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Immune colloidal gold reagent for detecting ciprofloxacin and preparation method thereof

A technology of ciprofloxacin and colloidal gold, which is applied in the direction of antibodies, measuring devices, instruments, etc., can solve the problems of complex operation process, long time required for detection, high detection cost, etc., and achieve simple production process, easy promotion and use, and detection fast effect

Inactive Publication Date: 2004-11-17
博顿生物检验技术(杭州)有限公司
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AI Technical Summary

Problems solved by technology

The disadvantages of thin-layer chromatography are: the operation process is complicated and takes a long time; operators need to undergo professional training; there are many interference factors affecting the analysis, and the result repeatability is poor
The disadvantages of high-performance liquid chromatography and color / mass spectrometry analysis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technicians are required to operate, Operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of the pretreatment methods used in order to obtain reliable analysis results; the required instruments and equipment are expensive, so it is difficult to use them in cities. And popular among small and medium-sized enterprises; high testing costs
The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; Realize single-serve detection

Method used

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Embodiment Construction

[0007] Adhere one end of the PVC backing to the sample pad, the binding pad in turn, the nitrocellulose membrane in the middle, and the other end to the absorbent pad. The binding pad is coated with anti-ciprofloxacin specific monoclonal antibody-colloidal gold marker. The nitrocellulose membrane was coated with ciprofloxacin-BSA conjugate and goat (rabbit) anti-mouse IgG.

[0008] Prepare according to the following steps: (1) prepare ciprofloxacin-BSA conjugate: mix ciprofloxacin and BSA in a ratio of 1:5~50 (mol / mol), so that ciprofloxacin and BSA form a stable Particles were purified to form ciprofloxacin-BSA conjugates; (2) Preparation of anti-ciprofloxacin-specific monoclonal antibodies: multiple immunizations with ciprofloxacin-BSA conjugates of Balb / c clones For mice, mouse splenocytes and myeloma cells are fused in vitro to form hybridoma cells, and positive hybridoma cell lines can be obtained through selective medium screening. A large amount of anti-ciprofloxacin-...

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Abstract

The invention is a kind of immune colloidal gold reagent for measuring the ciprofloaxcin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of ciprofloaxcin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by ciprofloaxcin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for the detection of ciprofloxacin mainly include thin-layer chromatography, high performance liquid chromatography, color / mass spectrometry analysis, and enzyme-linked immunosorbent assay. The disadvantages of thin-layer chromatography are: the operation process is complicated and takes a long time; the operators need to undergo professional training; there are many interference factors affecting the analysis, and the repeatability of the results is poor. The disadvantages of high-performance liquid chromatography and color / mass spectrometry analysis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technicians are required to...

Claims

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Application Information

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IPC IPC(8): A61K39/395G01N33/532G01N33/558G01N33/569
Inventor 张少恩
Owner 博顿生物检验技术(杭州)有限公司
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