Method for direct seeding and seedling raising of pleione bulbocodioides by using symbiotic bacteria

Abstract

The invention provides a method for direct sowing and seedling raising of pleione bulbocodioides by utilizing symbiotic bacteria, which comprises the following steps: mixing piriformospora indica into a culture medium, sowing pleione bulbocodioides seeds, and performing symbiotic culture to obtain symbiotic pleione bulbocodioides seedlings. The method is simple and convenient to operate, the symbiotic bacteria and pleione bulbocodioides seeds are directly sown for seedling raising for the first time, after 10-15 days, most of the seeds appear green, after 30 days, a large number of protocorms appear native meristem, after 45 days, the protocorms grow first leaves, after 2 months, seedlings form 2-3 leaves and grow roots, and the seedlings can be transplanted to a nursery garden.

Description

Technical field [0001] The present invention belongs to the field of Lanke plant cultivation technology, which involves a method that uses symbiotic bacteria for livelihood seedlings. Background technique [0002] Pleione Bulbocodioides is a unique flower resource in my country. It is listed as a national second -level protection plant and is mostly distributed in the southwest mountain forest. In the wild, this type can be attached to the branches of a variety of trees, with gorgeous colors, long flowering period, and high ornamental value. The species of this species is included in the pills of the pills as "Yami Mushrooms" or "Ice Hockey", which has the effects of treating bloated and poison, sputum nucleus, lymph node tuberculosis and snake bugs. [0003] Lanke plants can reproduce through natural division and organizational training, but the natural division of breeding coefficients is low, and the organizational training technology is not yet mature. Based on the exploratio...

AI Technical Summary

Problems solved by technology

Based on the exploration of the nutritional conditions required by different species, many orchids have been able to obtain seedlings through aseptic sowing, but the production cost of sterile germination seedlings is high and the steps are cumbersome, such as seed germination, protocorm differentiation, and seedling subculture. Different media are usually required for the stage of rooting and strong seedlings, and the aseptic seedlings grow slowly after being released from the bottle and are susceptible to disease.

The artificial cultivation of garlic orchids is still in its infancy. It mainly relies on aseptic seed germination technology to obtain seedlings. It needs to be carried out under laboratory conditions-on the culture medium in sterile glass bottles. The operation is complicated and the conditions are harsh and the cost is high.

Although the symbiotic germination technology can improve the germination rate of seeds, the differentiation speed of protocorms and the survival rate of seedlings, there are only a handful of symbiotic strains reported with Orchidaceae species, and some symbiotic cultures are limited to culture bottles.

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