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Application of ascaroside in preparation of preparation for promoting development recovery of nematodes in Steinernema carpocapsae All infection period

A technology of infection period and ascaroside, applied in the field of biological control, to achieve the effect of good recovery rate

Pending Publication Date: 2021-11-02
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has not been reported whether these ascarosides can induce nematodes to recover during entomopathogenic infection

Method used

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  • Application of ascaroside in preparation of preparation for promoting development recovery of nematodes in Steinernema carpocapsae All infection period
  • Application of ascaroside in preparation of preparation for promoting development recovery of nematodes in Steinernema carpocapsae All infection period
  • Application of ascaroside in preparation of preparation for promoting development recovery of nematodes in Steinernema carpocapsae All infection period

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Experimental program
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Effect test

Embodiment 1

[0017] 1. Experimental materials

[0018] 1. Ascarosides include 11 artificially synthesized ascarosides, whose code names are ascr#1, ascr#2, ascr#3, ascr#5, ascr#6, ascr#7, ascr#8, ascr#9, ascr# 10. Ascr#11, ascr#12, their structural formulas are listed as follows:

[0019]

[0020] Dissolve the 11 kinds of ascarosides in sterile PBS to 0.5mM, and then dilute to a certain concentration and add a certain amount to the supernatant or culture medium of symbiotic bacteria to make the concentration in the supernatant or culture medium of symbiotic bacteria They are 0.04μM, 0.04nM, 0.04pM, respectively.

[0021] 2. S.carpocapsae All cultured by symbiotic bacteria: After cleaning the S.carpocapsae All nematodes cultured on conventional solid medium, add streptomycin sulfate solution (concentration: 100ppm) to treat overnight at 25°C, and use sterile PBS After washing with the buffer solution three times, the precipitate was prepared with sterile PBS to a concentration (5000 IJ...

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Abstract

The invention discloses an application of ascaroside in the preparation of a preparation for promoting the development recovery of nematodes in a Steinernema carpocapsae All infection period. According to the application disclosed by the invention, compared with a symbiotic bacteria liquid supernatant as a control, the ascaroside can be used for remarkably promoting the development recovery of the nematodes in the S.carpocapsae All infection period. Therefore, in the mass culture process of the nematodes, the ascaroside can be used for inducing the nematodes in the S.carpocapsa All infection period to recover development, and a core technology is provided for commercialized production of the S.carpocapsa All nematodes with cost advantages.

Description

Technical field: [0001] The invention belongs to the field of biological control, and in particular relates to the application of ascarosides in the preparation of preparations for promoting the recovery and development of nematodes in the infection stage of S. carpocapsae All. Background technique: [0002] The entomopathogen Steinernematidae and Heterorhabditidae nematodes actively search for host insects in the 3rd instar infection stage. They are safe to non-target organisms and the environment, and can be produced on a large scale. They are natural enemies with application potential and have been widely used. It is used to control pests in fields such as agriculture, forestry, pasture, flowers and sanitation. Entomopathogenic nematodes and the symbiotic bacteria carried in their bodies work together to kill host insects. They enter the insect body with the host food or from the insect's natural opening (such as anus, stigma) and intersegment membrane in the stage of in...

Claims

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Application Information

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IPC IPC(8): A23K20/121A23K50/00A01K67/033A01N63/12A01P7/04
CPCA23K20/121A23K50/00A01K67/033A01N63/12
Inventor 曹莉王杰屈玲戴康孔祥鑫徐成体李玉玲金永玲韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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