Method for improving yield of liquid fermentation products of Piriformospora indica

A technology of Indian piriformis and liquid fermentation, which is applied in the field of bioengineering to achieve the effect of promoting production, simple preparation method and promoting effect

Inactive Publication Date: 2018-03-27
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other uses of cold shock protein have not been reported

Method used

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  • Method for improving yield of liquid fermentation products of Piriformospora indica
  • Method for improving yield of liquid fermentation products of Piriformospora indica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Material preparation

[0041] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0042] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activating culture at 22° C. for 15 days to obtain the activated Pyromorpha indica species.

[0043] Liquid seed medium: glucose 20g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0044] The solid medium is composed of the following components in mass percentage: 0.3% corn flour, 0.6% beef extract, K 2 HPO 4 0.1%, MgSO 4 0.15% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0045] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min....

Embodiment 2

[0052] 1. Material preparation

[0053] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0054] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activating and culturing at 28° C. for 4 days to obtain the activated Pyromorpha indica species.

[0055] Liquid seed medium: glucose 5g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0056] The solid medium is composed of the following components in mass percentage: corn flour 0.9%, beef extract 0.1%, K 2 HPO 4 0.25%, MgSO 4 0.05% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0057] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 2...

Embodiment 3

[0064] 1. Material preparation

[0065] PDA plate medium: potato 200g, glucose 20g and agar 18g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 30min.

[0066] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activation and culture at 26° C. for 6 days to obtain the activated Pyromorpha indica species.

[0067] Liquid seed medium: glucose 10g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.

[0068] The solid medium is composed of the following components in mass percentage: 0.6% corn flour, 0.3% beef extract, K 2 HPO 4 0.2%, MgSO 4 0.1% and the rest of water, with natural pH, sterilized at 121°C for 20min.

[0069] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min....

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Abstract

The invention discloses a method for improving yield of liquid fermentation products of Piriformospora indica. The method comprises the following steps: a Piriformospora indica liquid seed solution isinoculated to a solid culture medium with added exogenous substances for culture, and after vacuum freeze drying and pulverizing are carried out for cultivated products, an exogenous substance solidfermentation culture is obtained; exogenous substances are selected from one or two kinds of fresh rehmannia roots and gardenia fruits; the Piriformospora indica liquid seed solution is inoculated toa liquid fermentation culture medium with the added exogenous substance solid fermentation culture, cultivation is carried out for a period of time, cold shock proteins are added, cultivation is continued for a period of time, and after fermentation is terminated, the fermentation products of Piriformospora indica are obtained. The method can be used for effectively promoting generation of Piriformospora indica metabolites.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for increasing the yield of liquid fermentation products of Pyromorpha indica. Background technique [0002] Piriformospora indica (Piriformospora indica) was obtained when Ajit Verma et al. isolated spores from the rhizosphere soil of shrubs in the Thar Desert in northwest India in 1998. It is a mycorrhizal fungus capable of pure culture. It belongs to Basidiomycota, Hymenomycetes, and has typical pyriform chlamydospores. [0003] Studies have shown that not only the mycelium and spores of Pyromorpha indica can promote the growth of plants, but also its metabolites can promote the growth of plants, making it have a wide application prospect. [0004] The growth rate of Pyriformis indica is relatively slow on the traditional Kafer medium. In order to promote the liquid culture output of Pyromorpha indica and improve the effect of metabolites, inventing a method...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N1/14C12R1/645
CPCC12N1/14C12P1/02C12N1/145C12R2001/645
Inventor 刘慧春田丹青李明江金亮朱开元刘建新俞信英王炜勇潘晓韵葛亚英
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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