Cinnamomum camphora somatic embryogenesis and tissue culture method

Abstract

The invention discloses a cinnamomum camphora somatic embryogenesis and tissue culture method, and belongs to the technical field of plant tissue culture. The method comprises the following steps: collecting immature fruits as explants, sterilizing, cutting the fruits, and taking out immature zygotic embryos; carrying out induction culture on the somatic embryos and the embryogenic calluses, carrying out proliferation, maturation and germination on the somatic embryos and the embryogenic calluses, and carrying out breeding culture and rooting culture on somatic embryo seedlings to obtain seedlings; realizing the proliferation, maturation and germination of the somatic embryos and the embryogenic calluses by the transformation of the cinnamomum camphora somatic embryos and the embryogenic calluses in the same stage through a general culture medium for proliferation, maturation and germination of somatic embryos to generate somatic embryo seedlings. According to the method, the general culture medium for proliferation, maturation and germination of somatic embryos can realize conversion of camphor tree embryogenic calluses into somatic embryo seedlings within 3-4 months, a large number of rooting camphor tree somatic embryo seedlings can be obtained within about 6 months, and the method has the advantages of omitting operation procedures, being high in efficiency and being simple and convenient to use.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for somatic embryogenesis and tissue culture of camphor tree. Background technique [0002] Cinnamomum camphora (Cinnamomum camphora) belongs to the genus Lauraceae (Lauraceae), has another name called camphor, an evergreen tall tree, and is a second-class national key protected wild plant in my country. Camphor tree is a precious biological resource of broad-leaved trees, which plays a very important role in the global economy and ecosystem. Camphor tree is an important component tree species in my country's subtropical evergreen broad-leaved forest, and it is also an important timber and special economic tree species in my country. Camphor wood is one of the four famous woods in my country. Its wood material is excellent, its color is bright, its texture is staggered, it has a special aroma, it is insect-resistant, water-resistant, and has a l...

AI Technical Summary

Problems solved by technology

In the existing camphor tree somatic embryogenesis technology, the existence process of embryogenic callus proliferation, induction of mature somatic embryos, and germination of adult somatic embryo seedlings is complicated, the required time is long, and the technology is still immature, and the embryogenic callus and somatic embryos are easy to Browning, many deformed embryos, low seedling transfer rate, etc.

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