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Liquid culture medium for efficiently producing Piriformospora indica

A technology of piriformis indica and liquid culture medium, which is applied in the field of plant cell and tissue culture biology, can solve the problems of simple fungal medium composition, slow growth of strains, and affecting the quality of strains, so as to improve the speed and quality of reproduction and reduce the cost , the effect of efficient and rapid reproduction

Inactive Publication Date: 2012-08-22
YANGTZE UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the prior art, due to the slow growth and low yield of the bacterial strain, and the simple composition of the fungal culture medium, which affects the quality of the bacterial strain, it is of great significance to screen a liquid medium suitable for the efficient production of Pyromorpha indica

Method used

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with specific examples.

[0011] 1. Preparation of liquid medium:

[0012] Add 80g of peeled potatoes to 1L of water and boil. After boiling for 20 minutes, filter the supernatant, add 15g of sucrose to the supernatant; 35ml of winter melon juice, 25ml of cucumber juice, 15ml of tomato juice, 30ml of carrot juice, 15ml of leek juice and 20ml of soil extract; adjust the pH to 6.5-7.0 to obtain a liquid medium.

[0013] 2. Inoculation: When the medium is cooled to 30±2°C, inoculate 0.2-0.5cm 2 Bacteria.

[0014] 3. Cultivation: Set the shaking table culture temperature at 30±2°C and 100rph. After three days of cultivation, pick a single colony and continue to expand the cultivation.

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Abstract

The invention relates to a liquid culture medium for efficiently producing Piriformospora indica and belongs to the technical field of plant cell and tissue culture biology. The liquid culture medium is characterized in that a preparation method for the liquid culture medium comprises the following steps of: adding 80-100g of potato in 1L of water for boiling and filtering to obtain supernate; then sequentially adding 15-20g of cane sugar, 30-35ml of white gourd juice, 20-25ml of cucumber juice, 10-15ml of tomato juice, 20-30 ml of carrot juice, 10-15ml of leek juice and 20-25ml of soil water extract in the supernate; and adjusting the PH value to 6.5-7.0. According to the liquid culture medium disclosed by the invention, high-efficiency and quick reproduction of Piriformospora indica bacterium can be realized; the reproduction speed and quality of strains are improved; and the liquid culture medium is convenient in material accessibility, is low in cost and has a favorable economic benefit.

Description

Technical field: [0001] The invention relates to a liquid culture medium for efficiently producing Pyromorpha indica, which belongs to the field of plant cell and tissue culture biotechnology. Background technique: [0002] Microbial fertilizer, also known as microbial inoculant, is a living product. The development and utilization of microbial resources should first be the development and utilization of microbial organisms. The application of microbial fertilizer is one of the ways to develop and utilize microbial organisms. With the rise and development of ecological agriculture, the position and function of microbial fertilizer will increasingly attract people's attention. After my country's accession to WTO, in order to improve the competitiveness of agricultural products, farmers in our country have an increasing demand for microbial fertilizers. Pyromorpha indica is a mycorrhizal fungus that can be purely cultured. This fungus can promote the growth of 28 species of...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 董社琴杨亚珍叶开温曹勋
Owner YANGTZE UNIVERSITY
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