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A kind of parasitic small rod nematode preparation and its preparation method and application

A small rod nematode, parasitic technology, applied in the directions of biochemical equipment and methods, botanical equipment and methods, applications, etc., can solve the problems of inability to kill Spodoptera frugiperda efficiently and quickly, and low biocontrol quick effect. , to achieve the effect of easy popularization and application, simple operation and simple preparation method

Active Publication Date: 2021-08-17
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for Spodoptera frugiperda, the use of insect parasitic nematodes has the problem of low biocontrol efficiency, and cannot kill Spodoptera frugiperda efficiently and quickly.

Method used

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  • A kind of parasitic small rod nematode preparation and its preparation method and application
  • A kind of parasitic small rod nematode preparation and its preparation method and application
  • A kind of parasitic small rod nematode preparation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example provides the ingredient content table and preparation method of the parasitic C. nematode preparation of the present invention, see Table 1 for details.

[0036] Table 1

[0037]

[0038] Adopt the raw material in table 1 to prepare the preparation method of parasitic small rod nematode preparation of the present invention as follows:

[0039] Step 1. Expansion and cultivation of parasitic small rod nematodes:

[0040] The parasitic rod nematodes are infected and multiplied by the 5-6 instar larvae of Spodoptera frugiperda; the reproduced parasitic rod nematode third instar larvae (IJs) are collected in distilled water by the white trap method and stored in a refrigerator at 4°C Use after 2 weeks;

[0041] Step 2. Take each raw material component in proportion:

[0042] Concentrate the parasitic C. nematode suspension collected in step 1 by the static sedimentation method, add dsRNA samples, add distilled water to make up to 1000 mL, mix at room tempe...

Embodiment 2

[0048] This example provides a composition content table of the parasitic C. nematode preparation of the present invention, see Table 2 for details.

[0049] Table 2

[0050]

[0051] Adopt the raw material in table 2 to prepare the preparation method of parasitic small rod nematode preparation of the present invention as follows:

[0052] Step 1. Expansion and cultivation of parasitic small rod nematodes:

[0053] The parasitic small rod nematodes are infected and multiplied by the 5-6 instar larvae of Spodoptera frugiperda; the third instar larvae (IJs) of the parasitic small rod nematodes are collected in distilled water by the white trap method and stored in a refrigerator at 4°C for 2 weeks after use;

[0054] Step 2. Weigh the pest attractant matrix, humectant, and symbiotic bacteria nutrients into the same container in proportion, add distilled water to make up to 900mL, mix well and put it into a triangular flask, cover it with a sealing film and put it in an auto...

Embodiment 3

[0057] This example provides the application of the preparation of the present invention to control Spodoptera frugiperda.

[0058] The specific application is:

[0059] 1. Indoor bioactivity assay:

[0060] Make the area about 10cm 2 The surface of corn leaves was sterilized with 75% alcohol, dried naturally, and placed in a petri dish (diameter 9 cm) with 2 layers of filter paper for routine culture. Ten 5th instar larvae of Spodoptera frugiperda were inserted into each dish. Dilute different preparations of Bacillus nematodes 10 times and add them on the filter paper of petri dishes, add 0.25ml preparations of Bacillus nematodes to each dish (2.5ml / dish of diluted preparations), repeat each treatment 3 times, and treat with clear water as blank control . 8h, 16h, 24h, and 32h after inoculation with Spodoptera frugiperda larvae, observe and count the number of dead insects in each treatment.

[0061] Survival rate=(survival number / test worm number)×100%; Corrected death...

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Abstract

The invention discloses a parasitic small rod nematode preparation and its preparation method and application, belonging to the technical field of biological pesticides, solving the problem of low quick-acting biocontrol in the prior art, and unable to efficiently and quickly kill Spodoptera frugiperda The problem. The parasitic small rod nematode preparation of the present invention comprises: parasitic small rod nematodes, an RNA segment that interferes with the expression of the polyphenol oxidase PO gene of Spodoptera frugiperda; also includes a pest attractant matrix, a nematode moisturizing agent, and nematode symbiotic bacteria nutrients . The invention creatively starts from various aspects such as attracting, infecting, saprophytic, and adding dsRNA to suppress the immune response of pests, and works together to effectively improve the killing effect of nematodes on Spodoptera frugiperda.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a parasitic small rod nematode preparation, a preparation method and an application. Background technique [0002] Spodoptera frugiperda (J.E.Smith), native to tropical and subtropical regions of America, is a moth-like agricultural pest belonging to the family Noctuidae. Spodoptera frugiperda larvae can eat a large number of gramineous grains such as rice, sugarcane, and corn, as well as a variety of crops such as Asteraceae and Cruciferae. Their development speed will increase with the increase of temperature. It can reproduce several generations a year, and a female moth can lay more than 1,000 eggs. After invading West Africa in January 2016, it quickly spread to 44 sub-Saharan countries. Spodoptera frugiperda invaded India in May 2018, moved from Myanmar to China on December 11, 2018, and had spread to 26 provinces (autonomous regions, municipalities) in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/12A01N63/60A01P7/04C12N15/113
CPCA01N63/12A01N63/60C12N15/113C12N2310/14
Inventor 李星月张鸿杨武云符慧娟陈鹏李其勇易军陆文壹
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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