Preparation method of olive leaf extract
An olive leaf extract and olive leaf technology, applied in the field of preparation of olive leaf extract, can solve the problems of high cost and difficulty in realizing large-scale production, and achieve the effects of low cost, reduced energy consumption and high yield
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Embodiment 1
[0038] Embodiment 1: a kind of olive leaf extract, it is made by following method, comprises the steps:
[0039] (1) After soaking the olive leaves in salt water for 1 hour, dry them and grind them into 40-60 mesh fine powder;
[0040] (2) Add 5 times of water to the raw material, add cellulase:alkaline protease 1:3 compound enzyme, the amount of compound enzyme is 1% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter, Obtain enzymatic solution;
[0041] (3) The enzymatic hydrolysis solution is directly passed through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4~1:15, and it is eluted with 1-3 times the column volume of water and 1-2 times the column volume of 95% ethanol solution respectively. Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 6 times the column volume, and collect the eluate;
[0042] (4) After evaporating glacial acetic acid and ethanol in the eluent, m...
Embodiment 2
[0043] Embodiment 2: a kind of olive leaf extract, it is made by following method, comprises the steps:
[0044] (1) After soaking the olive leaves in salt water for 2 hours, dry them and grind them into fine powder;
[0045] (2) Add 3 times water to the raw material, add cellulase:alkaline protease compound enzyme, the amount of compound enzyme is 2% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter to obtain enzymolysis liquid;
[0046] (3) The enzymatic hydrolysis solution passes directly through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4 to 1:15, and is eluted with 1-3 column volumes of water and 1-2 column volumes of 95% ethanol solution respectively Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 10 times the column volume, and collect the eluate;
[0047] (4) After evaporating glacial acetic acid and ethanol in the eluent, microwave vacuum drying, temperatur...
Embodiment 3
[0048] Embodiment 3: a kind of olive leaf extract, it is made by following method, comprises the steps:
[0049] (1) After soaking the olive leaves in salt water for 1 hour, dry them and grind them into 50-60 mesh fine powder;
[0050] (2) Add 7 times of water to the raw material, add cellulase:alkaline protease 1:3 compound enzyme, the amount of compound enzyme is 1% by weight of the raw material, keep enzymolysis at 30-50°C for 0.5h, filter, Obtain enzymatic solution;
[0051] (3) The enzymatic hydrolysis solution passes directly through the neutral alumina column, and the diameter-to-height ratio of the resin column is 1:4 to 1:15, and is eluted with 1-3 column volumes of water and 1-2 column volumes of 95% ethanol solution respectively Remove impurities, elute with 0.2-0.5% glacial acetic acid-50% ethanol (1:1) solution of 8 times the column volume, and collect the eluate;
[0052] (4) After evaporating glacial acetic acid and ethanol in the eluent, microwave vacuum dryi...
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