64results about How to "Achieve annual production" patented technology

The present invention relates to plant cultivation technology, and is greenhouse asparagus cultivating process. The green house asparagus cultivating process includes the steps of transplanting asparagus seedling in greenhouse, picking up spring asparagus in January to March, leaving spring stubble in April, picking up summer asparagus in May to August, leaving autumn stubble in September, picking up autumn asparagus in October, applying base fertilizer for three times before picking up, applying organic fertilizer and top dressing properly, sterilizing soil, and preventing and controlling diseases and pests. The present invention has high asparagus yield, less diseases and pests, less pollution to asparagus and high asparagus quality.

The invention discloses an industrial needle mushroom cultivation method. The industrial needle mushroom cultivation method includes preparing a cultivation medium, switching into a solid cultivation medium for cultivation and harvesting. The industrial needle mushroom cultivation method has the advantages that cultivation medium materials are easily available and are recycled from multiple kinds of plants and agricultural product waste, and accordingly, the method has the industrial characteristic of making waste profitable, natural matter and energy cycle can be accelerated, and recycling of animal-plant agricultural by-products is benefited more effectively; the method is beneficial to development of ecological agriculture, high-efficiency agriculture and recycling agriculture in China and conforms to the megatrend of China's agricultural industry development and construction of new socialist countryside.

The invention belongs to edible fungi production technology field, and discloses a method to cultivate agrocybe chaxingu in a year by using civil air defense facilities. The method is characterized in that the cool in summer and warm in winter environmental feature of civil air defense facilities is taken advantage of to provide a suitable growing condition, so that the annual cultivation of agrocybe chaxingu is realized without air-conditioning equipment. The invention can greatly save energy, effectively reduce operation cost and enhance economic returns.

The invention discloses a leaf cutting propagating method of a primulina hance plant. The leaf cutting propagating method of the primulina hance plant includes the following steps of (1) selecting cutting time, (2) preparing cutting slips, (3) preparing cutting bed, (4) cutting, (5) managing after cutting, (6) transplanting the primulina hance plant into pots. By means of the leaf cutting propagating method of the primulina hance plant, rapid propagation of the primulina hance plant is achieved, seedlings are rounding and normal in plant type, little difference between the seedlings and stock plants exists, and the quality of seedlings is good and identical. In addition, by means of the leaf cutting propagating method of the primulina hance plant, annual production of the primulina hance plant can be achieved, possibility is provided for commercial production of the primulina hance plant and the cost of the commercial production of the primulina hance plant is reduced.

An annual cultivation method for coprinus comatus sequentially comprises the following production methods: the technological processes of culture production, fermentation treatment of culture medium, sterilizing inoculation, hairy fungus culture, soil sealing, mushroom production, and the like, are carried out; the culture is produced by a liquid seed production method; reed residues and needle mushroom leftovers are used as culture medium for fermentation treatment; cultural bags adopts open type inoculation, and a two-stage type cultural method is used; the inoculation and the hairy fungus culture are carried out in ordinary steel tube greenhouse; the soil sealing and mushroom production are carried out in the environment of civil air-defense construction. The invention utilizes idle civil air-defense facilities, does not need refrigeration plants without energy consumption and can always keep the environmental temperature of mushroom production at 18-22 DEG C, thereby being capable of not only ensuring coprinus comatus to grow normally but also controlling the generation of important disease palmatum bacteria of coprinus comatus so as to efficiently realize annual production of coprinus comatus; in addition, the mushroom production time can be freely controlled by controlling the soil sealing time so that the biological conversion rate is more than 80% and the yield can be improved more than 25% compared with ordinary cultivation.

The invention belongs to the edible fungi production technical field and discloses a method for annually producing drumstick by utilizing air raid shelter. The method is characterized in that: the mode of 'spawn running outside and fruiting in shelter' is adopted, massive drumstick bags are produced in high tunnel and vacant room under good aeration condition outside the shelter in season with appropriate temperature; earthing and fruiting are carried out by utilizing the specific natural environment warm in winter and cool in summer in the air raid shelter in winter and summer when fruiting is difficult to implement outside the shelter, and annual production of drumstick is realized without cooling and heating equipment. The invention greatly saves energy source, effectively reduces production cost and also can freely control earthing time according to market demand in different seasons, fruiting period can be artificially regulated, concentration to appear on the market can be avoided, bioconversion is above 85%, and yield can be improved by 30-20% compared with the conventional cultivation.

The invention relates to a novel pure-long-straw cultivation edible fungus technology, which is characterized in that straws such as rice straws and wheat straws are used for cultivating the edible fungus without being cut (pulverized); the straws are fermented by utilizing effective microorganism beneficial bacterial strain, so that the substrate base stock is high-efficiently softened, and the decomposition and utilization by the hypha can be favored; nutritional soil is used for being made into a heat insulation layer, so not only is the yearly production realized, but also clean water and nutritive liquid stored in the heat insulation layer can be slowly conveyed to bacteria sticks during the later management, and the yield can be guaranteed; large-sized plastic bags are used as bacteria stick bags, so that the cultivation efficiency is multiplied in production compared with that of an ordinary plastic bag; and liquid strain is used for inoculation, and the liquid strain is flow, so that the liquid strain can be permeated and spread into the straws after the inoculation, once the strain is sprouted, all strain can eat and grow fully, dyeing of infectious microbe can be avoided, and rapidness for the growth of mushrooms can be realized.

The invention relates to a method for producing a high-macroelement liquid organic special fertilizer. The method comprises the following steps: selecting raw materials, pretreating the raw materials, performing anaerobic fermentation, performing slag-liquid separation, performing oxygen exposure filtration, mixing and blending and performing refined filtration filling. The content of the macroelement is improved to a high level, and the topdressing problem of organic cultivation is solved; and moreover, the production efficiency is high, the cost is low, and the problems that waste of biogas slurry and sewage is severe even the environment is polluted are solved.

The invention discloses a method for improving winter resistance of turf in Tianjin by adopting municipal garbage compost filtrate. The method mainly comprises the following steps: (1) preparing compost eluent, (2) cultivating lawn plants, and (3) carrying out cold hardening and sampling. The final technical effect shows that cold-hardened turf can fully resist winter in the winter environment ofnot less than 10 DEG C below zero in the outdoor balcony without being provided with a heating facility in the house of Tianjin, and the aim of storing the turf in winter is achieved. Compared with the peripheral turfgrass, the ryegrass seedlings turned green in spring next year has obvious advantages, which means that the garbage compost filtrate plays an important role in lawn plant growing andwinter resisting and plays a guiding role in applying the garbage compost filtrate to the growth of the turf in winter. The technology lays a foundation for the production of the turf all year around.

The invention discloses a stereoscopic frame-type cultivation method of Dictyophora rubrovolvata. The method comprises the four steps: soil preparation and shed erecting; cultivation in baskets; and management in cultivation period and Dictyophora rubrovolvata harvesting treatment. The stereoscopic frame-type cultivation method has the characteristics of short period, high yield, good quality and lower cost.

The invention discloses a diapause induction method of harmonia dimidiate. The diapause induction method comprises the steps of 1) determining the diapause control sensitive stage of the harmonia dimidiate as adjusts just after eclosion and selecting the harmonia dimidiate at the stage as the diapause induction objects, and 2) putting the harmonia dimidiate selected in the step 1) in a diapause induction environment and enabling the harmonia dimidiate to enter the diapause state by regulating and controlling the combination of illumination time each day, temperature and total induction time, wherein the illumination time each day is photoperiod (8-10)L: (16-14) D, the temperature is in the range of 13-15, and the continuous induction time is greater than or equal to 192 hours. The diapause induction method of the harmonia dimidiate is capable of realizing accurate control on the excellent natural predatory enemy insect harmonia dimidiate by controlling the environmental factors in combination necessary treatment measures; the diapause rate can be about 95%; technical guarantee is provided for annual large-scale production of the harmonia dimidiate.

The invention discloses a method for producing vinblastine and / or vincristine. The method comprises: inoculating a periwinkle polyploid cell strain in a synthetic medium, and obtaining a cell rich in vinblastine and vincristine by cultivating the strain for 3 to 7 days at the temperature between 20 and 29DEG C; and the synthetic medium is a liquid culture medium obtained by adding 0 to 5mg / L of plant cell auxin, 0 to 5mg / L of plant cytokinin, 30 to 60g / L of sucrose, 1 to 50mu g / L of acetyl coenzyme A, 10 to 40mu g / L of hydogen peroxide, 0.1 to 2mu mol / L of Benzotriazole methl, 10 to 150mg / L of tryptophan, 10 to 110mg / L of loganin and 10 to 50mg / L of cerium chloride in a minimal medium. The method using periwinkle cells to cultivate and produce vinblastine and vincristine can stably realize the industrialized production of vinblastine and vincristine, and the production cost is rather low, the culture period is short, and the production is insusceptible to the natural environment and weather, so the year-round production can be realized.

The invention discloses a method for improving winter resistance of turf by adopting household garbage compost offscourings. The ryegrass seedlings cultivated by compost and cold-hardened in cold environment with gradually decreased temperature have no obvious difference from the seedlings in the control group in indexes such as protective enzyme, proline and conductivity, but are slightly shorter. The aboveground biomass of the cold-hardened ryegrass seedlings cultivated by compost offscourings is reduced by 26.74% compared with that of the seedlings in the control group and is reduced by 23.21% compared with that of the seedlings with relatively poorer matix nutrition in the filter paper group. The final technical effect shows that the cold-hardened turf can fully resist winter in the winter environment of not less than 10 DEG C below zero in the outdoor balcony without being provided with a heating facility, and the aim of storing the turf in winter and producing all year around is achieved.

The present invention discloses a structure of a novel south and north dual-shed sunlight greenhouse with a middle wall and an operating method. Structurally, the sunlight greenhouse mainly comprises a sunlight greenhouse foundation (1), a south and north shed steel frame prefabricated part connector (2), the middle wall (3), a side wall (4), a cushion working room (5) and a heat exchange control system (6). A sliding door type movable structure of the middle wall (3) plays an important role of regulating the illumination distribution, temperature conditioning and ventilation of a south shed and a north shed. A vertical splitter plate water heat exchange automatic control structure of the heat exchange system (6) is combined with the movable middle wall (3), so that the heat exchange ability of the sunlight greenhouse is improved. The operating method of the sunlight greenhouse mainly comprises the following steps: insulating heat, storing heat, releasing heat and lowering humidity in winter; and ventilating, shading, radiating and cooling in summer, and has the advantages of saving cultivated land, producing all the year, being low in construction cost and the like.

The invention relates to a method for all-year production of coprinus comatus by the utilization of a soil cave. According to the method for all-year production of coprinus comatus by the utilization of the soil cave, grain stillage serves as raw materials for cultivation, the soil cave serves as environment for cultivation, the cultivation mode that spawn running and fruiting are carried out in the soil cave is adopted, and the method includes the eight implementation steps of excavation of the soil cave, production of coprinus comatus seeds, preparation of cultivation materials, fermentation treatment of the cultivation materials, production of coprinus comatus bags, spawn running of the coprinus comatus bags, earth covering and management on fruiting. The manually excavated soil cave is utilized as a production place, the cultivation mode that spawn running and fruiting are carried out in the soil cave is provided, spawn running is directly carried out in the soil cave after the coprinus comatus bags are filled with coprinus comatus, earth covering and fruiting are carried out directly after spawning running is carried out, no cooling or heating equipment is needed, growth of coprinus comatus entities is met in the natural state, and all-year production of coprinus comatus is achieved. The grain stillage is used as the new raw materials of cultivation, the time for processing the cultivation materials is shortened, labor expenses are lowered, and production cost is lowered.

The invention relates to a method for factory sterilized raw material cultivation of organic agaricus brasiliensis, and belongs to the technical field of edible fungi. The method includes the following steps: preparing a medium, filling a ventilating fungus sack with the medium, carrying out high temperature autoclaving, and cooling the medium; inoculating agaricus brasiliensis strains of a cultivated species on the cooled cultivation sack by using an automatic inoculation machine in an aseptic inoculation room, and automatically sealing the cultivation sack by using a sealing machine; carrying out cultivation, laying 2-3 centimeters of soil for covering, letting mycelia climb up the soil layer, accelerating germination, carrying out fruiting management, and finally carrying out harvestingand secondary fruiting management after the harvesting. The yield of the agaricus brasiliensis per square meter is increased from 2-3.5 kg to 5-6 kg by optimizing a medium formula, a cultivation process, cultivation technologies and the like of the agaricus brasiliensis. The production process of the agaricus brasiliensis can completely satisfy the factory production, and can produce organic, safe, pesticide-free, mercury-free and cadmium-free agaricus brasiliensis products at the same time to provide safe and nutritive healthy food for mankind.

The invention belongs to the field of modern organic production of edible fungi, and relates to an industrial cultivation method for organic agrocybe aegerita to overcome defects in existing agrocybe aegerita cultivation methods. The method comprises the steps as follows: firstly, cultivation of a mother strain of agrocybe aegerita; secondly, preparation of a production bag; thirdly, sterilization of the production bag; fourthly, inoculation of the mother strain of agrocybe aegerita into the production bag; fifthly, growth of hyphae and sporophore; sixthly, preventive sterilization of the production bag during growth of sporophore, organic treatment of the recovered production bag after growth period of sporophore, and sterilization of a sporophore growth chamber. According to the industrial cultivation method, a stock is subjected to local independent cultivation, the survival rate of the stock exceeds 99.9%; the raw material formula of the production bag is improved, the efficiency is high, the cost is low, and the yield of agrocybe aegerita is increased; the production environment is standardized, and whole year production is realized; the growth period of hyphae and the growth period of sporophore in the production bag are performed at stages simultaneously, the production efficiency is high, the production period is short, the quality is high, pesticide is not applied, and cultivated agrocybe aegerita reaches the organic standard.

The invention discloses an annual cultivating method for sterilizing cut-flower lily through carbendazim and streptomycin. The method includes the following specific steps of firstly, selecting seed balls, wherein the seed balls pure in variety, free of bacteria and complete in root system are selected; secondly, sterilizing the seed balls, wherein a medium material is selected for use and filtered so that coarse slag can be removed, a disinfectant is prepared to soak the medium material so that the water content of the medium material can range from 40% to 60%, one layer of lily seed balls are placed each time after one layer of wet medium material is scattered, and then the seed balls are packaged after a plastic bag is filled with the seed balls; thirdly, processing the seed balls at the low temperature, wherein the lily seed balls are placed into a refrigeration house at the temperature of 14 DEG C to 16 DEG C to be preprocessed for 8 days to 12 days, the temperature of the refrigeration house is then adjusted to be 2 DEG C to 6 DEG C, and the seed balls are processed at the lower temperature for 30 days to 38 days; fourthly, unfreezing the seed balls to be planted, wherein the seed balls are slowly unfrozen in the environment at the temperature of 8 DEG C to 12 DEG C, the seed balls are planted instantly after being unfrozen, and soil high in water permeability and breathability is laid on the seed balls by 8 cm to 10 cm. By means of the method, cut-flower lily flowers neatly, the lily fresh cut flower quality is improved, the lily flowering phase is adjusted, and the aim of annularly producing lily is achieved.

The invention provides a tissue culture method of Stachys siebolidii Miq. According to the method, a propagation system of commercial test-tube seedlings is obtained through the steps of induction, propagation expanding, seedling strengthening, rooting and the like of Stachys siebolidii Miq immature stem segments in an in-vitro condition, thereby shortening the propagation cycle, increasing the proliferation rate, making the seedlings consistent in grow state, excellent in quality and not restricted by seasons, achieving anniversary production, and achieving the propagation of high-quality commercial Stachys siebolidii Miq seedlings through a tissue culture mode.

A procss for preparing phenylethanoid glycoside from cistanche by bioconversion technique includes adding cane sugar or glucose, indoleacetic acid, and cytokinin to the improved culture medium MS, adding precursor, regulating pH 5.5-7.0 to obtain synthetic culture medium, disinfecting, cooling, inoculating the calli of cistanche to the synthetic culture medium, oscillating culture in dark condition, collecting the cells, freeze drying, extracting in solvent and purifying.

The invention relates to an efficient detoxification method for sweet cherry rootstocks; the method comprises the steps: 1), utilizing sweet cherry rootstock leaf buds in a field as explants for tissue culture, sterilizing and culturing to obtain tissue culture seedlings; 2) subculture; 3) thermal treatment; 4) pre-culturing and loading; 5) vitrification; 6) freezing; 7) unfreezing in a water bath after freezing, performing recovery processing for once or twice and then inducing plantlets; 8) inducing plantlets and 9) virus detection. The viruses of virus-free seedlings Gisela VI obtained by detoxifying prunus necrotic ring-spot viruses of sweet cherry rootstocks in the combination of thermal treatment and super-freezing therapy can be removed completely; stem tips can be easily peeled off; and the efficient detoxification method has wide application values in promoting healthy and sustainable development of sweet cherry industries.

The present invention provides three kinds of heat tolerant asafetida mushroom nutants, including asafetida mushroom Qinggeli No. 1 CGMCC 1761, asafetida mushroom Qinggeli No. 2 CGMCC 1762 and asafetida mushroom Qinggeli No. 3 CGMCC 1763, obtained through ion beam mutagenesis process with Qinghe wild asafetida mushroom. The process of cultivating asafetida mushroom with these heat tolerant asafetida mushroom nutants includes the steps of slant cultivation, seed bottle and / or fermenting tank cultivation, mushroom bag cultivation and mushroom outputting cultivation. Preferably, in the mushroom outputting cultivation step, water solution comprising alpha-naphthylacetic acid 0.0025 wt%, giberellin 0.0025 wt% triacontanol 0.025 wt% and sterilized water for the rest is injected into the bag. The heat tolerant asafetida mushroom nutants have obviously higher growth temperature than wild asafetida mushroom, and this facilitates the production of asafetida mushroom.

The invention discloses a tissue culture and rapid propagation method of oriental cherry. The method comprises the following steps: obtaining an oriental cherry embryo explant, performing induction culture on the oriental cherry embryo explant, performing multiplication culture on oriental cherry cluster buds, performing rooting culture on the oriental cherry, and hardening and transplanting oriental cherry tissue culture seedlings, wherein the explant is treated according to the method disclosed by the invention during induction culture; and by using a culture medium prepared by the method disclosed by the invention during induction culture (MS + 6-BA1.0-2.0mg / L + NAA or IBA0.1-0.2mg / L + agar 6g / L + sucrose 30g / L), multiplication culture (MS +6-BA2.0mg / L + IBA0.05mg / L + NAA0.05mg / L + agar6g / L + sucrose 30g / L) and rooting culture (1 / 2MS + IBA0.8mg / L + NAA0.1mg / L + AC0.5g / L + agar 6g / L + sucrose 30g / L), a large number of strong and stress-resistant seedlings with high growth vigor andeasiness in rooting can be obtained in a short time, and the seedlings are uniform in growth vigor, high in quality and easy in cultivation management. The tissue culture seedlings obtained by the method disclosed by the invention are high in adaptability to a damp and hot environment, the stems are upright, the production cycle is shortened by 50-60 days, and the yield is increased by 30-50%.

The invention discloses a preprocessing method and manufacture method for Abelia macrotera (Graebn. et Buchw.) Rehd. bean jelly, and relates to the technical field of Abelia macrotera (Graebn. et Buchw.) Rehd. processing. The preprocessing method comprises the following steps of: S101: collecting Abelia macrotera (Graebn. et Buchw.) Rehd.; S102: cleaning collected Abelia macrotera (Graebn. et Buchw.) Rehd. by running water, and flatly laying the Abelia macrotera (Graebn. et Buchw.) Rehd. on a clean place to be dried in the sun for 1-1.5 h; S103: conducting drying processing: laying the above processed Abelia macrotera (Graebn. et Buchw.) Rehd. in a drying oven tray, drying the tray in a drying oven at a temperature of 115-130 DEG C for 10-15 min, and then, cooling to 50-65 DEG C until theAbelia macrotera (Graebn. et Buchw.) Rehd. is dry; and S104: smashing: smashing the dried Abelia macrotera (Graebn. et Buchw.) Rehd. in an ultra-fine powder pulverizer, and sieving by a sieve of 200-400 meshes after the Abelia macrotera (Graebn. et Buchw.) Rehd. is smashed so as to obtain Abelia macrotera (Graebn. et Buchw.) Rehd. ultra-fine powders.

A method for preparing fermented mustard food is as below: washing dried mustard with water, draining the mustard, adding salt and a pickling liquid with lactic acid bacteria to a total of 1*10<6> CFU / mL and a salt content of 4% to 6% into the mustard, and conducting fully mixing, compacting, and sealed fermentation to obtain the fermented mustard food. The method uses the dried mustard as a raw material, realizes annual production, shortens fermentation time and improves the production efficiency; the addition of the pickling liquid can provide a large amount of probiotics, inhibit the growthof the microbes during the fermentation, reduce the nitrite content and improve the safety of the fermented mustard product; and the method can also produce the fermented mustard food with fermentedester flavors to improve the flavor quality of the mustard. The fermented mustard food produced by the invention has ester fragrance and hot and sour taste, rich dietary fiber and a large number of beneficial lactic acid bacteria, and has various functions of improving gastrointestinal functions, preventing constipation, inhibiting cholesterol absorption, lowering blood fat, and resisting aging.

The present invention relates to plant cultivation technology, and is greenhouse asparagus cultivating process. The green house asparagus cultivating process includes the steps of transplanting asparagus seedling in greenhouse, picking up spring asparagus in January to March, leaving spring stubble in April, picking up summer asparagus in May to August, leaving autumn stubble in September, picking up autumn asparagus in October, applying base fertilizer for three times before picking up, applying organic fertilizer and top dressing properly, sterilizing soil, and preventing and controlling diseases and pests. The present invention has high asparagus yield, less diseases and pests, less pollution to asparagus and high asparagus quality.

The invention relates to a method for producing Coprinus comatus by using the anniversary of the soil hole. The method for producing Coprinus comatus by using the anniversary of the soil hole is to use the wine tank as the cultivation raw material and the soil hole as the cultivation environment. The cultivation mode of "inside mushrooming" is realized through 8 steps of soil hole excavation, strain production method, cultivation material formula, cultivation material fermentation treatment, mushroom bag production, mushroom bag growth, soil covering and mushroom fruiting management. The invention uses a manually excavated soil hole as a production site, and provides a cultivation mode of "growing fungus in the hole and fruiting in the hole". Coprinus comatus does not need refrigeration or heating equipment, and it can meet the growth of Coprinus comatus fruiting bodies in a natural state, realizing the annual production of Coprinus comatus; using new cultivation raw material distiller's grains, shortening the processing time of cultivation materials, reducing labor costs, and reducing production costs .

The invention relates to the technical field of edible fungus planting, and discloses a shiitake mushroom greenhouse capable of achieving annual fruiting. The shiitake mushroom greenhouse comprises asteel structure greenhouse framework composed of longitudinal steel pipes and transverse steel pipes and a roller shutter machine, the steel structure framework is covered with a layer of films, the films are covered with a layer of cotton quilts, and the lower ends of the films and the cotton quilts are connected with the roller shutter machine; stand columns are vertically arranged in the greenhouse framework at certain intervals, the stand columns are connected with the greenhouse framework, a mounting frame is arranged at the top in the greenhouse framework, a plurality of spraying water pipes are fixed to the mounting frame, and spraying heads are arranged on the spraying water pipes at intervals; and a plurality of rows of fruiting frames are arranged in the greenhouse framework, anda water collecting ditch is formed in the lower portion of the greenhouse framework. According to the shiitake mushroom greenhouse, the outer layers of the greenhouse films are covered with the cotton quilts, so that a roof can be increased, the heat preservation and heat insulation effects are reasonably achieved, and the cotton quilts are completely put down in summer to achieve the shading andheat insulation effects.

The invention discloses a method for cultivating mesembryanthemum crystallinum. The method comprises the following steps of (1) sowing, namely before sowing, sieving a seedling substrate, watering the substrate by using a water spraying pipe with the proper humidity of 85%, and sowing mesembryanthemum crystallinum seeds into the substrate of pure vermiculite; (2) germinating, namely after sowing, keeping the humidity of the seedling substrate at 80% and the temperature at 26-28 DEG C, and after sowing for about 4-6 days, enabling the mesembryanthemum crystallinum to emerge completely; and (3) seedling management, namely controlling the seedling environment temperature to be 22-28 DEG C and the environment humidity to be 65-75% after seedling emergence, and after seedlings grow to have three main leaves, transplanting and planting the seedlings into a field or a potted plant container. According to the method for cultivating the mesembryanthemum crystallinum, the bottleneck of a mesembryanthemum crystallinum seedling raising technology in an adverse environment is broken through, annual production of potted mesembryanthemum crystallinum is achieved, and supply and demand imbalance caused by seasonal shortage of the mesembryanthemum crystallinum market is solved.

The invention discloses a method for producing catharanthus roseus alkaloid. The method comprises the following steps: firstly, dedifferentiation culture is performed on the explant of the catharanthus roseus to get the callus of the catharanthus roseus, and through succesive transfer culture, loose and quick-growing cell line is obtained; secondly, the cell line of the callus of the catharanthusroseus obtained in the step one is processed through inducing the culture in basal culture medium provided with colchicines to get polyploidy cell line, and then expanding culture is performed to geta large amount of catharanthus roseus polyploidy cells; thirdly, the catharanthus roseus polyploidy cells are inoculated into a deffined medium to get cells being rich ofcatharanthus roseus secondarymetabolites. The method of the invention produces the catharanthine with the catharanthus roseus, the industrial production of the catharanthine can be firmly realized, the production cost is low, theculture period is short, the production is not influenced by natural environment and climate, and the year-round production can be realized.