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Tissue culture method of Stachys siebolidii Miq

A technology for tissue culture and Cordyceps ginseng, which is applied in the field of plant tissue culture to achieve the effects of not being restricted by seasons, shortening the reproductive cycle, and having consistent growth states.

Active Publication Date: 2019-04-05
XUZHOU VOCATIONAL COLLEGE OF BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on tissue culture of Cordyceps ginseng of Labiatae

Method used

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  • Tissue culture method of Stachys siebolidii Miq

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Extraction and processing of explants: Using the high-quality Cordyceps ginseng seedlings successfully introduced in the school base as materials, on April 12, 2018, the young stems from the vigorously growing, healthy and pest-free plants were cut After trimming and removing the leaves, take them back to the laboratory, wash them with washing powder water for 10 minutes to remove surface dirt, rinse them with running water for 30 minutes, and transfer them to the ultra-clean workbench for later use.

[0026] (2) Surface disinfection and induction of explants: the cleaned young stems were treated with 75% ethanol for 10 s, sterilized with 0.1% mercuric chloride for 6 min, rinsed with sterile water for 5-7 times, and the stems were Cut into 1-2 cm single-bud stem segments and inoculate them in induction medium. The medium composition is MS+6-BA1.0 mg / L+NAA0.1 mg / L, sucrose 30g / L, agar 7g / L, pH 5.8. The number of inoculations is 30 bottles, with 1 explant in each bot...

Embodiment 2

[0032] (1) Extraction and treatment of explants: On July 4, 2018, the young stems were cut off from the vigorously growing, healthy and pest-free Cordyceps ginseng plants introduced in the school base, and the pretreatment method was the same as in Example 1.

[0033] (2) Surface disinfection and induction of explants: the cleaned young stems were treated with 75% ethanol for 10 s, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water for 5-7 times, and the stems were Cut into 1-2 cm single-bud stem segments and inoculate them in induction medium. The composition of the medium was MS+6-BA1.0 mg / L+NAA0.1 mg / L+activated carbon 1.5 g / L, sucrose 30g / L, agar 7g / L, pH 5.8. The number of inoculations and culture conditions are the same as in Example 1, and will not be repeated.

[0034] (3) Adventitious bud differentiation and proliferation: On July 12, a large number of lateral buds sprouted and the average induction rate was 86.37%. When the new shoots grow ...

Embodiment 3

[0039] (1) Extraction and treatment of explants: On September 1, 2018, the young and tender stems of Cordyceps sinensis were cut off at the school base to make explants, and the pretreatment method was the same as in Example 1.

[0040] (2) Surface disinfection and induction of explants: the cleaned young stems were treated with 75% ethanol for 20 s, sterilized with 0.1% mercuric chloride for 4 min, rinsed with sterile water for 5-7 times, and the stems were Cut into 1-2 cm single-bud stem segments and inoculate them in induction medium. The components of the induction medium, the number of inoculations and the culture conditions are the same as those in Example 2, and will not be repeated here.

[0041] (3) Adventitious bud differentiation and proliferation: After one week of induction, side buds sprouted, and the average induction rate was 88.54%. The new adventitious buds were cut off to continue the multiplication culture. The composition of the culture medium is MS+6-BA...

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Abstract

The invention provides a tissue culture method of Stachys siebolidii Miq. According to the method, a propagation system of commercial test-tube seedlings is obtained through the steps of induction, propagation expanding, seedling strengthening, rooting and the like of Stachys siebolidii Miq immature stem segments in an in-vitro condition, thereby shortening the propagation cycle, increasing the proliferation rate, making the seedlings consistent in grow state, excellent in quality and not restricted by seasons, achieving anniversary production, and achieving the propagation of high-quality commercial Stachys siebolidii Miq seedlings through a tissue culture mode.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of Cordyceps ginseng. Background technique [0002] Cordyceps ginseng, also known as ground ginseng, is a perennial herb of the Lamiaceae. The plant height is 50-70cm, and the underground stolon is 5-6cm long. It looks like a caterpillar fungus and is as thick as a finger. It is white, crisp and tender, rich in nutrition and full of treasures. It can not only be eaten as vegetables, but also used as medicine after drying. Its health care and medicinal value It is equivalent to Cordyceps sinensis and has good medicinal and edible functions and market benefits. [0003] In recent years, with people's crazy demand for environmental natural resources, leading to the deterioration of the ecological environment and the extensive use of pesticides, the traditional Cordyceps ginseng is almost facing extinction. The industrial production of Cordy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陶佩琳汪希强张旭东
Owner XUZHOU VOCATIONAL COLLEGE OF BIOENG
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