Proliferation and rapid propagation method of green spheroids of ferns submerged intermittently
A technology for green spheroids and ferns, which is applied in the field of proliferation and regeneration of fern green spheroids, can solve the problem that the overall cleaning and sterilization of the aseptic manual inoculation operation device is not easy, and it is not easy to disassemble or move and control components. complex problems, to achieve the effect of high utilization rate of culture space, simple and compact structure, and saving culture space
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[0036] Embodiment 1 Pteridophyta GGB proliferates and propagates rapidly and cultivates
[0037] 1. Take fern leaves and rhizomes of mature sporophytes, wash them with running water for 2 hours, soak them in 75% alcohol for 10-20 seconds, sterilize them with HgCl2 for 6 minutes, and wash them with sterile water 4 times to obtain sterile GGB induction materials.
[0038] 2. Put leaves, rhizomes and other materials into the induction medium, about 3-4 weeks, GGB appears on the explants.
[0039] 3. First stack a plurality of empty sieve trays in the fixed frame, then put the empty object component into the main body of the culture container, and then engage the screw thread to seal the cover and the main body of the culture. In addition, the outer opening of the main body of the culture container is sealed with cotton balls, and the whole device is to be sterilized under high temperature and high pressure;
[0040] 4. The silicone hose can be sealed with newspaper and sterilize...
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