Tissue culture method for increasing survival rate of psidium guajava L.

Abstract

The invention relates to the field of plant tissue culture and discloses a tissue culture method for increasing the survival rate of psidium guajava L.. The tissue culture method comprises the following first step of disinfection of a culture room, the second step of selection of explants, the third step of sterilization of the explants, the fourth step of initial induction culture, the fifth step of subculture, and the sixth step of seedling hardening. According to the method, the explants are disinfected with plant treating liquid, and thus the survival rate of the explants in the tissue culture process is increased; meanwhile, a commonly-used MS culture medium is replaced with cassava alcohol waste liquid, and thus the tissue culture cost can be effectively reduced. The tissue culture method can be applied to various explants of psidium guajava L., the surfaces of the explants can be effectively disinfected without disoperation to the explants, and meanwhile the production cost can be effectively reduced.

Description

【Technical field】 [0001] The invention relates to the field of plant tissue culture, in particular to a tissue culture method for improving the survival rate of pearl guava. 【Background technique】 [0002] Guava is a plant of the genus Guava in the family Myrtaceae. It is native to tropical America. Now it is widely cultivated in many tropical and subtropical regions. In my country, it is mainly distributed in Hainan, Guangxi, Guangdong, Fujian and Taiwan. The growth of guava fruit trees The requirements on the environment are very strict. The current seedling cultivation methods of guava fruit trees are mostly grafting cultivation. The grafting cultivation period is usually 1-2 years to obtain pearl guava seedlings. The cultivation period of grafting cultivation is long and the cultivation method is complicated. At present, there are also Some scholars begin to study the tissue culture method of pearl guava fruit tree, but the explant of pearl guava fruit tree is easy to infe...

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Problems solved by technology

[0002] Guava is a plant of the genus Guava in the family Myrtaceae. It is native to tropical America. Now it is widely cultivated in many tropical and subtropical regions. In my country, it is mainly distributed in Hainan, Guangxi, Guangdong, Fujian and Taiwan. The growth of guava fruit trees The requirements on the environment are very strict. The current seedling cultivation methods of guava fruit trees are mostly grafting cultivation. The grafting cultivation period is usually 1-2 years to obtain pearl guava seedlings. The cultivation period of grafting cultivation is long and the cultivation method is complicated. At present, there are also Some scholars have begun to study the tissue culture method of the pearl guava fruit tree, but the explants of the pearl guava fruit tree are easy to be infected with bacteria, cause browning, and are sensitive to sterilizers, so it is not easy to cultivate pearl guava fruit trees according to conventional methods.

[0003] At present, in the process of plant tissue culture, the commonly used sterilizing agent in my country is mainly mercury chloride. A lot of research on the toxic effect of mercury has been carried out at home and abroad, and it has been confirmed that mercury is important for every growth process of cells. Therefore, the explants sterilized by mercury chloride need to completely remove the residual Hg+, so as to eliminate the damage to the explants caused by the trace amount of residual mercury chloride. The explants of pearl guava fruit trees are not easy to carry out tissue culture and reproduction. It is necessary to provide an ideal tissue culture method in the tissue culture process of pearl guava, which can not only be applied to various explants of pearl guava, but also effectively disinfect the surface of the explants without sterilizing the explants. Infringement, but also can effectively reduce production costs

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