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Tissue engineering 3D stent based on bean curd as well as preparation method and application thereof

A technology of tissue engineering and tofu, applied in biochemical equipment and methods, tissue regeneration, 3D culture, etc., can solve the problems of difficult control of chitosan degradation process, poor mechanical properties of gelatin, poor mechanical properties, etc., to achieve benefits For cell adhesion and growth, stable and reliable comprehensive performance, easy to obtain effects

Inactive Publication Date: 2018-06-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in these scaffolds, such as the degradation process of chitosan is not easy to control, and the mechanical properties are not good; gelatin has disadvantages such as poor mechanical properties, brittle quality, and poor water resistance; rather unstable

Method used

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  • Tissue engineering 3D stent based on bean curd as well as preparation method and application thereof
  • Tissue engineering 3D stent based on bean curd as well as preparation method and application thereof
  • Tissue engineering 3D stent based on bean curd as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Preparation and cell culture of a tofu-based tissue engineering 3D scaffold

[0064] 1. Preparation method:

[0065] (1) Preparation of tofu: raw materials such as soybeans and water are prepared into tofu by traditional techniques;

[0066] (2) Synthesis of 3D scaffolds for tissue engineering: Cut the obtained tofu into small cubes with a length, width, and height of 2 cm, pre-freeze at -20°C for 12 hours, and then vacuum freeze-dry at -60°C to -50°C for 48 hours ( The vacuum degree is controlled to be 10Pa), and then the tofu-based tissue engineering 3D scaffold can be obtained.

[0067] 2. Cell culture

[0068] (1) The prepared tofu-based tissue engineering 3D scaffold was washed 2 to 3 times with sterile PBS solution to remove the dissolved matter, and then frozen at -80°C, then transferred to a freeze dryer for 48 hours, and then epoxy Ethane gas sterilization for about 24 hours;

[0069] (2) Transfer the thin-layer circular slices to a 6-well plate, ...

Embodiment 2

[0076] Example 2 Preparation and cell culture of a tofu-based tissue engineering 3D scaffold

[0077] 1. Preparation method:

[0078] (1) Preparation of tofu: Soak the soybeans in deionized water to swell for 6 hours, drain the water, and homogenize at room temperature to obtain soybean milk; add 2 times the volume of deionized water at 90°C to the soybean milk for dilution to obtain the original pulp Dilution: filter the original pulp dilution while hot, add bittern salt (the addition of bittern salt accounts for 2.5%~4% of soybean consumption) in the filtrate, after separating out the bean curd flower precipitation, remove unnecessary moisture, press while hot, That is tofu.

[0079] (2) Synthetic tissue engineering 3D scaffold: same as Example 1.

[0080] 2. The cell culture method is the same as in Example 1.

[0081] 3. Product performance and characteristics

[0082] (1) The tofu-based tissue engineering 3D scaffold prepared in this embodiment is as follows: figure ...

Embodiment 3

[0087] Example 3 Preparation and cell culture of a tofu-based tissue engineering 3D scaffold

[0088] 1. Preparation method:

[0089] Other conditions are with embodiment 1, and difference is:

[0090] After the obtained tofu is cut, it is pre-frozen at 0° C. for 24 hours, and then vacuum freeze-dried at -80° C. to -70° C. for 36 hours.

[0091] 2. The cell culture method is the same as in Example 1.

[0092] 3. Product performance and characteristics

[0093] The scaffold maintains the original natural structure of tofu in appearance and is properly loose; its surface is porous network-like, with pores of different sizes distributed, the internal structure is loose, and interconnected channels are many and obvious; in the scaffold There are a large number of cells attached in the medium, and the cell shape is good; and the biological safety is good, the water resistance is good, and the product properties are stable and reliable.

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Abstract

The invention discloses a tissue engineering 3D stent based on bean curd as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly pre-freezing the bean curd, and then carrying out freeze-drying treatment to obtain the tissue engineering 3D stent based on bean curd. A loose and porous structure is formed in the tissue engineering 3D stent prepared by taking the bean curd as the raw material; various and obvious pore channels are communicated with each other; the requirement of the cell growth on the stent material structure is met; after the prepared 3D stent is simply treated, the 3D stent can be directly applied to the tissue engineering field; the cells achieve effective adhesion, growth and proliferation on the surface of the3D stent; the 3D stent has an obvious promotion effect on the growth of the cells; meanwhile, the product is stable in structure, high in biocompatibility and excellent in mechanical property; in addition, the tissue engineering 3D stent is simple to operate and high in repeatability; the condition is easy to implement; the prepared product is reliable in performance and has broad application prospects in the tissue engineering field.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering. More specifically, it relates to a tofu-based tissue engineering 3D scaffold and its preparation method and application. Background technique [0002] The abnormality of body function caused by damage or loss of tissues or organs is an important reason affecting human health. At present, the amount of organ donations that can meet clinical needs is far from enough. Although autologous transplantation and allogeneic transplantation have achieved certain clinical results, they have long-term adverse reactions to patients, and their clinical application is very limited. With the needs of medical development, tissue engineering came into being. It applies the principles of life science and engineering to develop functional substitutes for damaged tissues so as to restore body functions. The main content of its research is to prepare biological scaffolds that can mimic human extracellular...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/56C12N5/07
CPCA61L27/3637A61L27/50A61L27/56A61L2430/34C12N5/0062C12N5/06C12N2513/00C12N2533/90
Inventor 吴钧黄骏欧阳宏顾志鹏
Owner SUN YAT SEN UNIV
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