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A twig tissue culture propagation method for cinnamomum camphora (L.) presl

A technology of sassafras oil and camphor, which is applied in the field of plant propagation, can solve the problems of low bud proliferation coefficient, slow growth, vitrification, etc., and achieve the effects of saving seedling materials, growing rapidly, and accelerating production

Active Publication Date: 2016-08-10
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of sassafras camphora with good growth effect and rapid multiplication for the problems of browning or vitrification of the primary buds in the tissue culture process of sassafras oil type camphor tree, serious death, low bud proliferation coefficient and slow growth. Tissue culture propagation method of olein type camphor tree twigs

Method used

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  • A twig tissue culture propagation method for cinnamomum camphora (L.) presl
  • A twig tissue culture propagation method for cinnamomum camphora (L.) presl
  • A twig tissue culture propagation method for cinnamomum camphora (L.) presl

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Effect test

Embodiment 1

[0029] In forest stands, green belts or scattered camphor trees, choose a single plant with a straight and round trunk, lush branches and leaves, and fast growth. Smell the leaves of the trees with your nose. According to the quantitative and qualitative analysis of safrole, the yield of leaf leaf oil is more than 1.5%, and the plants with leaf oil safrole content of more than 90% are determined to be sasrole-type camphor trees. In the sunny climate for 3 consecutive days in April 2014, the year-old shoots that grew robustly outside the canopy of sassafras camphor trees were collected as explants. Rinse the explants under tap water for 10 min, then remove the leaves of the explants, then soak the explants in a solution of emamectin benzoate with a volume concentration of 0.1%, and control the soaking time for 15 min. Finally, wash the medicine with pure water, cut the explants into stem segments with at least one axillary bud and a length of 1.5-2.0 cm, and place them in conta...

Embodiment 2

[0036] In forest stands, green belts or scattered camphor trees, choose a single plant with a straight and round trunk, lush branches and leaves, and fast growth. Smell the leaves of the trees with your nose. According to the quantitative and qualitative analysis of safrole, the yield of leaf leaf oil is more than 1.5%, and the plants with leaf oil safrole content of more than 90% are determined to be sasrole-type camphor trees. In the sunny climate for 4 consecutive days in May 2014, the year-old shoots that grew robustly outside the canopy of sassafras camphor trees were collected as explants. Rinse the explants under tap water for 12 minutes, then remove the leaves of the explants, then soak the explants in emamectin benzoate solution with a volume concentration of 0.1%, and control the soaking time for 150 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least one axillary bud and a length of 1.5-2.5 cm, and place them in con...

Embodiment 3

[0043]In forest stands, green belts or scattered camphor trees, choose a single plant with a straight and round trunk, lush branches and leaves, and fast growth. Smell the leaves of the trees with your nose. According to the quantitative and qualitative analysis of safrole, the yield of leaf leaf oil is more than 1.5%, and the plants with leaf oil safrole content of more than 90% are determined to be sasrole-type camphor trees. In the sunny climate for 3 consecutive days in June 2014, the year-old shoots that grew robustly outside the canopy of sassafras camphor trees were collected as explants. Rinse the explants under tap water for 13 minutes, then remove the leaves of the explants, then soak the explants in a solution of emamectin benzoate with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least one axillary bud and a length of 2.5-3.0 cm, and place them in...

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Abstract

A twig tissue culture propagation method for cinnamomum camphora (L.) presl is disclosed. The method is characterized in that the method includes explant selecting, explant processing, primary shoot induction culture, multiplication culture, strong-shoot culture and rooting culture, annual twigs robust in growth and at the periphery of the crown of the cinnamomum camphora (L.) presl are collected and adopted as explants, the explants after being trimmed, sterilized and disinfected are inoculated into a primary shoot induction culture medium to obtain primary buds, the primary buds are inoculated to a multiplication culture medium and subjected to 4-5 cycles of multiplication culture to form cluster buds, the cluster buds are inoculated to a strong-shoot culture medium and cultured, and a single shoot the height of which is not less than 2 cm is inserted into a rooting culture medium and subjected to root induction. The method is simple and convenient in operation, short in culture period, high in subcultured shoot yield and good in subcultured shoot quality. The multiplication constant is 5 or above. The method meets technique requirements on industrialization of tissue-cultured seedlings, and has good economic benefit, ecological benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to the tissue culture technology of camphor tree, in particular to a method for tissue culture propagation of sassafras-type camphor tree twigs. Background technique [0002] Sassafras camphor tree is a kind of chemical camphor tree (Cinnamomum camphora (L.) Presl), also known as camphor, which is a national second-class protected plant, mainly distributed in Jiangxi, Hunan, Guangdong, Fujian, Hainan, Guangxi, Sichuan, Taiwan and other places, Vietnam, South Korea and Japan are also distributed. Grows in hills and low mountains. It is a multi-purpose excellent tree species that is used for wood collection, oil, flavor and fragrance, medicine, and landscape gardening. Sasrole has the unique aroma of trees and is a very useful natural fragrance. ) and other products, it is also an important pharmaceutical intermediate, which is widely used in industrial production such as l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 安家成蔡玲黄金使李开祥梁晓静丘米杨素华黄宏喜
Owner GUANGXI FORESTRY RES INST
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