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Method of obtaining saline cistanche phenylethanol glycoside kind compound using bio-conversion technique

A technology of biotransformation and cistanche, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., to achieve the effect of short cultivation period and low production cost

Inactive Publication Date: 2004-12-22
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the artificial cultivation of Cistanche has been listed as a key project in the "Tenth Five-Year Plan", it is regrettable that it is still difficult to meet the market demand through artificial cultivation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Addition of two amino acid precursors for biotransformation

[0016] (1) In the improved MS medium, add 20-50 g of sucrose or glucose, 0.1-4 mg of indole acetic acid (IAA) and 0.1-4 mg of 6-benzylaminopurine (BA) per liter of culture medium to make a basic medium .

[0017] (2) Add 50-500 mg of phenylalanine and 50-400 mg of tyrosine per liter of culture medium to the basic medium, then adjust the pH value to 5.5-7.0 with acid or alkali to make a synthetic medium, Sterilized at 125°C under 0.1Mpa pressure and cooled before use.

[0018] (3) Inoculate the Cistanche deserticola callus cells on the synthetic medium described in step (2), and the inoculation amount is about 20%. After culturing at 25° C. for one week in the dark, the cells are harvested, freeze-dried, extracted with a solvent to obtain a crude extract, and purified to obtain phenylethanol glycosides.

Embodiment 2

[0019] Example 2: Addition of Cucumber Juice Precursors for Bioconversion

[0020] The cucumber juice in this example can be obtained by the following method: select fresh cucumber, stir and pulverize it, centrifuge at 4000r.p.m, 20°C for 30min, take the supernatant and filter it, and store it at 4°C for later use.

[0021] (1) In the improved MS medium, 20-50 g of sucrose or glucose, 0.1-4 mg of indole acetic acid (IAA) and 0.1-4 mg of kinetin were added per liter of culture medium to make a basic medium.

[0022] (2) Add 40 to 200 ml of cucumber juice per liter of culture medium to the basic medium, then adjust the pH value to 5.5 to 7.0 with acid or alkali to make a synthetic medium, and sterilize at 125°C under a pressure of 0.1Mpa After cooling, it is ready to use.

[0023] (3) Inoculate the Cistanche deserticola callus cells on the synthetic medium described in step (2), and the inoculation amount is about 30%. After culturing at 25° C. for one week in the dark, the ce...

Embodiment 3

[0024] Example 3: Addition of caffeic acid precursor for biotransformation

[0025] (1) In the improved MS medium, add 20-50 g of sucrose or glucose, 0.1-4 mg of indole acetic acid (IAA) and 0.1-4 mg of 6-benzylaminopurine (BA) per liter of culture medium to make a basic medium .

[0026] (2) Add 5 to 100 ml of caffeic acid per liter of culture medium to the basic medium, then adjust the pH value to 5.5 to 7.0 with acid or alkali to make a synthetic medium, and sterilize at 125°C under a pressure of 0.1Mpa After cooling, it is ready to use.

[0027] (3) Inoculate the Cistanche deserticola callus cells on the synthetic medium described in step (2), and the inoculation amount is about 50%. After culturing at 25° C. for one week in the dark, the cells are harvested, freeze-dried, extracted with a solvent to obtain a crude extract, and purified to obtain phenylethanol glycosides.

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PUM

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Abstract

A procss for preparing phenylethanoid glycoside from cistanche by bioconversion technique includes adding cane sugar or glucose, indoleacetic acid, and cytokinin to the improved culture medium MS, adding precursor, regulating pH 5.5-7.0 to obtain synthetic culture medium, disinfecting, cooling, inoculating the calli of cistanche to the synthetic culture medium, oscillating culture in dark condition, collecting the cells, freeze drying, extracting in solvent and purifying.

Description

technical field [0001] The invention relates to a method for obtaining secondary metabolites of Cistanche deserticola, in particular to a method for obtaining phenylethanoid glycosides of Cistanche deserticola by utilizing the biotransformation technology of Cistanche deserticola cells. Background technique [0002] Cistanche (Cistanche) is a perennial parasitic herb of the family Cistanche. It is native to the saline-alkali land, dry river ditch or Gobi Desert in Inner Mongolia, Shaanxi, Gansu, Ningxia and Xinjiang in my country. It mainly parasitizes on the roots of plants such as red salix, salt claw, leaf-bearing salt claw, pearl and Siberian thorn. Due to the harsh environment of its habitat and slow growth rate, production is very limited. Cistanche deserticola is a traditional Chinese medicine that has the functions of nourishing the kidney, benefiting the essence, strengthening the tendons and strengthening the marrow. Long-term service can strengthen the body and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C12N5/04C12P19/44
Inventor 郭志刚
Owner TSINGHUA UNIV
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