Extraction and purification method of anoectochilus roxburghii symbiotic bacteria

Abstract

The invention discloses an extraction and purification method of anoectochilus roxburghii symbiotic bacteria. The extraction and purification method comprises the following steps: S1, removing mixed bacteria of anoectochilus roxburghii; S2, separating mother strains of the symbiotic bacteria; S3, purifying and culturing the symbiotic bacteria; and S4, expanding culture of the symbiotic bacteria. The method is used for producing the symbiotic bacteria in the anoectochilus roxburghii tissue culture and planting industry, the growth period of the anoectochilus roxburghii can be obviously shortened by adding the symbiotic bacteria into an anoectochilus roxburghii tissue culture sterile culture medium or an anoectochilus roxburghii planting culture medium, leaves of plants are more stretched, golden lines of the leaves are more obvious, stem nodes are shortened, and single plant quality is improved by 20% in average; in addition, the pollution rate of the culture medium added with the symbiotic bacteria is reduced by 30%, and the requirement of the anoectochilus roxburghii tissue culture industry for symbiotic bacteria additive products can be met; meanwhile, the preparation process issimple, operation is easy, the symbiotic bacteria can be added for co-culture in tissue culture seedlings of anoectochilus roxburghii produced in factories, the quality and yield of anoectochilus roxburghii seedlings are improved, and the survival rate and environmental adaptability of later underground cultivation of the anoectochilus roxburghii are facilitated.

Description

technical field [0001] The invention belongs to the field of cultivating plant symbiotic fungi, in particular to a method for extracting and purifying endophytic fungus from Clematis aureus. Background technique [0002] Clematis clematis is widely used among the people, and it is known as "the king of medicine", "golden grass", "miracle medicine", "black ginseng" and other laudatory titles. According to the determination of relevant departments, it is found that the amino acid composition, ingredients, content and anti-aging active trace element content of clematis are higher than those of domestic and wild American ginseng. It has been used as folk herbal medicine for hundreds of years. With the improvement of people's living standards, the health care industry is also booming. More and more health-care plant products have given people more choices. With the increase in demand, more and more health-care plant products It has been widely developed, and the technical devel...

AI Technical Summary

Problems solved by technology

With the improvement of people's living standards, the health care industry is also booming. More and more health-care plant products have given people more choices. With the increase in demand, more and more health-care plant products It has been widely developed, and the technical development in the field of plant tissue culture can meet the market's supply of health-care plant raw materials. However, due to the particularity of the environment required for growth, the production cost of Clematis clematis has been high in the tissue culture industry, and the growth The cycle is too long, the yield per plant is not high, and many factors have pushed up the cost of clematis tissue culture seedlings, so that the industry has developed for more than ten years, and many enterprises and funds have invested in clematis tissue culture production, but not There is no cost advantage due to the aggregation effect, which reduces the terminal price

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