Method for extracting syringin from ovate leaf holly bark

A technology of syringin and jiubing, which is applied in the field of supercritical CO2 extraction and polyamide resin separation and extraction of syringin, can solve the problem of syringin yield and purity, non-specific process, syringin Less research and other issues, to achieve the effect of high extract content, simplified impurity removal process, and low energy consumption

Inactive Publication Date: 2012-07-11
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the extraction of syringin by this method, such as "Joubing supercritical CO 2 GC-MS analysis of extracts", this method experiment does not involve the yield and purity of syringin, it is not a specific process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Rescue Bing herbs, crush them into 80 meshes, take 500g and put them into the extraction kettle, turn on the equipment and adjust the parameters: extraction temperature is 50°C, extraction pressure is 15MPa, CO 2 The flow rate is 20 L / h, the temperature of the separation tank I is 55° C., and the pressure is 9 MPa, and the temperature of the separation tank II is 40° C., and the pressure is 5 MPa. After reaching the above parameters, extract for 2 hours while analyzing the extract, and discard the extract. Then adjust the equipment parameters: the extraction temperature is 50°C, the extraction pressure is 45MPa, CO 2 The flow rate is 30 L / h, the temperature of the separation tank I is 45° C., and the pressure is 10 MPa, and the temperature of the separation tank II is 30° C., and the pressure is 5 MPa. After reaching the parameters, extract for 3 hours, analyze the extract, and obtain 25g of syringin extract, stir and dissolve with 200ml of 40% ethanol, filter, add the...

Embodiment 2

[0023] Rescue Bing herbs, crush them into 60 meshes, take 1kg and put them into the extraction kettle, turn on the equipment and adjust the parameters: the extraction temperature is 40°C, the extraction pressure is 18MPa, CO 2 The flow rate is 22L / h, the temperature of the separation tank I is 50°C, and the pressure is 7MPa, and the temperature of the separation tank II is 30°C, and the pressure is 5MPa. After reaching the above parameters, extract for 2 hours while analyzing the extract, and discard the extract. Then adjust the equipment parameters: the extraction temperature is 65°C, the extraction pressure is 45MPa, CO 2 The flow rate is 40L / h, the temperature of the separation tank I is 45°C, and the pressure is 7MPa, and the temperature of the separation tank II is 45°C, and the pressure is 5MPa. After reaching the parameters, extract for 4 hours, analyze the extract, and obtain 46g of syringin extract, stir and dissolve with 500ml of 20% ethanol, filter, add the filtrat...

Embodiment 3

[0025] Rescue Bing herbs, crush them into 40 meshes, take 2kg and put them into the extraction kettle, turn on the equipment and adjust the parameters: extraction temperature is 45°C, extraction pressure is 25MPa, CO 2 The flow rate is 20 L / h, the temperature of the separation tank I is 50° C., and the pressure is 6 MPa, and the temperature of the separation tank II is 35° C., and the pressure is 5 MPa. After reaching the above parameters, extract for 2 hours while analyzing the extract, and discard the extract. Then adjust the equipment parameters: the extraction temperature is 60°C, the extraction pressure is 40MPa, CO 2 The flow rate is 45L / h, the temperature of the separation tank I is 45°C, and the pressure is 8MPa, and the temperature of the separation tank II is 35°C, and the pressure is 5MPa. After reaching the parameters, extract for 3 hours, analyze the extract, and obtain 105 g of syringin extract, stir and dissolve with 900 ml of 30% ethanol, filter, add the filtr...

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PUM

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Abstract

The invention relates to a method for extracting syringin from ovate leaf holly bark. The method comprises the following steps of: crushing the ovate leaf holly bark medicine into ovate leaf holly bark with 40-80 meshes, adding the crushed ovate leaf holly bark into an extraction kettle, collecting syringin extract by using supercritical CO2 gradient extraction, dissolving the syringin extract by using 20-40% alcohol solution, filtering, adding a polyamide resin column into filtrate to adsorb impurities, carrying out concentration under reduced pressure on over-column liquid, standing for crystallization, filtering crystals, dissolving the crystals in hot water, adding activated carbon for decolorization, carrying out concentration under reduced pressure on decolorized liquid, and then standing for crystallization, drying crystals to obtain a product. The method has the advantages of low energy consumption and less pollution and is an environmentally-friendly extraction and separation method.

Description

Technical field: [0001] The invention relates to a method for extracting syringin from jiubing, in particular to a method for applying supercritical CO 2 Method for extraction and polyamide resin separation and extraction of syringin. Background technique: [0002] Syringin, also known as syringin and rescue glycoside A, is a phenylpropanoid compound. Molecular formula C 17 h 24 o 9 , molecular weight 372.36, slightly soluble in cold water, slightly soluble in acetone, soluble in hot water, methanol, ethanol, almost insoluble in ether, benzene, chloroform. [0003] Syringin has anti-inflammatory, analgesic and anti-tumor effects, and is often used in hemostasis and anti-hepatotoxicity. [0004] There are many extraction techniques for syringin, which are generally extracted and purified from Acanthopanax, Saussurea sinensis, and Jiubing. Most of them are extracted with low-carbon alcohol or acetone, and most of them are purified by extraction or macroporous resin adsorp...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCY02P20/54
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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