32results about How to "High scale" patented technology

The invention provides a formate dehydrogenase having high specific activity, small Km values for formate and NAD, broad temperature and pH ranges of stability, broad temperature and pH ranges for action, and capable of regenerating a coenzyme with good efficiency in case of being present in an enzymatic reduction reaction system without inactivated.The invention provides the enzyme mentioned above which has characteristics suited for industrial application by screening for soil formate dehydrogenase-producing microorganisms. The invention further provides a DNA containing the gene coding for the enzyme of the invention, a recombinant DNA constructed using a vector, and a transformant obtained by using this plasmid. The invention further provides a process for producing the formate dehydrogenase of the invention by using any microorganism of the genus Thiobacillus or a transformant constructed by using a formate dehydrogenase gene derived from above strain of microorganism, and a process for regenerating a coenzyme by using said enzyme in an enzymatic reduction system.

A 4-phenyl-4-oxo-2-butenoate derivative is stably supplied in a short period of time, at low cost, in high purity and on an industrial scale by a process for producing the 4-phenyl-4-oxo-2-butenoate derivative, which comprises simultaneously or continuously reacting a sulfuric ester, an aromatic hydrocarbon and a maleic anhydride derivative.

Synthesis of biphosphonate compounds can be advantageously carried out in a solvent / diluent comprising a compound of formula (3)wherein R is hydrogen or a C1-C6 alkyl group; each of R1 is a C1-C6 alkyl group or both R1 groups are linked to form a C1-C3 alkylene group; and R2 is hydrogen, a C1-C6 alkyl group, a C1-C6 alkoxy group, or is linked together with R to form a C3-C7 carbon ring.

The invention discloses a forest disease and pest monitoring and early warning method based on unmanned plane image analysis. The method includes the steps of: S1: shooting a forest area image through a camera carried on an unmanned plane; S2: performing early-stage preprocessing on an image collected by an image collection unit, so as to improve image data and enhance image features; S3: performing image segmentation on the forest area image through an improved fuzzy C-mean algorithm to determine the position of a forest area in the image; S4: performing segmentation on the forest area image through a mark watershed algorithm based on a mixed template to determine the position of a pest plague area are in the image; and S5: on the basis of determining the position of the pest plague area, and in combination with ground survey data, grading the pest plague degree of the pest plague area. The forest disease and pest monitoring and early warning method uses the unmanned plane to carry the camera to shoot data such as pictures of the specified forest area, realizes functions of positioning of the disease and pest area of the forest area, disease and pest grading and early warning and the like, and can satisfy requirements of timely, comprehensive and efficient monitoring and early warning of a disease and pest situation of the forest area.