The invention discloses a nano-zinc oxide-based culture medium capable of promoting growth of piriformospora indica. Every liter of culture medium comprises 2.00g of peptone, 1.00g of yeast extract, 1.00g of casein hydrolysate, 0.60g of NaNO3, 0.52g of KCl, 0.52g of MgSO4.7H2O, 1.52g of KH2PO4, 5.00-10.00mg of ZnO, 10mg of H3BO3, 5mg of MnSO4.H2O, 1.6mg of CoCl.5H2O, 1.6mg of CuSO4.5H2O, 1.1mg of (NH4) 6MO7O24, 1.3mg of FeSO4, 1.8mg of ethylene diamine tetraacetic acid (EDTA), 0.10mg of thiamine, 0.04mg of glycine, 0.01mg of niacin and 0.01mg of pyridoxine. Compared with the original culture medium, the nano-zinc oxide-based culture medium has the advantage that the growth speed of the piriformospora indica is obviously faster; the fresh weight of thallus cultured by using the medium is respectively increased by 36.5% and 55.8% compared with the fresh weights of control groups; the dry weight of the thallus cultured by using the medium is respectively increased by 19% and 26.2% compared with the dry weights of control groups; and the bacterial colony is more compact and smoother than the original bacterial colony. The method provided by the invention is simple, uses easily available materials and is low in cost and good in economic benefit.