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Tissue culture and rapid propagation method suitable for tung trees

A technology of tissue culture rapid propagation and tung tree, which is applied in the field of plant propagation, can solve the problems of slow growth and low bud multiplication coefficient, and achieve the effects of large yield of subculture buds, simple operation process and saving seedling raising materials

Inactive Publication Date: 2017-05-31
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for tissue culture and propagation of tung tree twigs with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the tissue culture process of tung tree

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the tung tree as explants;

[0026] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0027] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 4.0 mg / L+NAA 2...

Embodiment 2

[0033] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the tung tree as explants;

[0034] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 5.0 ...

Embodiment 3

[0041] (1) Selection of explants: Collect semi-lignified, vigorously growing twigs of the tung tree as explants;

[0042] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified MS medium+6-BA 6.0 ...

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Abstract

The invention discloses a tissue culture and rapid propagation method suitable for tung trees. The method comprises the following processes: selecting explants, treating the explants, carrying out primary bud induction culture, carrying out propagation culture, culturing strong buds and carrying out rooting culture; the method comprises the concrete steps of collecting semi-lignified tung tree twigs, which grow robustly in the very year, as the explants, carrying out pruning and sterilizing treatment on the explants, and then inoculating an initial bud induction medium with the explants so as to obtain initial buds; inoculating a propagation medium with the initial buds, and carrying out the propagation culture for 3-4 cycles to form multiple shoots; inoculating a strong bud culture medium with the multiple shoots for culturing; finally, inserting a single bud with the height of more than or equal to 2cm into a rooting medium for inducing a root. After the tissue culture and rapid propagation method is used, the seedling raising period of the tung trees is shortened, and seedling raising materials are saved; the method solves the problems that the traditional seedling raising method needs more seedling raising materials, is long in cycle, and the like, greatly lowers the production cost, increases the production efficiency, and has better economic benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to a rapid propagation method suitable for tissue culture of tung tree. Background technique [0002] Tung tree (Vernicia fordii (Hemsl.) Airy Shaw), Euphorbiaceae Tung genus, is a deciduous tree with gray bark, thick branches, oval leaves, monoecious flowers, calyx densely covered with brown puberulent ; petals white, with light red veins, obovate, ovary densely pilose, drupe nearly globose, seed coat woody. The flowering and fruiting period is from March to September. Distributed in Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Hubei, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan and other provinces and regions in China. Tung, together with camellia oleifera, walnut, and black tallow, are called the four major woody oil plants in China. Tung tree is born in the area above 1000 meters, likes warmth and avoids severe cold. A temperature ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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