Bletilla striata direct seeding and seedling raising method using symbiotic bacteria

Abstract

The invention discloses a bletilla striata direct seeding and seedling raising method using symbiotic bacteria. The method specifically comprises the following steps: S1, acquiring of the symbiotic bacteria of bletilla striata: culturing a bletilla striata explant to obtain a strain of the symbiotic bacteria of bletilla striata for later use; S2, direct sowing and seedling raising of bletilla striata: mixing pretreated bletilla striata seeds and the symbiotic bacteria of bletilla striata in the step S1, and then sowing the seeds in a seedbed; S3, sowing later-period management: after the sown seeds germinate and emerge, carrying out fertilization and seedling hardening treatment, and then transplanting. The symbiotic bacteria of bletilla striata and bletilla striata seeds are uniformly mixed, direct seeding is performed, then seedling hardening treatment is performed, due to the fact that the symbiotic bacteria of bletilla striata are added in the germination process of the bletilla striata seeds, the direct seeding germination rate of the bletilla striata seeds is as high as 86%, a Huabao leaf fertilizer is sprayed in the later-period management process, and the seedling growth speed is high. Compared with a tissue culture seedling raising method, the method has the advantages that the production cost and labor consumption are greatly reduced, the cultured bletilla striata seedlings are vigorous and uniform in growth vigor, and the transplanting survival rate reaches up to 99%.

Description

technical field [0001] The invention belongs to the technical field of raising seedlings of traditional Chinese medicinal materials, and in particular relates to a method for direct-seeding seedling raising of bletilla striata using symbiotic bacteria. Background technique [0002] Bletilla striata [Bletilla striata (Thunb.ex A.Murray) Rchb.f.], also known as Bletilla striata, is a perennial herb of the genus Bletilla striata in the family Orchidaceae. Bletilla striata is a famous and precious Chinese herbal medicine in my country, which has the effects of astringent and hemostasis, detumescence and muscle regeneration. Chemical analysis shows that Bletilla striata tubers contain bibenzyls, polysaccharides, dihydrophenanthrenes, polyphenols, flavonoids and other organic compounds; modern pharmacological studies have shown that these organic compounds have anti-cancer, anti-oxidation, therapeutic Various biological activities such as cardiovascular disease. Bletilla striata...

AI Technical Summary

Problems solved by technology

However, the production cost of bletilla striata group is high, labor consumption is large, and the survival rate of transplanting is relatively low, which cannot meet the needs of the medicinal material market for seedlings