropylene very conveniently and fast through a method of in-situ reaction, the hydrophilicityof the prepared hydrophilia polypropylene hollow fiber membrane can be significantly improved, excellent stability is achieved, water flux is large, and pollution is not prone being produced.

The invention belongs to the field of microbes, and particularly relates to a method for continuously culturing bacillus subtilis and a special fermenting system. The method provided by the invention comprises the following steps: (1) transferring stored bacillus subtilis strain to a test tube agarslantculture-medium, performing strain activation, and then, transferring the strain into a triangular flask and a seeding tank for culturing in sequence to prepare a bacillus subtilis seed; (2) taking culture materials including starch, corn powder, soya bean meal, peptone, NaCl, CaCl2, MgSO4.7H2O, KH2PO4, K2HPO4, MnSO4, a defoaming agent and water; (3) after the culture materials in the step (2) are subjected to sterilizing treatment, inoculating the activated and cultured bacillus subtilis seed, and performing three-stage continuous culturing to obtain a fermenting liquid. The bacillus subtilis cultured with the method provided by the invention is stable and uniform in quality; neither mixed fungi pollution nor strain mutation is easily caused; the service life of various detectors is prolonged; the time is reduced; the yield and the production efficiency are improved; the concentration and the spore rate of thalli of the bacillus subtilis are improved.

The invention relates to a multi-functional dual-layer fruit bagging body. The multi-functional dual-layer fruit bagging body comprises an outer-layer bagging body and an inner-layer bagging body andis characterized in that the inner-layer bagging body is placed in the outer-layer bagging body, and adopts an independent structure being in mutual separation from the outer-layer bagging body; the inner-layer bagging body is closed in circumferential direction, and is provided with an opened end in the upper side and a closed edge with a water draining hole in the lower side; vent holes are formed in the front and the rear surfaces of the inner-layer bagging body; a semicircle bag opening hole is formed in the front surface of the inner-layer bagging body; the outer-layer bagging body is closed in the circumferential direction, and is provided with an opened end in the upper side, a sealing binding wire in one side, and a closed edge with the water draining hole in the lower side, and vent holes are formed in the front and the rear surfaces of the outer-layer bagging body; and a semicircle bagging opening hole is formed in the front of the outer-layer bagging body. The inner-layer bagging body and the outer-layer bagging body adopt independent separation structures, bagging is needed once, after the outer-layer bagging body is taken out, the inner-layer bagging body is package, and secondary bagging is not needed, labor and time cost for secondary packing are saved, and the production efficiency is greatly improved.

The invention provides an SMT material suction device. The SMT material suction device comprises a first support and at least one material suction assembly arranged on the first support side by side, wherein each material suction assembly comprises a first mounting block, a first suction nozzle arranged on the first mounting block and a first driving mechanism pushing the first mounting block to move up and down; the first support is provided with a first guide mechanism matched with the first mounting block for up-down movement; and the first driving mechanism is in floating connection with the first mounting block through a first connecting block. Products can be automatically placed on the corresponding carrier tape or the packaging tray to be packaged, so that operation is convenient, the speed is high, and manpower and material resources are saved; and the first driving mechanism is in floating connection with the first mounting block through the first connecting block, and the connecting position of the first connecting block and the first mounting block can be properly and automatically adjusted, so that the safety and the reliability are high, the production efficiency and the yield of products are improved, the production control and the automatic online production of the products are facilitated, and the production cost is reduced.