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45 results about "Piriformospora" patented technology
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Piriformospora is a fungal genus of the order Sebacinales. It is a monotypic genus, containing the single endophytic root-colonising species Piriformospora indica, discovered from orchid plants in the Thar desert in Rajasthan, India by Prof. Ajit Verma and group, School of Life Sciences, Jawaharlal Nehru University, New Delhi. The fungus has typical pear-shaped chlamydospores, and thus, named as P. indica . Unlike mycorrhizal fungi, which cannot be cultured axenically, P. indica can be easily grown on various substrates. It has been found to promote plant growth during its mutualistic symbiotic relationship with a wide variety of plants. Experiments have shown that P. indica increases the resistance of colonized plants against fungal pathogens. It has also been found in experiments with barley that P. indica-inoculated plants are tolerant to salt stress and more resistant to root pathogens. P. indica-infested roots also show antioxidant capacity. The fungus also induces systemic disease resistance in plants. P. indica was found to require host cell death for proliferation during mutualistic symbiosis in barley. Its genome has been sequenced and was published in 2011.
Method for producing biological bacterial fertilizer by taking waste substances as raw material
InactiveCN107056436AReduce total usageIncrease productionBio-organic fraction processingBioloigcal waste fertilisersPiriformosporaBacillus megaterium
The invention discloses a method for producing a biological bacterial fertilizer by taking waste substances as a raw material, belongs to the technical field of biological bacterial fertilizers, and aims to develop a biological bacterial fertilizer which has the effects of resisting cold, drought, waterlogging and diseases and increasing yield by decomposing and fermenting paper mill garbage and urban domestic garbage through the specific functions of a piriformospora indica bacterial strain and bacillus subtilis serving as natural plant growth regulating agents. The technical route comprises the following steps: treating waste rice hull produced in a paper mill and cellulose substances such as straw, and preparing a culture medium together with leftover dry powder; inoculating a fermentation liquor mixture of cellulose degradation bacteria, such as trichoderma, bacillus megaterium, potassium bacteria and the piriformospora indica bacterial strain; fermenting to obtain the liquid bacterial fertilizer. By adopting the method, the current three problems of effective utilization of recyclable resources such as straw and rice hull, pollution abatement of paper mills and urban domestic garbage disposal are solved; meanwhile, the biological bacterial fertilizer has the advantages of improvement on the yield and quality of various crops, low production cost, remarkable fertilizer effect, improvement on the stress resistance of the crops, and very remarkable economic benefit.
Owner:XINZHOU TEACHERS UNIV
Method for reducing cadmium content in tobacco leaves
InactiveCN103891502AImprove nutritional statusPromote growthFungiMicroorganism based processesPiriformosporaNicotiana tabacum
Provided is a method for reducing the cadmium content in tobacco leaves. The method comprises the specific steps that (1) nanometer material zinc oxide is used for replacing ZnSO4.7H2O in original Kafer culture media, and 10 mg of nanometer material zinc oxide is added into 1 liter of culture media; (2) activated Piriformospora indica is inoculated to the liquid Kafer culture media in the step (1) for culture, then mycelia are collected, Piriformospora indica mycelium liquid is made and poured to roots of tobacco seedlings, and root-irrigation is conducted so that the Piriformospora indica can be colonized to the roots of the tobacco seedlings; (3) the tobacco seedlings are transplanted after the Piriformospora indica is colonized, and conventional water and fertilizer management are conducted so that the tobacco seedlings can grow normally. The growth status of the tobacco seedlings can be improved in the method, the amount of cadmium moving to overground parts of the tobacco seedlings from root systems is reduced, heavy metal residue on the tobacco leaves is reduced, and the method is easy to master, low in cost, simple, practical and environmentally friendly, and can not case secondary pollution.
Owner:CHINA TOBACCO GUIZHOU IND
Method for cultivating oncidium
InactiveCN104221854AReduce the rate of transplanting diseasesImprove stress resistanceHorticulture methodsPlant tissue culturePiriformosporaBottle
The invention relates to the field of agricultural biotechnology and provides a method for cultivating oncidium, aiming at solving the problems such as rooting difficulty, weak reproductive capacity, low growth speed, long growth period, severe diseases in seedling hardening and transplanting stages, low bottle-out survival rate and weak stress resistance existing during oncidium production. The method for cultivating oncidium comprises the following steps: (1) culturing oncidium tissue culture seedlings by an MS culture medium; (2) culturing Piriformospora indica fungi by a solid PDA culture medium; (3) co-culturing the oncidium and the Piriformospora indica fungi; and (4) performing bottle-out cultivation on the oncidium tissue culture seedlings. According to the cultivation method disclosed by the invention, the disease rate during oncidium bottle-out seedling transplanting is reduced, and the stress resistance is improved.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST +1
Method for reducing lead content of tobacco leaves
ActiveCN103858685AImprove nutritional statusPromote growthFungiMicroorganism based processesPiriformosporaNicotiana tabacum
The invention provides a method for reducing lead content of tobacco leaves. According to the method, parasitic piriformospora indica at the root of tobacco is utilized to reduce heavy metal lead residues in tobacco. The method comprises the following specific steps: (1) by use of utilizing nano material ZnO to replace ZnSO4.7H2O in an original Kafer culture medium, adding 10mg of nano material ZnO into per litre of the culture medium; (2) inoculating the activated piriformospora indica into the liquid Kafer culture medium obtained in the step (1) for culturing, then collecting mycelia, preparing a piriformospora indica mycelium solution, then pouring the piriformospora indica mycelium solution to the root of a tobacco seedling, and performing root-irrigation treatment so as to colonize the piriformospora indica at the root of the tobacco seedling; (3) after performing piriformospora indica colonization, transplanting the tobacco seedling into a large field, and then performing routine water and fertilizer management so as to enable the tobacco to grow normally. The method can be used for improving the tobacco growth condition, reducing the transfer of the heavy metal lead from a root system to the overground part of the tobacco and reducing the heavy metal lead residues, and is easy to master, relatively low in cost, simple and practical, environment-friendly, and incapable of causing secondary pollution.
Owner:CHINA TOBACCO GUIZHOU IND
Method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii
ActiveCN107155896ASimple multiplicationSimple preparation processPlant tissue cultureHorticulture methodsPiriformosporaPlant roots
The invention provides a method for promoting rooting of tissue-cultured transplant seedlings of Gerbera jamesonii; irrigating with Piriformospora indica is performed, and Piriformospora indica is used to promote rooting of the tissue-cultured transplant seedlings of Gerbera jamesonii, improve the roots and promote the growth of plants; in addition, the method may also solve the problems of regenerated plant root weakness, low growth speed, seedling weakness and the like due to multiple subculture, and is significant to promoting the commodity value of Piriformospora indica plants. The method has simple steps, is highly operable and has significant effect.
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for lowering content of chromium in tobacco
ActiveCN103875398AImprove nutritional statusPromote growthHorticulture methodsFertilizer mixturesPiriformosporaCulture mediums
The invention discloses a method for lowering the content of chromium in tobacco. According to the method for lowering the content of chromium in the tobacco, piriformospora indica is grown on the root of the tobacco in a parasitic manner so as to reduce heavy metal chromium residuals in the tobacco. The method for lowering the content of chromium in the tobacco specifically comprises the steps that (1) ZnSO4 7 H2O in an original Kafer culture medium is replaced by nanometer material zinc oxide, and 10 mg of nanometer material zinc oxide is added to each liter of culture medium; (2) activated Piriformospora indica is inoculated to the Kafer culture medium obtained in the step (1) to be cultured, then mycelia are collected, piriformospora indica hypha liquid is prepared, the prepared piriformospora indica hypha liquid is then poured to the root of a tobacco seedling, and root-irrigation treatment is conducted, so that the piriformospora indica is colonized on the root of the tobacco seedling; (3) after the piriformospora indica is colonized on the root of the tobacco seedling, the tobacco seedling is transplanted to a land, and then conventional water and fertilizer management is conducted to make the tobacco seedling grow normally. According to the method for lowering the content of chromium in the tobacco, the growth condition of the tobacco can be improved, the amount of heavy metal chromium which transfers from the root of the tobacco to the overground part of the tobacco is reduced, the heavy metal chromium residuals in the tobacco are reduced, the method is easy to master, the cost is low, the method is easy and practical, environmental friendliness is achieved, and secondary pollution can not be caused.
Owner:CHINA TOBACCO GUIZHOU IND
Method for improving salt stress tolerance of medicago truncatula
InactiveCN105850535APromote early germinationImprove germination rateSeed and root treatmentHorticulture methodsSporePiriformospora
The invention relates to a method for improving salt stress tolerance of medicago truncatula. The method comprises the steps: (1) preparing a solid CM complete medium for piriformospora indica; (2) culturing the piriformospora indica and collecting a spore suspension; (3) inoculating the piriformospora indica to the medicago truncatula; and (4) transplanting medicago truncatula seedlings and carrying out later-stage management. According to the method, the piriformospora indica spore suspension is adopted to soak seeds in advance, so that the piriformospora indica can be parasitized to surfaces of the medicago truncatula seeds, the seeds are promoted to germinate ahead of time, and the germination percentage of the seeds is remarkably increased (increased to 95.0% from the original 80.0%); the biological yield of the medicago truncatula can be remarkably increased; and through parasitizing the piriformospora indica to roots of the medicago truncatula, the accumulation of sodium ions in the roots of the medicago truncatula can be lowered, and thus the salt stress tolerance of the medicago truncatula is improved.
Owner:HEBEI UNIV OF TECH
Method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil
InactiveCN106171101ASimple cultivation conditionsPromote growthSoil-working methodsPiriformosporaSpore
The invention discloses a method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil. The method includes following steps: (1), culturing piriformospora indica, and collecting spore suspension; (2), cultivating seeds of medicago sativa; (3), inoculating piriformospora indica to medicago sativa. The piriformospora indica spore suspension is adopted to irrigate roots of medicago sativa, piriformospora indica is planted at the roots of medicago sativa, mycorrhizal symbionts formed by plants and endophytic fungi can promote growing of medicago sativa, bioactivity and fertility of soil are improved, petroleum in the soil can be effectively degraded, and degradation rate can reach 78%.
Owner:HEBEI UNIV OF TECH
Method for reducing cadmium content of overground part of paddy rice
InactiveCN103858719AGrow vigorouslyImprove colonization rateHorticulture methodsRice cultivationPiriformosporaCulture mediums
The invention provides a method for reducing cadmium content of an overground part of paddy rice. The method comprises the following specific steps: (1) by use of utilizing nano material ZnO to replace ZnSO4.7H2O in an original Kafer culture medium, adding 10mg of nano material ZnO into per litre of the culture medium; (2) inoculating the activated piriformospora indica into the liquid Kafer culture medium obtained in the step (1) for culturing, then collecting mycelia, preparing a piriformospora indica mycelium solution, then pouring the piriformospora indica mycelium solution to the root of a paddy rice seedling, and performing root-irrigation treatment so as to colonize the piriformospora indica at the root of the paddy rice seedling; (3) after performing piriformospora indica colonization, transplanting the paddy rice into a large field, and then performing routine water and fertilizer management so as to enable the paddy rice to grow normally. The method is easy to master, relatively low in cost, simple and practical, environment-friendly, incapable of causing secondary pollution, applicable to production of chemical-free rice, not only applicable to planting of rice in heavy metal contaminated soil, and also applicable to uncontaminated soil.
Owner:ZHEJIANG UNIV
Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos
ActiveCN105794455AImprove prevention and controlPlay a synergistic roleGrowth substratesCulture mediaFungicidePiriformospora
The invention discloses a method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos. The method includes the following steps that 1, tobacco seeds are sowed in a seedling raising substrate mixed with piriformospora indica hyphae, and seedlings are transplanted to fields after seedling development; 2, before transplanting, holes for tobacco seedling transplanting are irrigated with a Zhongshengmycin solution; root-irrigation treatment is conducted on tobacco plants by using the Zhongshengmycin solution. The piriformospora indica which is adopted by the method and is not inhibited by the Zhongshengmycin can combine with the fungicide Zhongshengmycin for usage and is applied to prevention control of the bacterial wilt of the tobaccos. Compared with the prevention and control effect of the separately used piriformospora indica or the Zhongshengmycin, the jointly used piriformospora indica and Zhongshengmycin can play a synergic effect and remarkably improve the prevention and control effect on the bacterial wilt of the tobaccos.
Owner:ZHEJIANG UNIV
Nano-zinc oxide-based culture medium capable of promoting microbial growth
InactiveCN103898040AFast growth rateGood growth promoting effectFungiMicroorganism based processesPiriformosporaNiacin
The invention discloses a nano-zinc oxide-based culture medium capable of promoting growth of piriformospora indica. Every liter of culture medium comprises 2.00g of peptone, 1.00g of yeast extract, 1.00g of casein hydrolysate, 0.60g of NaNO3, 0.52g of KCl, 0.52g of MgSO4.7H2O, 1.52g of KH2PO4, 5.00-10.00mg of ZnO, 10mg of H3BO3, 5mg of MnSO4.H2O, 1.6mg of CoCl.5H2O, 1.6mg of CuSO4.5H2O, 1.1mg of (NH4) 6MO7O24, 1.3mg of FeSO4, 1.8mg of ethylene diamine tetraacetic acid (EDTA), 0.10mg of thiamine, 0.04mg of glycine, 0.01mg of niacin and 0.01mg of pyridoxine. Compared with the original culture medium, the nano-zinc oxide-based culture medium has the advantage that the growth speed of the piriformospora indica is obviously faster; the fresh weight of thallus cultured by using the medium is respectively increased by 36.5% and 55.8% compared with the fresh weights of control groups; the dry weight of the thallus cultured by using the medium is respectively increased by 19% and 26.2% compared with the dry weights of control groups; and the bacterial colony is more compact and smoother than the original bacterial colony. The method provided by the invention is simple, uses easily available materials and is low in cost and good in economic benefit.
Owner:ZHEJIANG UNIV
Piriformospora indica fermentation method and response surface optimization method used by same
InactiveCN103290058AIncrease productionEmission reductionMicroorganism based processesFermentationPiriformosporaDesign–Expert
The invention discloses a piriformospora indica fermentation method. The method comprises the following steps: by taking a PDB culture medium as a culture solution, regulating the pH of the culture solution to be 5.0-5.1 by using a HCl solution with the concentration of 1mol / L, and performing culture fermentation on piriformospora indica at the temperature of 27.5-28.5 DEG C for 14-15 days, so as to obtain the fermentation liquor. The invention also discloses a method for performing response surface optimization in the piriformospora indica fermentation process. The method comprises the following steps: setting the three variables including the pH, the concentration of the PDB culture medium and the culture time; optimizing according to the Box-Behnken design principle by utilizing design expert software. According to the method, the yield of secondary metabolites can be increased, and the consumption of nutritional ingredients and culture media is reduced.
Owner:ZHEJIANG UNIV
Method for promoting rooting and root strengthening of banana tissue culture seedlings
InactiveCN107135952AEasy to trainEasy to operateFungiMicroorganism based processesPiriformosporaSeedling
The invention provides a method for promoting rooting and root strengthening of banana tissue culture seedlings. The method comprises the following steps: cultivation of Piriformospora indica, preparation of a banana rooting medium, inoculation for co-culture of Piriformospora indica and banana tissue culture seedlings, and the like. Colonization of Piriformospora indica is used for promoting rooting of banana tissue culture seedlings, the method has simple operation, rooting period of tissue culture seedlings is shortened, and the method can substantially promote rooting of the banana tissue culture seedlings with a certain root strengthening effect. The method has good operationality and substantial effects.
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for improving resistance of plants to bensulfuron methyl serving as herbicide by using Piriformospora indica
ActiveCN107926549AIncrease resistanceEnsure safetyFungiMicroorganism based processesPiriformosporaNicotiana tabacum
The invention discloses an application of Piriformospora indica to improvement of the resistance of plants to bensulfuron methyl serving as a herbicide and belongs to the technical field of crop planting. The Piriformospora indica is not inhibited by bensulfuron methyl, and the established Piriformospora indica-crop co-culture system is capable of remarkably improving the resistance of crops to bensulfuron methyl and reducing phytotoxicity; and after the Piriformospora indica serving as a beneficial endophytic fungus is colonized into the crops, no side effects are generated, and meanwhile, the environment safety is guaranteed. The crops are rice, rapes, sunflowers, tobaccos, wheat and maize. The invention further provides a method for improving the resistance of rice to bensulfuron methylserving as a herbicide. The method comprises the steps: co-culturing rice seedlings in a rice culture solution containing Piriformospora indica to obtain rice seedlings in which the Piriformospora indica is colonized, and then, transplanting the rice seedlings in which the Piriformospora indica is colonized to a farmland, and culturing the rice seedlings until harvesting rice.
Owner:ZHEJIANG UNIV
Nutrient solution for hydroponics plants capable of continuously releasing negative ions
InactiveCN105754880AExtend your lifePromote growthFungiMicroorganism based processesPiriformosporaNutrient solution
The invention relates to nutrient solution for hydroponics plants capable of releasing negative ions, and belongs to the field of functional plant hydroponics.The negative ions can be continuously released by the hydroponics plants such as tequila by the aid of the nutrient solution, and the lives of the hydroponics plants can be prolonged by the aid of the nutrient solution.The nutrient solution is improved Hoagland nutrient solution with piriformospora indica fungi.The nutrient solution has the advantages that the hydroponics plants (including the tequila and yucca gloriosa) can be cultivated by the aid of soilless cultivation methods, the nutrient solution can be applied to the hydroponics plants on plant negative ion machines, accordingly, the negative ions can be continuously and efficiently released, growth of the root systems of the hydroponics plants can be promoted, the leaves of the hydroponics plants keep deeply green, the negative ions can be assuredly continuously and efficiently released by the hydroponics plants in the plant negative ion machines, and the lives of the plants can be prolonged.
Owner:FUJIAN AGRI & FORESTRY UNIV
Piriformospora indica microbial agent and preparation method thereof
ActiveCN114107070ASolve the difficulty of sporulationSolve difficult-to-scale applicationsBiocidePlant growth regulatorsBiotechnologyPiriformospora
The invention discloses a piriformospora indica microbial inoculum and a preparation method thereof, the piriformospora indica microbial inoculum comprises a fermentation carrier containing piriformospora indica thalli as an active ingredient, a spore protective agent, a dispersing agent and a spreading agent, the content of piriformospora indica chlamydospore in the fermentation carrier is (1.0-20.0) * 10 < 6 > spores / g, the weight percentage of the dispersing agent and the spreading agent is 1-5%, and the weight percentage of the spreading agent is 1-5%. The weight percentage of the spore protective agent is 0.05 to 0.1 percent. The method successfully solves the problem that piriformospora indica is difficult in spore production, large-scale application and long-term storage, when the seed solution is prepared, soluble starch is adopted as a carbon source, compared with other carbon sources, the growth speed of piriformospora indica hyphae can be remarkably increased, the spore production time is remarkably shortened, the spore production quantity is increased, and the production cost is reduced. Through solid culture with sorghum and cane sugar as fermentation carriers, the storability is improved, the prepared microbial inoculum can be preserved for a long time, the spore activity can be kept, and possibility is provided for large-area production and application.
Owner:天津市农业科学院
Method for improving yield of liquid fermentation products of Piriformospora indica
InactiveCN107841514APromote generationWide variety of sourcesFungiMicroorganism based processesPiriformosporaMetabolite
The invention discloses a method for improving yield of liquid fermentation products of Piriformospora indica. The method comprises the following steps: a Piriformospora indica liquid seed solution isinoculated to a solid culture medium with added exogenous substances for culture, and after vacuum freeze drying and pulverizing are carried out for cultivated products, an exogenous substance solidfermentation culture is obtained; exogenous substances are selected from one or two kinds of fresh rehmannia roots and gardenia fruits; the Piriformospora indica liquid seed solution is inoculated toa liquid fermentation culture medium with the added exogenous substance solid fermentation culture, cultivation is carried out for a period of time, cold shock proteins are added, cultivation is continued for a period of time, and after fermentation is terminated, the fermentation products of Piriformospora indica are obtained. The method can be used for effectively promoting generation of Piriformospora indica metabolites.
Owner:ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
Method for increasing germinating rate of longan seeds under condition of short-time room temperature storage
ActiveCN107810679AEasy to manufactureHigh feasibilityFungiSeed and root treatmentPiriformosporaRoom temperature
The invention belongs to the technical field of seedling culture of longan, and particularly relates to a method for increasing germinating rate of longan seeds under condition of short-time room temperature storage. The method is characterized in that the longan seeds under the condition of short-time room temperature storage are soaked into a piriformospora indica fermenting liquid, and are fetched out for seeding; compared with the longan seeds which are not soaked into the piriformospora indica fermenting liquid, the germinating rate is obviously increased, and the seedling can well grow.The method has the advantages that the preparation of the piriformospora indica fermenting liquid is easy, the feasibility is strong, the operation is simple in the seed treatment process, and the higher application and economic value is realized.
Owner:FUJIAN AGRI & FORESTRY UNIV
Application of piriformospora indica as corn fertilizer
InactiveCN106946591AIncrease biomassIncrease productionFungiMicroorganism based processesPiriformosporaAgricultural science
The invention discloses an application of piriformospora indica as a corn fertilizer, and belongs to the technical field of corn fertilizer. The invention aims to enhance the application of piriformospora indica as a corn fertilizer, and specifically discloses research on application of piriformospora indica as a biological fertilizer for corn plantation. On the basis of previous research, the amount of bacteria is increased. A basis is provided for the application in the field. Through the field tests, the length of corn roots is obvious prolonged, and the corn yield is prominently increased. The piriformospora indica can be made into a green, safe, pollution-free, stress resistant, and growth promoting biological fertilizer, which solves the problems caused by unreasonable application of chemical fertilizers such as soil hardening, environment pollution, and the like, and overcomes the shortages of various biological fertilizers such as limited application range, unstable fertilizing effect, and the like. A novel way is provided for the development of ecological agriculture.
Owner:XINZHOU TEACHERS UNIV
Application of piriformospora indica in regulating drought tolerance of osmanthus fragrans
PendingCN112075233AImprove toleranceEase of mass productionGrowth substratesCulture mediaPiriformosporaPropanedial
The invention discloses an application of piriformospora indica in regulating drought tolerance of osmanthus fragrans. That is, in a cultivation process, the piriformospora indica or a biological agent containing the piriformospora indica is inoculated in osmanthus fragrans seedlings, so that the piriformospora indica and osmanthus fragrans root systems are symbiotic, the drought tolerance of theosmanthus fragrans to soil drought can be stimulated, and the drought tolerance of the osmanthus fragrans is remarkably improved. The normal growth increment can be still maintained under the water supply condition that the maximum field water-holding capacity of the osmanthus fragrans is 15%, meanwhile, the content of soluble sugar and free proline and the activity of POD and SOD enzymes in osmanthus fragrans leaves are remarkably improved, and the content of malondialdehyde is remarkably reduced. Therefore, a new thought and a technical basis are provided for water-saving cultivation of osmanthus fragrans. The piriformospora indica can be rapidly proliferated by utilizing an artificial culture medium, is easy to produce in batches, is further popularized and applied on a large scale, andhas commercial potential advantages.
Owner:YANGTZE UNIVERSITY
A method for promoting the rooting of gerbera tissue culture transplanted seedlings
ActiveCN107155896BSimple multiplicationSimple preparation processHorticulture methodsPlant tissue culturePiriformosporaPlant roots
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for improving root rot resistance of Gerbera jamesonii
ActiveCN108668896AEnhance colonization abilityIncreased rot resistancePlant tissue cultureHorticulture methodsBiotechnologyPiriformospora
The invention provides a method for improving the root rot resistance of Gerbera jamesonii. Gerbera jamesonii colonized with Piriformospora indica is obtained by a root immersion technology to improvethe root rot resistance of Gerbera jamesonii, so the method is of great significance to improve the commercial values of Gerbera jamesonii plants. The method has the advantages of simple steps, strong operability and remarkable effect.
Owner:FUJIAN AGRI & FORESTRY UNIV
A kind of preparation method of Indian piriformis stalk culture medium for cultivating shiitake mushrooms
ActiveCN107216201BImprove clinical outcomesIncrease productionCalcareous fertilisersBio-organic fraction processingBiotechnologyPiriformospora
The invention provides a preparation method for a Piriformospora indica-straw medium used for culturing mushrooms. The preparation method comprises the following steps: (1) inoculating a Kafer medium with Piriformospora indica, carrying out shaking culture at a rotating speed of 120 to 150 r / min and a temperature of 26 to 30 DEG C for 6 to 8 d, carrying out sampling after completion of culture, determining the number of live bacteria and dissolving a culture in distilled water so as to allow the number of live bacteria in every ml of the obtained solution to be 1*10<6>; and (2) sprinkling bacteria liquid obtained in the step (1) and having a bacterium number of 1*10<6> / ml onto the surface of a straw medium, with the inoculation amount of the bacteria liquid being 1 to 15%, and carrying out fermentation at 25 to 37 DEG C for 12 to 24 h so as to obtain the Piriformospora indica-straw medium used for culturing mushrooms. According to the invention, Piriformospora indica is added, and optimal cultivation conditions are selected, so the growth of mushroom rootlets is promoted; and thus, the output of mushrooms is increased.
Owner:YANGTZE UNIVERSITY
Plant aphid resistance inducing preparation and preparation method thereof
InactiveCN111357774APromote growthIncrease productionBiocidePlant growth regulatorsBiotechnologySophorae Radix
The invention belongs to the technical field of planting, and specifically relates to a preparation method for a plant aphid resistance inducing preparation. The preparation method comprises the following steps: inoculating piriformospora indica into a culture medium, and carrying out culturing so as to obtain a piriformospora indica fermentation product; uniformly mixing 20 to 70 parts of the piriformospora indica fermentation product, 20 to 50 parts of jasmonic acid, 10 to 30 parts of oligosaccharide-chain protein, 5 to 8 parts of radix sophorae flavescentis and 60 to 80 parts of a filling agent so as to obtain a mixture; and drying the mixture, and carrying out granulating so as to obtain the plant aphid resistance inducing preparation. The invention also provides the plant aphid resistance inducing preparation. The preparation provided by the invention is simple in preparation steps, environmentally-friendly, free of any pollution and residues, free of side effects, capable of reducing the environmental pollution and overcoming the occurrence of soil-borne diseases, free of the limitation of planting varieties, and wide in application.
Owner:HENAN BAIYU PLANT IMMUNE TECH CO LTD
Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria
PendingCN114868617ALow costHigh survival rate in the wildFlowers cultivationGrowth substratesPiriformosporaPleione bulbocodioides
The invention provides a method for direct sowing and seedling raising of pleione bulbocodioides by utilizing symbiotic bacteria, which comprises the following steps: mixing piriformospora indica into a culture medium, sowing pleione bulbocodioides seeds, and performing symbiotic culture to obtain symbiotic pleione bulbocodioides seedlings. The method is simple and convenient to operate, the symbiotic bacteria and pleione bulbocodioides seeds are directly sown for seedling raising for the first time, after 10-15 days, most of the seeds appear green, after 30 days, a large number of protocorms appear native meristem, after 45 days, the protocorms grow first leaves, after 2 months, seedlings form 2-3 leaves and grow roots, and the seedlings can be transplanted to a nursery garden.
Owner:KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
A kind of method for improving the germination rate and strong seedling of tobacco floating seedlings
InactiveCN104541668BSimple methodImprove germination rateCultivating equipmentsSoilless cultivationPiriformosporaDry weight
Owner:ZHEJIANG UNIV +1
A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid
ActiveCN105766493BImprove the effect of prevention and controlPlay a synergistic rolePlant protectionPiriformosporaNicotiana tabacum
Owner:ZHEJIANG UNIV
Method for improving sporulation of piriformospora indica
ActiveCN113604362AIncrease productionShort training periodFungiMicroorganism based processesBiotechnologyPiriformospora
The invention relates to a method for improving sporulation of piriformospora indica. According to a piriformospora indica culture method, an adopted fermentation culture medium is composed of or prepared from dried potato powder, glucose and water, the content of the dried potato powder in the fermentation culture medium is 10g / L-20g / L, and the content of the glucose in the fermentation culture medium is 10g / L-20g / L. According to the method, piriformospora indica is cultured in the fermentation culture medium composed of the dried potato powder, the glucose and the water, the culture period can be shortened, the spore yield can be increased, the production cost is greatly reduced, and industrial production is facilitated.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI +3
Method for controlling tobacco bacterial wilt through combination of Piriformospora indica with trichloroisocyanuric acid
The invention discloses a method for controlling tobacco bacterial wilt through combination of Piriformospora indica with trichloroisocyanuric acid. The method comprises steps as follows: (1) sowing tobacco seeds in a seedling culture medium mixed with Piriformospora indica mycelia, and seedlings are transplanted to a field; (2) before transplantation, watering holes for tobacco seedling transplantation with a trichloroisocyanuric acid solution, and 10-15 days after transplantation, performing root-irrigation treatment with the trichloroisocyanuric acid solution. The used Piriformospora indica is not inhibited by the pesticide trichloroisocyanuric acid, can be combined with the trichloroisocyanuric acid for use and is applied to control on the tobacco bacterial wilt. The Piriformospora indica and the trichloroisocyanuric acid are combined for use to play a synergistic role in controlling the bacterial wilt, and the control effect is significantly superior to that of the single Piriformospora indica or the single trichloroisocyanuric acid.
Owner:ZHEJIANG UNIV
A method for improving the germination rate of longan seeds stored at room temperature for a short period of time
ActiveCN107810679BEasy to manufactureHigh feasibilityFungiSeed and root treatmentPiriformosporaSeed treatment
Owner:FUJIAN AGRI & FORESTRY UNIV
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