Pharmaceutical composition for in vitro amplification of cytotoxic gamma delta T-lymphocytes and amplification method of pharmaceutical composition
An in vitro amplification and cell technology, applied in the field of immunology, can solve problems such as complex methods, and achieve the effect of simple procedure, low cost and high clinical applicability
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Embodiment 1
[0078] Take the peripheral blood of the patient whose number (#) is 1, and amplify according to the amplification method provided by the present invention. The result is as figure 1 shown. The data on the proportion of cytotoxic γδ T cells are entered in Table 1.
[0079] Among them, the concentration of ZOL is 1umol / L, the activity of IL-2 is 100U / mL, and the concentration of LEN is 1umol / L.
[0080] Control is a blank control, that is, no drugs are added, only cell culture fluid and cells are added. ZOL is a single-drug control, IL-2 is a single-drug control, LEN is a single-drug control, and the rest are drug combinations.
[0081] In the figure, the value in the upper right quadrant of each figure represents the proportion of cytotoxic γδT cells. The larger the value in the upper right quadrant, the higher the proportion of cytotoxic γδT cells in the culture system and the greater the number of cells.
[0082] According to the detection principle of flow cytometry, the...
Embodiment 2
[0084] Take the peripheral blood of the patient whose number (#) is 8, and amplify according to the amplification method provided by the present invention. The result is as figure 2 shown. The data on the proportion of cytotoxic γδ T cells are entered in Table 1.
Embodiment 3
[0086] Take the peripheral blood of the patient whose number (#) is 9, and amplify according to the amplification method provided by the present invention. The result is as image 3 shown. The data on the proportion of cytotoxic γδ T cells are entered in Table 1.
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