Tissue culture and rapid propagation technology for Clematis 'Arabella'

Abstract

The invention discloses a tissue culture and rapid propagation technology for Clematis 'Arabella', which belongs to the technical field of tissue culture and rapid propagation of the Clematis 'Arabella'. In the technology, a regeneration system of the Clematis 'Arabella' is established by taking a stem segment, which is provided with buds, of a new branch in the current year as an explant. The research discovers that: the optimal culture medium for axillary bud induction comprises a murashige and skoog (MS) culture medium, 2,4-dichlorophenoxyacetic acid at the concentration of 1.0mg / L, indolebutyric acid at the concentration of 1.0mg / L and sucrose at the concentration of 30g / L, and the differentiation rate reaches 100 percent; the optimal culture medium for adventitious bud proliferation comprises an MS culture medium, 6-benzyladenine at the concentration of 0.5mg / L, indolebutyric acid at the concentration of 0.1mg / L and sucrose at the concentration of 30g / L, and the proliferation coefficient is 3.98; and an appropriate rooting culture medium comprises a 1 / 2MS culture medium and naphthylacetic acid at the concentration of 0.04mg / L, and the rooting rate reaches 89.3 percent. Through 5 to 7 days of bottle-opening seedling hardening, pearlite and peat in a ratio of 1:3 are taken as matrices, and after 20 days, the investigation discovers that the transplant survival rate of seedlings reaches 90 percent. The technology can be applied to the efficient and rapid propagation of the Clematis 'Arabella', and can realize the industrial breeding of the Clematis 'Arabella'.

Description

1. Technical field [0001] The invention relates to the tissue culture rapid propagation technology of clematis 'Arabella'. 2. Background technology [0002] Clematis 'Arabella' (Clematis 'Arabella') belongs to Ranunculaceae (Ranunculaceae) Clematis L., is a hybrid of Clematis 'Integrifolia' and Clematis 'Lanujinosa', native to from the UK. This variety has various flower types and inflorescences, rich flower colors, long flowering period, wide range of horticultural uses, and high ornamental value. As a vertical greening material, 'Arabella' is not only used in the field of gardens, but also widely used in potted plants, cut flowers, etc. [0003] Clematis 'Arabella' has high ornamental value and wide application prospects, but its promotion is slow because of its difficulty in seed propagation. At present, my country has not yet developed its rapid propagation technology, and this variety still needs to be imported in large quantities from abroad, which limits the promot...

AI Technical Summary

Problems solved by technology

[0004] There are many varieties of clematis, but so far there are not many studies on the tissue culture of clematis. Zeren Wangmo used the seedlings grown from the seeds of clematis to produce adventitious buds and roots; Zhang Qixiang found out the ' The best explant of Multi-Blue' is the shoot tip; while Zhang Tao induced the callus of Clematis double petals, but did not induce adventitious buds

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