86results about How to "Achieve multiplication" patented technology

The invention belongs to the technical field of the nuclear reactor engineering, particularly to the water-cooling two-region multiplication nuclear reactor core and the nuclear reactor adopting the same, wherein the water-cooling two-region multiplication nuclear reactor core includes a fast neutron energy spectrum area adopting the coating grain sheath fuel assembly and a thermal neutron energy spectrum area adopting the dense rod bundle fuel assembly or the coating grain sheath fuel assembly. The water-cooling two-region multiplication nuclear reactor core of the invention adopts the coating grain sheath fuel assembly, which breaks through the restrain of the stainless steel shell to the outlet scream temperature, realizes the higher coolant outlet temperature, meanwhile the fast neutron core is leaked to the thermal neutron energy spectrum core constituted by the rod bundle fuel assembly dense boom at the subcritical to be multiplied.

The invention discloses a bioreactor in vascular tissue engineering, which comprises a hemodynamics simulating device, a 3D bracket and culture chambers. The hemodynamics simulating device is a pulsating flow control device; the culture chambers are serially connected with each other in the pulsating flow control device and include hermetically matched rotary devices and a sealed cavity; the rotary device includes a rotary rod having the 3D bracket fixed thereon and a drive device for rotating the rotary rod; and the rotary rod is located in the sealed cavity. The bioreactor can provide a pulsating flow simulating environment of human body facilitating growth, proliferation and differentiation of cells by adopting the pulsating flow control device for providing the dynamic effect simulating human heart. In the culture chambers, cells are inoculated in the internally-rotating 3D bracket, and secondary cell inoculation can be further executed to achieve convenient taking and dropping of cells, so that the cells can uniformly adhered on the 3D bracket, thus obviating the contamination of the reactor connected with the 3D bracket, on which the cells are inoculated through rotation.

The invention belongs to the field of biomedical materials, and particularly relates to photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and a preparation method thereof. The photo-curing hydrogel is prepared by taking modified high-polymer materials with the photo-curing performance as raw materials and are divided into multiple different areas by straight-stripe-shaped or net-stripe-shaped or annular-stripe-shaped micro-pattern structures, the hardness of the photo-curing hydrogel in the same area is same, the hardness of the photo-curing hydrogel in the different areas is same or different, and the overall photo-curing hydrogel at least has two kinds of the hardness. According to the photo-curing hydrogel for multi-cell sorting and stem cell area-selecting differentiation and the preparation method thereof, on one hand, the elasticity moduli of all the areas of the photo-curing hydrogel are different by controlling the ultraviolet radiation curing degree; on the other hand, all the areas with the different elasticity moduli present the micro-pattern structures on the photo-curing hydrogel, selective adhesion, proliferation and differentiation of cells on the photo-curing hydrogel can be achieved by combining the elasticity moduli with the micro-pattern structures, and therefore controllable cell sorting or stem cell area-selecting differentiation can be achieved.

A twig tissue culture propagation method for cinnamomum camphora (L.) presl is disclosed. The method is characterized in that the method includes explant selecting, explant processing, primary shoot induction culture, multiplication culture, strong-shoot culture and rooting culture, annual twigs robust in growth and at the periphery of the crown of the cinnamomum camphora (L.) presl are collected and adopted as explants, the explants after being trimmed, sterilized and disinfected are inoculated into a primary shoot induction culture medium to obtain primary buds, the primary buds are inoculated to a multiplication culture medium and subjected to 4-5 cycles of multiplication culture to form cluster buds, the cluster buds are inoculated to a strong-shoot culture medium and cultured, and a single shoot the height of which is not less than 2 cm is inserted into a rooting culture medium and subjected to root induction. The method is simple and convenient in operation, short in culture period, high in subcultured shoot yield and good in subcultured shoot quality. The multiplication constant is 5 or above. The method meets technique requirements on industrialization of tissue-cultured seedlings, and has good economic benefit, ecological benefit and social benefit.

The invention discloses an analog circuit for realizing the characteristics of a memory inductor. The circuit comprises a memory inductor equivalent circuit and a magnetic flux generating circuit; the magnetic flux generating circuit is composed of an integrated operational amplifier U1 and is adopted as the input of the memory inductor equivalent circuit, wherein the integrated operational amplifier U1 is used for realizing a first integrator and a reverse amplifier; the memory inductor equivalent circuit includes an integrated operational amplifier U1 and a multiplier U2, wherein the integrated operational amplifier U1 is used for realizing a second integrator and an adder; the reverse amplifier and the second integrator are connected with the multiplier, so that signal multiplication can be realized, and output acts on the adder, so that final memory inductor current quantity can be obtained. The analog circuit only includes one integrated operational amplifier chip and one multiplier, so that the analog circuit has structural simplicity. The analog circuit of the invention can replace an actual memory inductor when the actual memory inductor cannot be obtained at present and in the further, so that circuit design, experiments and application related to the memory inductor can be realized. The analog circuit is of great practical significance for the characteristic and application research of the memory inductor.

The invention relates to a method for detoxifying and reproducing helianthus tuberosus quickly. The method comprises the following steps of: disinfecting young axillary buds or stem tips serving as explants, transferring the young axillary buds or stem tips into a bud induction culture medium, transferring the buds into a rooting culture medium, and performing refining seedlings and transplanting on a complete plant, wherein the bud induction culture medium is improved on the basis of a Murashige and Skoog (MS) culture medium, 0.1 mg.L<-1> of naphthyl acetic acid (NAA) and 0.5 mg.L<-1> of 6-butyl acrylate (BA) are added, so that adventitious buds which are induced are more, callus is small, and the problems of vitrification and water stain are solved; and obtained seedlings are robust and vigorous in growth vigor and can be transferred into the rooting culture medium directly, the rooting rate is 100 percent, and the survival rate of test-tube seedlings is over 90 percent. By the method, the effect of detoxifying the helianthus tuberosus seedlings can be achieved, the reproduction efficiency is improved, the production period is shortened, and an effective path is provided for the large-scale seedling raising of the helianthus tuberosus.

The invention discloses an artificial algal reef and a manufacturing method thereof, and relates to the artificial algal reef and a preparation method thereof. The artificial algal reef comprises an algal reef main body made from reinforcements and concrete; vegetable gum grains are arranged on the algal reef main body, and have the grain size of 1 to 60 mm; and the weight of the vegetable gum grains is 0.01% to 0.6% of the total weight of the artificial algal reef. The preparation method of the artificial algal reef comprises the following steps: 2 to 6 parts of cement, 3 to 10 parts of sand, 8 to 12 parts of stone and 0.001 to 0.008 part of vegetable gun grains are selected through proportioning in part by weight, wherein the grain sizes of the vegetable gum grains are 1 to 10 mm; the algal reef is prepared from cement, sand, stones and a reinforcement cage; and the vegetable gum grains are added before hardening. When the artificial algal reef is used, vegetable gum is dissolved in the water, a microcellular structure is formed on the surface of a reef, the roughness of the surface of the reef is increased, the adhesion possibility of algae spores is improved, a sessile substrate suitable for the adhesion and the growth of the algae spores is provided, and habitats for foraging, breeding, growing and the like are provided for aquatic organisms such as fishes.

The present invention relates to a method capable of adopting isolated induction process to obtain winter jujube anther callus. It is characterized by that said invention utilizes tissue culture of winter jujube anther to obtain its callus. Said method includes the following several steps: explant collection, disinfection, in initial induction and enrichment culture. Besides, said invention also provides the concrete requirements of the above-mentioned every step and its concrete operation method.

The invention discloses a method for establishing a racomitrium japonicum gametophyte regeneration system. The method provided by the invention comprises the following steps of shearing racomitrium japonicum subjected to appropriate culture into small racomitrium japonicum segments from top to bottom, carrying out surface disinfection, inoculating a conventional MS culture medium with the sterilized small racomitrium japonicum segments, carrying out culture, carrying out screening to obtain aseptic explants, transferring the aseptic explants to a medium comprising a conventional MS medium and30g / L of cane sugar, inducing gametophytes to produce protonemata, transferring the protonemata to a propagation medium, carrying out culture for 50 days, transferring the protonemata obtained by theprevious step to a medium for producing gametophytes by induction, and carrying out culture for 70 days. The method for establishing a racomitrium japonicum gametophyte regeneration system is a novelmethod, has simple processes and a low cost, can realize propagation of a mass of racomitrium japonicum gametophytes in a short time, and guarantees the success of follow-up experiments.

The invention discloses a fermentation film containing rich lactobacillus plantarum and a preparation method thereof, and belongs to the technical field of microbes and the technical field of fermentation. According to the method, the lactobacillus plantarum is inoculated into raw material milk added with proliferation factor for fermentation so as to obtain the fermentation milk. By using the method provided by the invention, the proliferation and the fermentation of the lactobacillus plantarum in the cow milk fermentation system are successfully realized; in addition, the fermentation time of the lactobacillus plantarum in the cow milk fermentation system can be greatly shortened. When the method provided by the invention is used for preparing the fermentation milk, the viable count of the lactobacillus plantarum in the fermentation milk can reach 1.2*10<9>CFU / g after the fermentation for 10 to 12h; in addition, the fermentation milk is prepared by using the method; the problem of poor flavor (beany flavor or bitter taste) of products due to the use of yeast powder, soybean protein, soy peptone or hydrolyzed soybean protein as accelerators for accelerating the fermentation of thelactobacillus plantarum in the cow milk is solved; the large-scale application of the lactobacillus plantarum in the fermented milk product is greatly promoted.

The present disclosure discloses an integrated micro / nanogenerator and a method of fabricating the same The integrated micro / nanogenerator has a structure comprising a conducting layer, a PET layer, a PDMS layer, a micro-nano hierarchical PDMS array and a metal film layer, the conducting layer being manufactured on a surface of the PET layer, the PET layer being made of polyethylene terephthalate; the PDMS layer being made of polydimethylsiloxane, and the micro-nano hierarchical PDMS array being manufactured on a surface of the PDMS layer. The method comprises steps of: 1) fabricating a micro-scale structure on a substrate through a combination of lithography and chemical etching or physical etching; 2) fabricating a nano-scale structure with high density and high depth-to-width ratio directly on a surface of the micro-scale structure through a mask-free optimized deep reactive ion etching process; 3) using a PDMS casting film transfer process by adjusting and controlling process parameters, by means of using the mold of mirco-nano hierarchical array structure as a template; 4) fabricating a conducting layer on a surface of the PET layer by using an evaporation or sputtering or chemical vapor deposition process; 5) bonding the PDMS layer and the PET layer through high temperature bonding or normal temperature physical pressing; and 6) assembling in sequence and packaging the bonded structure obtained in step 5), the metal film layer, and another bonded structure obtained in step 5).

The invention discloses a clinical-grade autogenous bronchial basal layer cell, a transfusion preparation and a preparation technology in the technical filed of regeneration medicine. The preparationtechnology specifically includes the following steps: taking in-vitro active bronchial brushing tissue to perform digestive treatment, and collecting cells after digestion is finished; performing planking culture on the digested cells by using a culture plate coated with trophoblastic cells, collecting the cells and using the culture plate coated with the trophoblastic cells to perform amplification culture, and performing subculturing when the cells are grown to 50%-90% of the surface area of the culture plate; and performing digestion and collecting adherent cells when the subcultured cellsare grown to 85%-95% of the surface area of the culture dish, and then performing washing. The clinical-grade cells are firstly determined to be suitable for lung injury repair; through the selectionof proper preparation technology, the industrialized preparation of the cells can be realized, and bronchial basal layer cells that meet the quantity and quality of clinical cell treatment can be obtained within a short time; and the bronchial basal layer cells prepared by the method can stably differentiate when entering the focus, so that the obvious repairing of the lungs can be realized.

The invention provides a method for promoting directional differentiation and proliferation of mesenchymal stem cells towards neural precursor cells. The method comprises the following steps: firstly, embedding the mesenchymal stem cells in calcium alginate microbeads which are prepared with the presence of a calcium chloride solution and contains gelatin microspheres and chondroitin sulfate; then, adding a neural induction differentiation medium, and cultivating in a rotary reactor, so as to promote the in vitro directional differentiation and proliferation of the mesenchymal stem cells towards the neural precursor cells; and after differentiation, dissolving the calcium alginate microbeads by virtue of a sodium citrate solution, so that differentiated cells are obtained. The method disclosed by the invention is simple to operate and low in cost; by virtue of the gelatin microspheres, a necessary growth space is provided for internal cells of a stent; by virtue of the chondroitin, the biochemical functions of a neural tissue extracellular matrix can be simulated; by virtue of the calcium alginate microbeads, three-dimensional support, which is similar to the extracellular matrix, is provided; and by virtue of the rotary reactor, a necessary micro-gravity environment is provided, so that mass transfer is further enhanced and proliferation of the cells is achieved.

The invention discloses a method building a suspension cell line of hevea brasiliensis, which comprises a preparing process of explant, an induction process of embryogenic callus, a continuing culture process of embryogenic callus, and a suspended culture process of embryogenic callus. The invention has the advantages of simple technological process, low production cost, rapid and steady proliferation and long term continuing culture of the built suspension cell line; solving the problem of the influence to the obtaining of the general hevea brasiliensis embryogenic callus from the season and climate, scale proliferation, and the labour and material resource consumption; providing an important foundation for the large-scale commercialization, factory production and large-scale field planting of the hevea brasiliensis self-rooted clone; providing new way and condition for creating new excellent germplasm resources of hevea brasiliensis utilizing the biotechnology methods of somatic hybridization, genetic transformation and mutant induction; laying a foundation for the research and development of hevea brasiliensis bioreactor.

The invention discloses a tissue engineering 3D stent based on bean curd as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly pre-freezing the bean curd, and then carrying out freeze-drying treatment to obtain the tissue engineering 3D stent based on bean curd. A loose and porous structure is formed in the tissue engineering 3D stent prepared by taking the bean curd as the raw material; various and obvious pore channels are communicated with each other; the requirement of the cell growth on the stent material structure is met; after the prepared 3D stent is simply treated, the 3D stent can be directly applied to the tissue engineering field; the cells achieve effective adhesion, growth and proliferation on the surface of the3D stent; the 3D stent has an obvious promotion effect on the growth of the cells; meanwhile, the product is stable in structure, high in biocompatibility and excellent in mechanical property; in addition, the tissue engineering 3D stent is simple to operate and high in repeatability; the condition is easy to implement; the prepared product is reliable in performance and has broad application prospects in the tissue engineering field.

The invention discloses a method for efficiently treating plant protein wastewater, and belongs to the field of protein wastewater treatment. The method includes 'multistage pre-treatment, efficient anaerobic, anoxia and aerobic' combined technologies, so that efficient anaerobic treatment, stable running and recycling can be carried out on the plant protein wastewater. A 'multistage pre-treatment technology' mainly includes physiochemical per-treatment and biochemical pre-treatment; suspended particles in the plant protein wastewater can be efficiently intercepted by the aid of the physiochemical pre-treatment, and bean pulp resources in the plant protein wastewater can be recycled by the aid of the physiochemical pre-treatment; protein can be destructed by the aid of the biochemical pre-treatment, calcium ions and phosphate radicals can be intercepted by the aid of the biochemical pre-treatment, and anaerobic disturbance influence factors can be eliminated; the pH (potential of hydrogen) and the temperature of inlet water can be reasonably controlled by efficient anaerobic units, accordingly, anaerobic systems can assuredly efficiently and stably run, and the volume load of the anaerobic systems can reach 25 kg / (m<3>.d). Compared with the traditional processes and technologies for plant protein wastewater, the technical method has the advantages of high efficiency, stability, high resource utilization rate and the like. Besides, the plant protein wastewater can be efficiently treated by the aid of the method.

The invention relates to a tissue culture and rapid propagation method of micro tubers of rhizoma bletillae. The method comprises the following steps: (1) selecting rhizoma bletillae fruits; (2) performing aseptic seeding; (3) culturing to enable seed embryo germination; (4) performing differentiation culture on buds; and (5) rooting and performing induced culture on the micro tubers. According to the method, different illumination intensity and culture temperature are provided in different growing stages, so that the seed embryo of rhizoma bletillae can efficiently germinate, and leaf primordium is timely differentiated to form the buds after germination; the rooting and the forming of the tubers are induced with the same culture medium, so that the seed germination, the leaf bud differentiation, the rooting, seedling growing and the inducing of the micro tubers are synchronously performed, and as a result, the inducing rate is high; the quality of the seeds and the seedlings is high; the transplanting survival rate is high; the high-quality production of the seeds and the seedlings of rhizoma bletillae is achieved; the wild resource can be effectively protected and rescued; and moreover, the demand on mass production is met.

The invention discloses a tissue culture and rapid propagation method suitable for tung trees. The method comprises the following processes: selecting explants, treating the explants, carrying out primary bud induction culture, carrying out propagation culture, culturing strong buds and carrying out rooting culture; the method comprises the concrete steps of collecting semi-lignified tung tree twigs, which grow robustly in the very year, as the explants, carrying out pruning and sterilizing treatment on the explants, and then inoculating an initial bud induction medium with the explants so as to obtain initial buds; inoculating a propagation medium with the initial buds, and carrying out the propagation culture for 3-4 cycles to form multiple shoots; inoculating a strong bud culture medium with the multiple shoots for culturing; finally, inserting a single bud with the height of more than or equal to 2cm into a rooting medium for inducing a root. After the tissue culture and rapid propagation method is used, the seedling raising period of the tung trees is shortened, and seedling raising materials are saved; the method solves the problems that the traditional seedling raising method needs more seedling raising materials, is long in cycle, and the like, greatly lowers the production cost, increases the production efficiency, and has better economic benefit, social benefit and ecological benefit.

The invention belongs to the technical field of medical treatment and laboratory equipment, and relates to a novel cell culture vessel related to cell culture. The cell culture vessel mainly consists of a vessel frame, protecting films, end covers, press frames and other components, wherein a plurality of ports can be arranged on the two side surfaces of the vessel frame, the protecting films are pressed on the upper and lower two end surfaces of the vessel frame through the press frames, and then the end covers are screwed on the ports of the vessel frame to form a closed cavity in the whole frame body; also, the ports can be connected with external instruments and equipment to realize continuous perfusion of a culture solution and cell gathering during cell culture. The cell culture vessel is characterized in that cell proliferation and cell differentiation can be realized without the need of filling gaseous substances such as O2, CO2 and N2 required by cell culture, and cell sorting and culture can be carried out in the same culture vessel. The cell culture vessel has a simple structure and convenient use, and the inner space of the culture vessel can realize proliferation and co-culture of various types of cells.

The present disclosure discloses an integrated micro / nanogenerator and a method of fabricating the same The integrated micro / nanogenerator has a structure comprising a conducting layer, a PET layer, a PDMS layer, a micro-nano hierarchical PDMS array and a metal film layer, the conducting layer being manufactured on a surface of the PET layer, the PET layer being made of polyethylene terephthalate; the PDMS layer being made of polydimethylsiloxane, and the micro-nano hierarchical PDMS array being manufactured on a surface of the PDMS layer. The method comprises steps of: 1) fabricating a micro-scale structure on a substrate through a combination of lithography and chemical etching or physical etching; 2) fabricating a nano-scale structure with high density and high depth-to-width ratio directly on a surface of the micro-scale structure through a mask-free optimized deep reactive ion etching process; 3) using a PDMS casting film transfer process by adjusting and controlling process parameters, by means of using the mold of mirco-nano hierarchical array structure as a template; 4) fabricating a conducting layer on a surface of the PET layer by using an evaporation or sputtering or chemical vapor deposition process; 5) bonding the PDMS layer and the PET layer through high temperature bonding or normal temperature physical pressing; and 6) assembling in sequence and packaging the bonded structure obtained in step 5), the metal film layer, and another bonded structure obtained in step 5).

The invention discloses a cinnamomum camphora somatic embryogenesis and tissue culture method, and belongs to the technical field of plant tissue culture. The method comprises the following steps: collecting immature fruits as explants, sterilizing, cutting the fruits, and taking out immature zygotic embryos; carrying out induction culture on the somatic embryos and the embryogenic calluses, carrying out proliferation, maturation and germination on the somatic embryos and the embryogenic calluses, and carrying out breeding culture and rooting culture on somatic embryo seedlings to obtain seedlings; realizing the proliferation, maturation and germination of the somatic embryos and the embryogenic calluses by the transformation of the cinnamomum camphora somatic embryos and the embryogenic calluses in the same stage through a general culture medium for proliferation, maturation and germination of somatic embryos to generate somatic embryo seedlings. According to the method, the general culture medium for proliferation, maturation and germination of somatic embryos can realize conversion of camphor tree embryogenic calluses into somatic embryo seedlings within 3-4 months, a large number of rooting camphor tree somatic embryo seedlings can be obtained within about 6 months, and the method has the advantages of omitting operation procedures, being high in efficiency and being simple and convenient to use.

The invention discloses a double-protein fermented milk beverage rich in active Lactobacillus plantarum and a preparation method thereof, and belongs to the technical field of fermentation engineering. The preparation method includes the following steps: inoculating Lactobacillus plantarum into double-protein base material components with the addition of proliferating factors and inorganic salts for fermentation to obtain fermented milk, and then adding stabilizers, sweeteners and water into the fermented milk for blending to obtain the fermented milk beverage; the preparation method can successfully realize the proliferation and fermentation of the Lactobacillus plantarum in the double-protein base material, and can greatly shorten the fermentation time of the Lactobacillus plantarum in the double-protein base material; the fermented milk beverage obtained by the preparation method is high in probiotic content, and the viable count can reach 5.0 x 10 8(superscript) cfu / mL or above; regarding the fermented milk beverage, the product system is stable, the protein content meets the requirements of national standards, sourness and sweetness are palatable, and the cost can be controlled; and the fermented milk beverage obtained by the preparation method is performed with low temperature storage at 0-10 DEG C for 21 days, and the viable count can be stabilized at 3.0 x 10 7(superscript) cfu / mL or above.

The invention relates to an open tissue culture method of polygonatum cyrtonema. According to the method, under the action of the bacteriostatic action of a YJ104 bacteriostatic agent and by addition of the YJ104 bacteriostatic agent into a polygonatum cyrtonema tissue culture medium, the growth and propagation of fungi and bacteria in the polygonatum cyrtonema tissue culture process are inhibited, so that the proliferation and rooting culture of the polygonatum cyrtonema under the bacterial condition are realized. The method is simple to operate, time-saving and labor-saving, greatly reduces the cost, and lays an important foundation for the development of an industrialized seedling production technology of the polygonatum cyrtonema.

The invention discloses a tissue culture propagation method for anthurium andreanum. The method includes explant treatment, induction culturing, proliferation culturing and rooting culturing; daughterplants of anthurium andreanum are selected as explants; the explants are inoculated into an induction medium to perform cultivation after being washed and treated through sterilization and disinfection, so that anthurium andreanum calluses can be obtained; then, the anthurium andreanum calluses are transferred to a proliferation medium to perform proliferation culturing; and differentiated multiple shoots with lengths of 1-2 cm are transferred to a rooting medium to perform cultivation, so that rooting anthurium andreanum can be obtained, and rooting rates can reach 100%. Through the induction, proliferation and rooting on the shoot tips of the anthurium andreanum, anthurium andreanum tissue culture seedlings can be rapidly obtained; the method is fast in rooting, so that the tissue culture time of the anthurium andreanum can be shortened, and therefore, low costs can be achieved; and the survival, differentiation and rooting rates of the anthurium andreanum can be enhanced, so that the method can have good economic benefits, social benefits and ecological benefits.

The invention relates to the field of immunology, in particular to pharmaceutical composition for in vitro amplification of gamma delta T cells and an amplification method of the pharmaceutical composition and provides pharmaceutical composition for in vitro amplification of cytotoxic gamma delta T-lymphocytes and an amplification method of the pharmaceutical composition to overcome the defects ofcomplexity of existing methods for acquiring gamma delta T cells. The pharmaceutical composition comprises ZOL (zoledronate) with the concentration of 1-5 mu mol / L and LEN (lenalidomide) with the concentration of 0.1-1.2 mu mol / L. The provided LEN and ZOL pharmaceutical composition can realize proliferation and activation of cytotoxic gamma delta T cells. Compared with existing methods for acquiring gamma delta T cells, the provided amplification method comprises simple procedures, injection of IL-2 is not required, the clinical applicability is high and the cost is lower.

The invention discloses a method for eliminating organic pollutant residues in soil by utilizing genetically engineering modified methane-oxidizing bacteria. According to a genetically engineered bacterium, the methane-oxidizing bacteria are used as host, and an organic pollutant degrading enzyme gene is introduced through a genetically engineering means. The gene coding the pollutant degrading enzyme is introduced into the strains by means of the genetically engineering means and is functionally expressed through optical transcription and translation elements, so that the performance of degrading target pollutants is given to the strains. According to the method, the gene coding the pollutant degrading enzyme is introduced into methane-oxidizing bacteria by utilizing the genetically engineering means, and the performance of degrading organic pollutants is given to the strains; and a certain amount of engineering bacteria are mixed into polluted soil, and survival and reproduction of the engineering bacteria are promoted by providing sufficient methane, so that pollutants can be effectively degraded.

The invention discloses a polypeptide self-assembled hydrogel stent and application thereof. A micromolecule polypeptide carrier is bonded with functional mimetic peptides including cell adhesion molecule mimetic peptides, cytokine mimetic peptides and chemotactic factor mimetic peptides through covalent bonds to form a self-assembled nanostructure, and the nanostructure hydrogel stent with an immune cell collection function is formed through self-assembling in hydrogel by adjustment of the concentration of the hydrogel. The stent can be used for tumor treatment, injury repair and the like. The functional polypeptide is protected in the hydrogel stent in a three-dimensional existence form, so that in-vivo survival time is prolonged, and the functional polypeptide is in full contact with the immune cells so as to be suitable for recruitment and proliferation of the immune cells, a resistance and repairing effect for diseases, such as tumors, wounds and the like, which perform the function on the basis of the immune cells can be improved, and finally, tumor immunotherapy and wound repair effects are improved.

The invention relates to a spermatogonial cell culture medium and a culture method of hexagrammos otakii, and belongs to the field of cell biology, the spermatogonial cell culture medium comprises the following components: a mixed culture medium, MEM non-essential amino acid with the volume ratio of 1%, double antibodies with the volume ratio of 1%, fish serum with the volume ratio of 1%, growth factors, nutrient substances and fetal calf serum with the volume ratio of 5%. The mixed culture medium is formed by mixing L-15 and DMEM / F12 according to the mass ratio of 1: 1. The culture medium and the culture method are used for culturing the Hexagrammos otakii spermatogonium which is separated and purified in vitro, the spermatogonium which is relatively high in purity and has dryness and proliferative activity can be obtained, and a basic experimental material is provided for research on a regulation mechanism of marine fish spermatogonium transplantation and spermatogonium proliferation and differentiation.

The invention discloses lactobacillus plantarum-enriched low-temperature long-time fermented milk and a preparation method thereof, and belongs to the technical field microbiology and the technical field of fermentation. According to the method, lactobacillus plantarum is inoculated to raw material milk which is added with fruit and vegetable juice to obtain fermented milk; reproduction and fermentation of lactobacillus plantarum in a cow milk fermentation system can be successfully realized; the fermented milk prepared by utilizing the method is fermented for 24-48 hours at 30-37 DEG C, the number of living lactobacillus plantarum in the fermented milk can be 1.2*10<9>CFU / g, and the problem of poor flavor (beany or bitter flavor) of the product caused when yeast powder, soybean protein, soy peptone or hydrolyzed soybean protein is used for promoting fermentation of lactobacillus plantarum in cow milk can be avoided, and large-scale application of lactobacillus plantarum in fermented milk products can be greatly improved.