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Helicobacter pylori urease antibody IgM-IgG combination rapid test device and preparation method thereof

A technology for Helicobacter pylori and detection devices, which is applied in measuring devices, instruments, disease diagnosis, etc., can solve problems such as weak binding force and insufficient amount of protein adsorbed by NC membranes, and achieve the effects of reduced dosage, simple structure, and improved sensitivity

Active Publication Date: 2016-03-23
JILIN SHUANGZHENG MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a combined rapid detection device for Helicobacter pylori urease antibody IgM and IgG and a preparation method thereof, so as to solve the problems in the prior art that the amount of adsorbed protein on the NC membrane is insufficient and the binding force is not strong

Method used

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  • Helicobacter pylori urease antibody IgM-IgG combination rapid test device and preparation method thereof
  • Helicobacter pylori urease antibody IgM-IgG combination rapid test device and preparation method thereof
  • Helicobacter pylori urease antibody IgM-IgG combination rapid test device and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] (a) Preparation of colloidal gold by trisodium citrate reduction method

[0044] Quickly add gold chloride to heated 100ml purified water, after the solution boils again, quickly add trisodium citrate, gold chloride: trisodium citrate 1:0.5, continue to boil, observe that the color of the solution changes from yellow to black and then to purple , after finally turning into a stable wine red color, continue heating for 10 minutes by timing, and the colloidal gold particles are 20nm;

[0045] (b) Preparation of immunocolloidal gold

[0046] 1) Take 100ml of 20nm colloidal gold solution, add 140μl of pH regulator, mix well; let stand for 5min;

[0047] 2) In the 20nm colloidal gold solution, add Helicobacter pylori urease B antigen at a ratio of 14 μg per milliliter of colloidal gold solution, a total of 1.4 mg, mix well; let stand for 5 minutes;

[0048] 3) Add 0.4 ml of colloidal gold stabilizer according to the proportion of 0.4%, mix well, and let stand for 5 minutes...

Embodiment 2

[0066] (a) Preparation of colloidal gold by trisodium citrate reduction method

[0067]Quickly add gold chloride to heated 100ml purified water, after the solution boils again, quickly add trisodium citrate, gold chloride: trisodium citrate 1:1, continue to boil, observe the color of the solution from yellow to black and then purple , after finally turning into a stable wine red color, continue heating for 10 minutes by timing, and the colloidal gold particles are 40nm;

[0068] (b) Preparation of immunocolloidal gold

[0069] 1) Take 100ml of 40nm colloidal gold solution, add 140μl of pH regulator, mix well; let stand for 5min;

[0070] 2) In the 40nm colloidal gold solution, add Helicobacter pylori urease B antigen at a ratio of 14 μg per milliliter of colloidal gold solution, a total of 1.4 mg, mix well; let stand for 5 minutes;

[0071] 3) Add 0.4 ml of colloidal gold stabilizer according to the proportion of 0.4%, mix well, and let stand for 5 minutes;

[0072] 4) Cent...

Embodiment 3

[0089] (a) Preparation of colloidal gold by trisodium citrate reduction method

[0090] Quickly add gold chloride into heated 100ml purified water, after the solution boils again, quickly add trisodium citrate, gold chloride: trisodium citrate 1:2, continue to boil, observe the color of the solution from yellow to black and then purple , after finally turning into a stable wine red color, continue heating for 10 minutes by timing, and the colloidal gold particles are 60nm;

[0091] (b) Preparation of immunocolloidal gold

[0092] 1) Take 100ml of 60nm colloidal gold solution, add 140μl of pH regulator, mix well; let stand for 5min;

[0093] 2) In the 20nm colloidal gold solution, add Helicobacter pylori urease B antigen at a ratio of 14 μg per milliliter of colloidal gold solution, a total of 1.4 mg, mix well; let stand for 5 minutes;

[0094] 3) Add 0.4 ml of colloidal gold stabilizer according to the proportion of 0.4%, mix well, and let stand for 5 minutes;

[0095] 4) C...

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Abstract

The invention relates to a helicobacter pylori urease antibody IgM-IgG combination rapid test device and a preparation method thereof, and belongs to the field of medical test equipment. The device is prepared by pasting solid-phase purified high-specificity anti-human IgM and IgG antibodies, a nitrocellulose film of a helicobacter pylori urease antibody, a glass fiber film adsorbing a colloidal gold labeled helicobacter pylori urease antigen, a sample pad, absorbent paper and other auxiliary materials together; a polylysine treatment solution is adopted for pretreatment of the nitrocellulose film; the anti-human IgM and IgG antibodies are combined with silicon dioxide nano-particles at first, and then the combination is adsorbed onto the pretreated nitrocellulose film; an appropriate gold sputtering buffer solution and sample pad treatment solution are prepared; therefore, on the basis that complete release of immune colloid gold is guaranteed, the reaction sensitivity is effectively improved, and the consumption of the immune colloid gold and the cost can be reduced under the same threshold condition; the device is high in test paper sensitivity, strong in specificity, simple and convenient to operate, lower in time consumption, and strong in practicability.

Description

technical field [0001] The invention relates to the field of medical detection equipment, in particular to a combined rapid detection device for Helicobacter pylori urease antibody IgM and IgG and a preparation method thereof, which can realize sensitive, specific and rapid detection of Helicobacter pylori urease antibody IgM and IgG. Background technique [0002] Helicobacter pylori (Hp) infection plays an important role in the pathogenesis of chronic gastritis, peptic ulcer, gastric cancer and other gastrointestinal diseases. After infection with Helicobacter pylori, most people have mild or asymptomatic symptoms. Without treatment or delayed treatment, the condition will be severe. Therefore, it is of great significance to study the early detection of Hp infection. According to the law of antibody production, serum IgM appears 2 to 4 weeks after Helicobacter pylori infection, and then IgG appears 6 to 8 weeks after infection. IgM usually disappears completely after 2 to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/544
CPCG01N21/78G01N33/5308G01N33/531G01N33/544G01N33/558G01N33/56911G01N33/56983G01N33/573G01N33/6881G01N33/6893G01N2333/085G01N2333/952G01N2800/107G01N2800/324
Inventor 杨小军李欣赵旻王智鼎
Owner JILIN SHUANGZHENG MEDICAL TECH CO LTD
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