34 results about "Xenorhabdus sp." patented technology

The invention discloses Brevundimonas olei dahliae olei and application thereof. The Brevundimonas olei dahliae olei is named as Brevundimonas olei dahliae olei, and the preservation number of the Brevundimonas olei dahliae olei is CCTCC NO: M 2020392. The strain is determined to be Brevundimonas olei dahliae olei through morphological identification and diversity sequencing analysis, and the influence of B.olei fermentation liquor on growth of the Brevundimonas oleei and microsclerosis is explored through a co-culture experiment. Through scanning electron microscope observation, as the culture time and the concentration of the fermentation liquor increase, formation of conidia of the Brevundimonas oleei is reduced, the hypha morphology is expanded, broken and deformed, the microsclerosisis gradually reduced and disappears, and it is proved through the research that the symbiotic B.olei has good bacteriostatic activity on the Brevundimonas olei.

The invention provides a single bud propagation method of Phalaenopsis phalaenopsis, comprising the following steps: S1, taking a pedicel stem section that grows robustly in spring and full with axillary buds as explants and carries out disinfection treatment; S2, cutting the explants with axillary buds into pieces Grow 1.5cm and receive it into the bud induction medium for axillary bud induction; S3, when the axillary bud grows to 2cm in step S2, cut the sprouts and transfer them to the proliferation medium for proliferation culture; S4, when the proliferation culture sprouts > 2cm , when it grows to 3-4 leaves, remove weak seedlings and ineffective seedlings less than 2 cm, select seedlings > 2 cm to carry out rooting culture of strong seedlings; S5, move the culture bottle seedlings to 25-30 ℃ temperature conditions to strengthen seedlings for 10- After 15 days, the rooted seedlings can be taken out of the culture bottle, the residual medium attached to the roots is washed with running tap water and dried until no water drips, and then transplanted into the pre-prepared mixed matrix and pressed. If it is planted, it should be watered for the first time after planting, and then every 4-6 days after that. The method has the advantages of short reproductive cycle, strong seedlings, strong adaptability and good quality.

The invention discloses a bacterium strain (X-7 system) to culture bait nematolog and nematolog culturing method, which comprises the following steps: culturing Xenorhabdus sp. X-7 CCTCC M 206090 on the culture medium with peptone water under 25-28 deg. c to obtain the bacterial liquid; adding nematolog culture medium; switching in Panagrellus redivivus; culturing under 25-28 deg. c for 6-15h; obtaining the product as bait in the aquatic product culturing industry.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.