34 results about "Xenorhabdus sp." patented technology

The invention discloses an isolated culture method for nilaparvata lugens in-vivo yeast-like symbiotic bacteria and a special culture medium, and belongs to the technical field of microculture. The special culture medium comprises 31 nutrient substances needed by nilaparvata lugens in-vivo yeast-like symbiotic bacteria, and the culture method comprises the following steps of: feeding adult nilaparvata lugen; treating ovaries of nilaparvata lugens; preparing culture solution, performing isolated culture and the like. By employing the culture method, two yeast-like strains are isolated from the nilaparvata lugens in vivo so as to provide basis for further researching genetic background of the yeast-like symbiotic bacteria and the interavailable nutrient relation between the yeast-like symbiotic bacteria and host nilaparvata lugens, and modifying the symbiotic bacteria to control the harm of the nilaparvata lugens. Meanwhile, the special culture medium is reasonable in components and provides necessary nutrition basis for isolated subculture of the nilaparvata lugens in-vivo yeast-like symbiotic bacteria. The method also provides technical support for isolated culture of other homoptera insect in-vivo yeast-like symbiotic bacteria.

The invention relates to the technical field of biological control, in particular to application of nativo to control over filial generations of brown planthopper. Native water dispersible granule (with 75 % mass percent of active ingredients including 25 % mass percent of trifloxystrobin and 50% mass percent of tebuconazole) is utilized by the invention for control over filial generations of brown planthopper.The most salient feature of the invention is described in that nativo, as bactericide, helps to prevent of inhibit yeast-like endosymbiote from entering ovarian tissues from hemolymph of female adults of brown planthopper such that a genital system of brown planthopper is affected and the egg laying amount is reduced; and the hatching rate is greatly decreased because produced eggs lack symbiotic bacteria so that generation of the filial generations of brown planthopper is effectively controlled.

The invention provides a culture medium for Amylostereum areolatum and a culture method thereof. The culture medium comprises healthy pine wood flour, malt extract and yeast extract. The culture medium for Amylostereum areolatum provided by the invention has the advantages that the components and the proportion of the culture medium are scientific and reasonable, the growth speed of the cultured Amylostereum areolatum is higher, part of the Amylostereum areolatum can grow sporocarp, the Amylostereum areolatum is not easy to be polluted, and a foundation is laid for further research of symbiotic bacteria of pine bees and even prevention and control of the pine bees.

The invention discloses an application of ascaroside in the preparation of a preparation for promoting the development recovery of nematodes in a Steinernema carpocapsae All infection period. According to the application disclosed by the invention, compared with a symbiotic bacteria liquid supernatant as a control, the ascaroside can be used for remarkably promoting the development recovery of the nematodes in the S.carpocapsae All infection period. Therefore, in the mass culture process of the nematodes, the ascaroside can be used for inducing the nematodes in the S.carpocapsa All infection period to recover development, and a core technology is provided for commercialized production of the S.carpocapsa All nematodes with cost advantages.

The invention discloses a bletilla striata direct seeding and seedling raising method using symbiotic bacteria. The method specifically comprises the following steps: S1, acquiring of the symbiotic bacteria of bletilla striata: culturing a bletilla striata explant to obtain a strain of the symbiotic bacteria of bletilla striata for later use; S2, direct sowing and seedling raising of bletilla striata: mixing pretreated bletilla striata seeds and the symbiotic bacteria of bletilla striata in the step S1, and then sowing the seeds in a seedbed; S3, sowing later-period management: after the sown seeds germinate and emerge, carrying out fertilization and seedling hardening treatment, and then transplanting. The symbiotic bacteria of bletilla striata and bletilla striata seeds are uniformly mixed, direct seeding is performed, then seedling hardening treatment is performed, due to the fact that the symbiotic bacteria of bletilla striata are added in the germination process of the bletilla striata seeds, the direct seeding germination rate of the bletilla striata seeds is as high as 86%, a Huabao leaf fertilizer is sprayed in the later-period management process, and the seedling growth speed is high. Compared with a tissue culture seedling raising method, the method has the advantages that the production cost and labor consumption are greatly reduced, the cultured bletilla striata seedlings are vigorous and uniform in growth vigor, and the transplanting survival rate reaches up to 99%.