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Culture medium for Amylostereum areolatum and culture method thereof

A cultivation method and technology of bacilli, which are applied in the field of microorganisms, can solve the problems of slow growth of symbiotic bacteria, lack of fruit bodies, and general effects, etc., and achieve fast growth, not easy to pollute, and scientific and reasonable composition and ratio Effect

Pending Publication Date: 2021-10-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The existing research results show that the effect of using potato dextrose agar medium (PDA) to cultivate pine wasp symbiotic bacteria is average, and the main performance is that the symbiotic bacteria grow slowly and cannot grow fruiting bodies, etc.

Method used

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  • Culture medium for Amylostereum areolatum and culture method thereof
  • Culture medium for Amylostereum areolatum and culture method thereof
  • Culture medium for Amylostereum areolatum and culture method thereof

Examples

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Embodiment 1

[0033] This embodiment provides a culture medium for Adenobacter reticulum and cultures for Adenobacter reticulum.

[0034] Each liter of PPMYA contains: 175.0 g of healthy pine wood powder, 15.0 g of agar, 3.0 g of malt extract, 0.3 g of yeast extract, and the balance is water.

[0035] The culture medium was prepared as follows:

[0036] Split pine wood and pine wood without pest damage and obvious fungal infection into small pieces of 1.5×0.7×0.3cm, dry at 90°C and grind them into wood powder, then sieve with a 60-mesh sieve, and Sterilize at 117°C for 20 minutes to obtain healthy sylvestris sylvestris sylvestris pine wood powder and healthy oil pine wood powder respectively.

[0037] After 24 hours, weigh 15.0g of agar and 175.0g of healthy sylvestris sylvestris pine wood powder, and evenly distribute them in 5 Erlenmeyer flasks. Use a beaker to measure 3.0g of malt extract and 0.3g of yeast extract, add 50ml of distilled water to each, heat with an alcohol lamp until th...

Embodiment 2

[0044] The difference between the culture medium for the sclerotium sclerotium provided in this example and that of Example 1 is that the healthy sylvestris sylvestris sylvestris pine wood powder is replaced with an equal amount of healthy pine wood powder.

[0045] The method for cultivating the adenobacteria reticulum provided in this embodiment is the same as that in embodiment 1.

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Abstract

The invention provides a culture medium for Amylostereum areolatum and a culture method thereof. The culture medium comprises healthy pine wood flour, malt extract and yeast extract. The culture medium for Amylostereum areolatum provided by the invention has the advantages that the components and the proportion of the culture medium are scientific and reasonable, the growth speed of the cultured Amylostereum areolatum is higher, part of the Amylostereum areolatum can grow sporocarp, the Amylostereum areolatum is not easy to be polluted, and a foundation is laid for further research of symbiotic bacteria of pine bees and even prevention and control of the pine bees.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a culture medium and a culture method for Adenoides reticulum. Background technique [0002] The pine wasp Sirex noctilio Fabricius belongs to the genus Sirex of the order Hymenoptera (Hymenoptera), the family Siricidae, and is a devastating international forestry quarantine pest. In my country, the known host tree species of the insect is Pinus sylvestris sylvestris var. mongolica Litv.). In addition to directly boring trees, the pine wasp can also inject the toxin secreted by itself and the symbiotic fungi in the host tree through the behavior of laying eggs. The three harmful factors of "insect-toxin-bacteria" coordinate with each other to harm the host tree together. , resulting in weakening or even death of the host tree. [0003] The symbiotic bacteria of the pine wasp is mainly a white-rot fungus, the species name is Amylostereumareolatum, which belongs to Basidiomycota ...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40A01G18/00
CPCA01G18/20A01G18/40A01G18/00
Inventor 王丽霞任利利骆有庆王明詹钟易
Owner BEIJING FORESTRY UNIVERSITY
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