Culture vessel for cell culture and cell sorting

Abstract

The invention belongs to the technical field of medical treatment and laboratory equipment, and relates to a novel cell culture vessel related to cell culture. The cell culture vessel mainly consists of a vessel frame, protecting films, end covers, press frames and other components, wherein a plurality of ports can be arranged on the two side surfaces of the vessel frame, the protecting films are pressed on the upper and lower two end surfaces of the vessel frame through the press frames, and then the end covers are screwed on the ports of the vessel frame to form a closed cavity in the whole frame body; also, the ports can be connected with external instruments and equipment to realize continuous perfusion of a culture solution and cell gathering during cell culture. The cell culture vessel is characterized in that cell proliferation and cell differentiation can be realized without the need of filling gaseous substances such as O2, CO2 and N2 required by cell culture, and cell sorting and culture can be carried out in the same culture vessel. The cell culture vessel has a simple structure and convenient use, and the inner space of the culture vessel can realize proliferation and co-culture of various types of cells.

Description

technical field [0001] The invention belongs to the technical field of medical treatment and laboratory equipment, and relates to a novel cell culture container related to cell culture. Background technique [0002] With the rapid development of modern biopharmaceutical technology, large-scale cultivation of animal cells is used to produce biological products such as antibody drugs, genetic engineering drugs, and human and veterinary vaccines. Especially in recent years, in fields such as the production of pharmaceuticals, gene therapy, regenerative medicine, and immune cell therapy, it is required to efficiently culture cells, tissues, and microorganisms in large quantities in an artificial environment. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. One of the core technologies for...

AI Technical Summary

Problems solved by technology

The stirred cell culture tank adopts the mechanical stirring method inserted into the tank to promote the flow of the liquid. The structure of the stirring device and its lubricating substances often cause the problem of microbial contamination during work. Bacterial contamination leads to cell death

In order to increase the dissolved oxygen level, it is usually achieved by blowing gas under the liquid surface, but whether it is mixed gas or pure oxygen, it has its negative effects. Because the bubbles burst on the liquid surface, their explosive force can damage animal cells. Too much pure oxygen can also cause cell poisoning. In addition, when cultivating in medium and small volumes, the reactor cannot be moved due to the stainless steel tank and pipeline of the traditional stirred tank, and the environmental requirements are high, so high-pressure steam must be used for heating and sterilization. , which requires special conditions such as steam source, which greatly limits the versatility and convenience of the reactor

[0007] The various cell culture containers widely used in the society listed above have some defects and limitations in structure and performance, and cannot achieve high-efficiency, large-scale, and diverse cultivation of various types of cells, thereby affecting cell biology. Broad adoption of technology at scale in society

Images