Method of tissue culture and propagation expansion of iris

A technology of iris group and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of high rooting cost, slow iris reproduction rate, etc., to improve survival rate, reduce tissue culture cost, and reduce cost Effect

Inactive Publication Date: 2019-05-28
DINGZHOU LYUGU AGRI TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for tissue culture and multiplication of iris, which mainly solves the technical problems of slow propagation rate of iris and high cost of rooting which restrict the rapid industrial seedling cultivation of iris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the method for a kind of Iris (India leader) tissue culture expansion of this example, its steps are:

[0019] a. selection and disinfection of explants: select healthy plants without diseases and insect pests, cut shoots (the white part of the root section, the volume of the cut part is larger than the shoots), clean the surface with a hairbrush, rinse under running water, Disinfect with alcohol on the ultra-clean workbench, then rinse with sterile water 2-3 times, transfer to mercuric chloride solution for disinfection, and then rinse with sterile water 3-4 times, and then use it for later use.

[0020] b. Induce sprouts to germinate: In the ultra-clean workbench, cut off the excess part of the sprouts obtained in the previous step, and inoculate them into the medium for inducing sprouts. The medium components are: 1 / 2MS basic medium + 6-BA

[0021] 3-4mg / L+IBA0.2-0.4mg / L+white sugar 25-35mg / L+agar 5g / L, adjust the pH to 5.8;

[0022] c. Proliferation c...

Embodiment 2

[0026] Embodiment 2: the method for a kind of iris (sky blue) tissue culture expansion of this example, its steps are:

[0027] a. selection and disinfection of explants: select healthy plants without diseases and insect pests, cut shoots (the white part of the root section, the volume of the cut part is larger than the shoots), clean the surface with a hairbrush, rinse under running water, Disinfect with alcohol on the ultra-clean workbench, then rinse with sterile water 2-3 times, transfer to mercuric chloride solution for disinfection, and then rinse with sterile water 3-4 times, and then use it for later use.

[0028] b. Induce sprouts to germinate: In the ultra-clean workbench, cut off the excess part of the sprouts obtained in the previous step, and inoculate them into the medium for inducing sprouts. The medium components are: 1 / 2MS basic medium + 6-BA

[0029] 3-4mg / L+IBA0.2-0.4mg / L+white sugar 25-35mg / L+agar 5g / L, adjust the pH to 5.8;

[0030] c. Proliferation cultu...

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PUM

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Abstract

The invention relates to the technical filed of industrialized seedling production, in particular to a method for tissue culture and propagation expansion of iris. The technical problems of slow propagation expansion, long rooting period, complicated rooting procedure, high production cost and the like which restrict rapid industrialized seedling production of the iris are solved. The method comprises the steps that first an explant is selected and disinfected, then tender shoots are induced to germinate, then proliferation culture and rooting culture are performed, and finally domestication and transplanting are performed. The medium which induces tender shoot germination is prepared form the components: modified MS basic medium, 1-2mg / L of 6-BA, 0.3-0.5mg / L of IBA, 25-35mg / L of sugar and5g / L of agar, pH is adjusted to 5.8, and the modified WPM basic culture replaces original potassium nitrate and calcium chloride with calcium nitrate hydrate and potassium sulfate; and the root dripping treatment is carried out again by domestication and transplanting.

Description

technical field [0001] The invention relates to the technical field of industrialized seedling cultivation, in particular to a method for tissue culture propagation of iris and rooting outside a bottle. Background technique [0002] Iris, also known as: blue butterfly, purple butterfly, flat bamboo flower, etc., belongs to the order Asparagus, and is a perennial herb of the family Iridaceae. With the improvement of people's aesthetics, ordinary greening can no longer meet people's needs. People are eager for novelty, difference, and eye-catching garden landscapes. Abundant plant materials are the elements of landscape formation, and the three-dimensional configuration of trees, shrubs and grasses is the principle of landscape configuration and the requirement of biodiversity in an ecosystem. As ground cover plant material, besides the lawn, it is a perennial flower with various types and colors, strong stress resistance, extensive cultivation and management, and the charact...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈小奎杨小兵庞曼杨宝英张永伟李芳陈欣
Owner DINGZHOU LYUGU AGRI TECH DEV CO LTD
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