Tissue culture and rapid propagation method of oriental cherry
A technology for tissue culture and cherry blossoms, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of cost reduction, slow propagation speed of mountain cherry blossoms, and high production costs
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Embodiment 1
[0029] Embodiment 1: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:
[0030] (1) Obtaining the explants of wild cherry blossoms:
[0031] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...
Embodiment 2
[0040] Embodiment 2: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:
[0041] (1) Obtaining the explants of wild cherry blossoms:
[0042] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...
Embodiment 3
[0051] Embodiment 3: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:
[0052] (1) Obtaining the explants of wild cherry blossoms:
[0053] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...
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