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Tissue culture and rapid propagation method of oriental cherry

A technology for tissue culture and cherry blossoms, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of cost reduction, slow propagation speed of mountain cherry blossoms, and high production costs

Active Publication Date: 2020-10-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a method for tissue culture and rapid propagation of wild cherry to solve the problems of slow propagation speed and high production cost of wild cherry, so as to shorten the production cycle and reduce the cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:

[0030] (1) Obtaining the explants of wild cherry blossoms:

[0031] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...

Embodiment 2

[0040] Embodiment 2: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:

[0041] (1) Obtaining the explants of wild cherry blossoms:

[0042] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...

Embodiment 3

[0051] Embodiment 3: the steps of a kind of mountain cherry tissue culture and rapid propagation method are:

[0052] (1) Obtaining the explants of wild cherry blossoms:

[0053] Collect the fruits of the mountain cherry blossoms that were fruitful in the same year. During the fruiting period in late spring, select plants that grow robustly and are free of diseases and insects. Collect mature, complete and insect-free fruits, bring them back indoors, wash them with water, remove the pulp, and place them in a cool and ventilated place to dry . After drying, remove the seed husks, soak them in 75% alcohol for 30s on the ultra-clean workbench, rinse them twice with sterile water, soak them with 2% sodium hypochlorite for 5 minutes, and rinse them with sterile water for more than 5 times. Soak overnight in sterile water. The next day, put the sterilized materials into a sterile petri dish, blot the excess water with filter paper, cut the seeds with a scalpel, remove the cotyledo...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method of oriental cherry. The method comprises the following steps: obtaining an oriental cherry embryo explant, performing induction culture on the oriental cherry embryo explant, performing multiplication culture on oriental cherry cluster buds, performing rooting culture on the oriental cherry, and hardening and transplanting oriental cherry tissue culture seedlings, wherein the explant is treated according to the method disclosed by the invention during induction culture; and by using a culture medium prepared by the method disclosed by the invention during induction culture (MS + 6-BA1.0-2.0mg / L + NAA or IBA0.1-0.2mg / L + agar 6g / L + sucrose 30g / L), multiplication culture (MS +6-BA2.0mg / L + IBA0.05mg / L + NAA0.05mg / L + agar6g / L + sucrose 30g / L) and rooting culture (1 / 2MS + IBA0.8mg / L + NAA0.1mg / L + AC0.5g / L + agar 6g / L + sucrose 30g / L), a large number of strong and stress-resistant seedlings with high growth vigor andeasiness in rooting can be obtained in a short time, and the seedlings are uniform in growth vigor, high in quality and easy in cultivation management. The tissue culture seedlings obtained by the method disclosed by the invention are high in adaptability to a damp and hot environment, the stems are upright, the production cycle is shortened by 50-60 days, and the yield is increased by 30-50%.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of wild cherry, and relates to a tissue culture and rapid propagation method of wild cherry. Background technique [0002] Cherry blossoms are generally used to refer to the species of cherry plants used for viewing or the flowers of the plants of the genus Sakura in the Rosaceae Li subfamily. The traditional propagation methods of cherry blossoms are mainly cutting and grafting, but on the one hand, these propagation methods require a large number of mother plants, and on the other hand, they also need to spend a lot of manpower, material resources, financial resources, time, etc., and the propagation coefficient is low, and the seedlings are slow to grow. The main propagation method of mountain cherry is sowing, but because the seeds have a hard outer testa, sowing is not easy to germinate, and cuttings and grafting of mountain cherry are difficult to survive. With the wide application o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 欧静徐晨捷
Owner GUIZHOU UNIV
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