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Process method for synchronously preparing oleuropein and olive total flavonoids from olive leaves

A technology of olive leaves and oleuropein, which is applied in the field of simultaneous preparation of high-content oleuropein and olive total flavonoids extracts, can solve the problems of high production cost, low extraction rate, and long production cycle, and achieve low production cost, The effect of simple process route

Inactive Publication Date: 2011-05-18
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has the disadvantages of low content of oleuropein and flavonoids in the obtained extract, low extraction rate, high production cost, and long production cycle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 20 g of dried olive leaves, put them in a 500 mL round bottom flask, add 240 mL of 60% methanol, reflux for 1 hour in a water bath at normal pressure, and pour out the extract. Add 200 mL of 60% methanol to the residue, reflux and extract at normal pressure for 1 hour, pour out the extract, and discard the residue. Combine the two extracts and filter. After the filtrate is concentrated under reduced pressure to recover methanol, add water and dilute the volume to 250 mL so that the concentration is equivalent to 0.08 Kg per liter of olive leaves. Mix thoroughly to obtain Intermediate Product I.

[0031] Weigh 2 parts of polyacrylate macroporous adsorption resin, each 7g, and wet them into two glass columns (1BV=4mL) with an inner diameter of 1.0cm and a column height of 12cm (diameter-to-height ratio 1:12). The columns are connected in series (I column, II column). After adding 95% ethanol to column I and column II, soaking for 24 hours, wash with 95% ethanol until...

Embodiment 2

[0033] Weigh 1 kg of dried olive leaves, put them in a multi-effect extraction tank, add 10L of 50% ethanol to extract for 1.5 hours, and pour out the extract. Add 8L of 50% ethanol to the residue for 1.5 hours, pour out the extract, and discard the residue. Combine the two extracts and filter. After the filtrate is evaporated by thin film to recover ethanol, add water to make the volume to 5L, make the concentration equal to 0.2Kg per liter of olive leaf, and mix well to obtain intermediate product I.

[0034] Weigh 2 parts of polyacrylate macroporous adsorption resin, 0.8Kg each, and wet them into two resin columns with an inner diameter of 5.0cm and a column height of 50cm (diameter-to-height ratio 1:10) (1BV=0.98L) , Two columns in series (I column, II column). After adding 95% ethanol to column I and column II, soaking for 24 hours, wash with 95% ethanol until 3 times the volume of water is added and no white turbidity appears in the washing liquid, and then wash with wate...

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PUM

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Abstract

The invention discloses a process method for synchronously preparing oleuropein and olive total flavonoids from olive leaves. Olive leaves serve as raw materials, and the method comprises the following steps of: performing reflux or multiple extraction, series adsorption through macroporous adsorption resin, and gradient desorption; and recovering an organic solvent in desorption solution, and performing spray drying to respectively obtain high-content extracts of the oleuropein and the olive total flavonoids. The invention has the characteristics that: the high-content extracts of the oleuropein and the olive total flavonoids can be synchronously prepared, the production cost is low, the method is environmentally-friendly, and the process is simple.

Description

Technical field [0001] The invention belongs to the field of natural product chemistry, and relates to a process method for synchronously preparing high-content oleuropein and olive flavonoid extracts from olive leaves. Background technique [0002] Olea europaea (Olea europaea L.) is a broad-leaved plant of the genus Oleaceae Oleaceae, also known as Olea europaea and Zitun fruit. There are 15 species in my country, mainly in Gansu, Yunnan, Sichuan, Guizhou and other places. Oleuropein and olive total flavonoids are one of the most important active ingredients in olive leaves, with antioxidant and antibacterial effects, and their extracts are widely used in functional foods and medicines. [0003] The main active ingredients in olive leaves are polyphenol compounds, mainly including seroid and flavonoids. Oleuropein (oleuropein) is the most representative compound in the seroiridoids. In addition to its antioxidant activity, it also has the effects of lowering blood pressure and a...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08C07G99/00
Inventor 邸多隆李辰王晓飞
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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