A kind of continuous culture method of Bacillus licheniformis and special fermentation system
A technology of Bacillus licheniformis and culture methods, applied in the direction of microorganism-based methods, specific-purpose bioreactors/fermenters, biochemical equipment and methods, etc., capable of solving problems such as death and poor stress resistance of vegetative bodies
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Embodiment 1
[0064] A special fermentation system for the continuous culture method of Bacillus licheniformis, including a batching tank 1, a continuous elimination device 2, a maintenance tank 3, a cooling device 11, a feed liquid storage tank 5, a primary culture tank 6, and a secondary culture tank connected in series in sequence 8. Spore culture tank 9;
[0065] A seed tank 7 is connected in parallel between the cooling device 11 and the primary culture tank 6;
[0066] The spore culture tank 9 is connected with an auxiliary material tank 10;
[0067] The delivery pump delivery pump I4 is connected between the batching tank 1 and the continuous elimination device 2; the delivery pump delivery pump II12 is connected between the maintenance tank 3 and the cooling device 11;
[0068] There is a constant flow pump between the material storage tank, the primary cultivation tank 6, the secondary cultivation tank 8 and the spore cultivation tank 9.
[0069] The specific steps are to inocula...
Embodiment 2
[0071] The continuous culture method of Bacillus licheniformis comprises the following steps:
[0072] (1) Seed cultivation
[0073] a. Activation of strains on test tube slant
[0074] Transfer the preserved Bacillus licheniformis strains to the test tube slant medium, keep at 33°C, and cultivate for 30 hours to activate the strains;
[0075] The formula of test tube slant medium is: glucose 4g / L, peptone 3g / L, NaCl 1g / L, beef extract 0.5g / L, agar 20g / L, pH7.1, sterilized at 121°C for 20 minutes;
[0076] b. Erlenmeyer flask seed culture
[0077] Pick 1-2 rings of Bacillus licheniformis strains from the activated test tube and inoculate them into the Erlenmeyer flask, keep at 33°C, 120rpm after inoculation, and cultivate for 19 hours;
[0078] The medium formula of the triangular flask is: glucose 15g / L, soybean meal powder 16g / L, peptone 1g / L, NaCl 1g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 0.3g / L, pH7.1, sterilized at 121°C for 20 minutes;
[0079] c. Cultivation of 7 strai...
Embodiment 3
[0099] The continuous culture method of Bacillus licheniformis comprises the following steps:
[0100] (1) Seed cultivation
[0101] a. Activation of strains on test tube slant
[0102] Transfer the preserved Bacillus licheniformis strains to the test tube slant medium, keep at 32°C, and cultivate for 24 hours to activate the strains;
[0103] The formula of test tube slant medium is: glucose 4g / L, peptone 3g / L, NaCl 1g / L, beef extract 0.5g / L, agar 20g / L, pH7.0, sterilized at 121°C for 20 minutes;
[0104] b. Erlenmeyer flask seed culture
[0105] Pick 1-2 rings of activated Bacillus licheniformis strains from the test tube and inoculate them into the Erlenmeyer flask, and culture them for 20 hours at 32°C and 100 rpm after inoculation;
[0106] The medium formula of the triangular flask is: glucose 15g / L, soybean meal powder 16g / L, peptone 1g / L, NaCl 1g / L, KH 2 PO 4 0.3g / L, K 2 HPO 4 0.3g / L, pH7.0, sterilized at 121°C for 20 minutes:
[0107] c. Cultivation of 7 stra...
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