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Application of ascaroside in preparation of preparation for development recovery of nematodes in H.bacteriophora H06 infection stage

A technology of infection period and ascaroside, which is applied in the field of biological control, can solve the problems of low recovery rate and low yield of nematodes in the infection period, and achieve a good recovery rate effect

Pending Publication Date: 2021-12-03
YUXI TABACOO COMPANY OF YUNNAN PROVINCE +1
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Problems solved by technology

If the recovery rate is low, the number of first-generation adults is small, and only a small part of the symbiotic bacteria are eaten, and the offspring of the first-generation adults will not be able to form larvae at the infectious stage due to sufficient nutrition, resulting in low yield of nematodes at the infectious stage

Method used

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  • Application of ascaroside in preparation of preparation for development recovery of nematodes in H.bacteriophora H06 infection stage
  • Application of ascaroside in preparation of preparation for development recovery of nematodes in H.bacteriophora H06 infection stage
  • Application of ascaroside in preparation of preparation for development recovery of nematodes in H.bacteriophora H06 infection stage

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Embodiment Construction

[0015] The following examples are to further illustrate the present invention, rather than limit the present invention.

[0016] 1. Experimental materials

[0017] 1. Ascarosides include 11 kinds of ascarosides, the code names are ascr#1, ascr#2, ascr#3, ascr#5, ascr#6, ascr#7, ascr#8, ascr#9, ascr#10, ascr# #11, ascr#12, their structural formulas are listed as follows:

[0018]

[0019] Dissolve the above 11 kinds of ascarosides in sterile PBS to 0.5mM respectively, then dilute to a certain concentration, and add a certain amount to the symbiotic bacterial supernatant or medium, so that it can be dissolved in the symbiotic bacterial supernatant or medium The concentrations in are 0.04μM, 0.04nM, 0.04pM, respectively.

[0020] 2. H.bacteriophora H06 cultured by symbiotic bacteria: After washing the nematodes cultured on conventional solid medium, add streptomycin sulfate solution (concentration: 100ppm) to treat overnight at 25°C, and wash three times with sterile PBS buf...

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Abstract

The invention discloses application of ascaroside in the preparation of a preparation for promoting the development recovery of nematodes in a Heterorhabditis bacteriophora H06 infection stage. According to the application disclosed by the invention, compared with a contrast symbiotic bacterium liquid supernatant, the ascaroside can be used for remarkably promoting the development recovery of the nematodes in the H.bacteriophora H06 infection stage, and the ascaroside can be used for inducing the development recovery of the nematodes in the H.indica LN2 infection stage. Therefore, in the mass culture process of the nematodes, the ascaroside is added into a culture medium added with the H.bacteriophora H06 infection stage nematodes containing corresponding symbiotic bacteria, and the nematodes in the infection stage are induced to recover development and growth. The core technology is provided for commercially producing the H.indica LN2 nematodes with a cost advantage.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to the application of ascarosides in the preparation of Heterorhabditisbacteriophora H06 infection period nematode recovery development preparations. Background technique [0002] Entomopathogenic Steinernema and Heterorhabditis nematodes (Entomopathogenic nematode) are new and highly effective biological insecticides in the world. This kind of nematode has strong insecticidal ability, wide insecticidal spectrum, can actively search for hosts, is safe for humans, animals and the environment, is easy to cultivate in large quantities, and can be mixed with a variety of chemical pesticides, and has special effects on borers and underground pests. Symbiotic bacteria of the genus Xenorhabdus or Photorhabdus carried in the gut of entomopathogenic nematodes play an important role in restoring development during infection, killing insects, and transforming nutrients for nematod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王杰谷星慧屈玲李江舟曹莉韩日畴黄智华崔永和杨海林杨晓俊
Owner YUXI TABACOO COMPANY OF YUNNAN PROVINCE
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