Method for improving yield of liquid fermentation products of Piriformospora indica
A technology of Indian piriformis and liquid fermentation, which is applied in the field of bioengineering to achieve the effect of promoting production, simple preparation method and promoting effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] 1. Material preparation
[0041] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.
[0042] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activating culture at 22° C. for 15 days to obtain the activated Pyromorpha indica species.
[0043] Liquid seed medium: glucose 20g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.
[0044] The solid medium is composed of the following components in mass percentage: 0.3% corn flour, 0.6% beef extract, K 2 HPO 4 0.1%, MgSO 4 0.15% and the rest of water, with natural pH, sterilized at 121°C for 20min.
[0045] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min....
Embodiment 2
[0052] 1. Material preparation
[0053] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.
[0054] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activating and culturing at 28° C. for 4 days to obtain the activated Pyromorpha indica species.
[0055] Liquid seed medium: glucose 5g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.
[0056] The solid medium is composed of the following components in mass percentage: corn flour 0.9%, beef extract 0.1%, K 2 HPO 4 0.25%, MgSO 4 0.05% and the rest of water, with natural pH, sterilized at 121°C for 20min.
[0057] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 2...
Embodiment 3
[0064] 1. Material preparation
[0065] PDA plate medium: potato 200g, glucose 20g and agar 18g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 30min.
[0066] Inoculate the preserved Pyromorpha indica species on the PDA plate medium for activation and culture at 26° C. for 6 days to obtain the activated Pyromorpha indica species.
[0067] Liquid seed medium: glucose 10g, yeast powder 4g, peptone 3g, KH 2 PO 4 1g and MgSO 4 0.5g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min.
[0068] The solid medium is composed of the following components in mass percentage: 0.6% corn flour, 0.3% beef extract, K 2 HPO 4 0.2%, MgSO 4 0.1% and the rest of water, with natural pH, sterilized at 121°C for 20min.
[0069] The composition of the liquid fermentation medium is: glucose 15g, corn flour 4g, beef extract 3g, KH 2 PO 4 1.0g and MgSO 4 0.55g, dilute to 1000mL with water, keep the pH natural, and sterilize at 121°C for 20min....
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com