Symbiotic bacterium composition, preparation method thereof and microalgae culture method

A technology of microalgae culture solution and symbiotic bacteria, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as industrial application impact, residue, and biological safety concerns, and achieve the purpose of suppressing the risk of pests and diseases, The effect of reducing hazards and increasing production capacity

Pending Publication Date: 2021-08-20
HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially harmful bacteria such as molds and pathogenic bacteria, not only competitively absorb the nutrients in the culture solution, inhibit the growth of algae, destroy the integrity of algae cells, but also remain in the algae solution, affecting downstream industrial applications
[0013] At present, the commonly used antibacterial method in the industry is to add various antibiotics to inhibit the growth of bacteria, but the introduction of antibiotics will bring about biological safety concerns, and will also inhibit the normal growth of algae

Method used

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  • Symbiotic bacterium composition, preparation method thereof and microalgae culture method
  • Symbiotic bacterium composition, preparation method thereof and microalgae culture method
  • Symbiotic bacterium composition, preparation method thereof and microalgae culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0073] Example 1 Probiotic culture, preparation of symbiotic bacteria liquid and co-cultivation mode

[0074] (1) Clostridium butyricum culture

[0075] 1) Strain: Clostridium butyricum marine

[0076] 2) Medium formula (1L):

[0077] Table 1

[0078] Material name Dosage(g / L) Peptone 11 Beef Dip Powder 10 Yeast Dip Powder 4 Dipotassium phosphate 5 sodium acetate 8 Triammonium citrate 2 magnesium sulfate 0.2 Manganese sulfate 0.05 Tween 80 1 glucose 25 calcium carbonate 5 Sodium chloride 20 purified water 1L

[0079] 3) The above-mentioned culture medium was mixed in an Erlenmeyer shaker flask, and sterilized at 121° C. for 25 minutes.

[0080] 4) Resuscitate 1 ml of frozen Clostridium butyricum marine strains in a water bath, then insert them into the sterilized culture medium with an inoculum size of 10%, and put them into a shaker for cultivation.

[0081] 5) Culture conditions:...

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Abstract

The invention relates to the technical field of microalgae culture, in particular to a symbiotic bacterium composition, a preparation method thereof and a microalgae culture method. The symbiotic bacterium composition consists of marine clostridium butyricum, marine rhodotorula benthica and marine purple non-sulfur bacteria. Through the symbiotic effect of the three probiotics including the marine clostridium butyricum, the marine rhodotorula benthica and the marine purple non-sulfur bacteria, the risk of diseases and pests in the seawater chlorella culture process is effectively inhibited, the harm of the diseases and pests is reduced, and the capacity and yield of chlorella factory culture are effectively improved.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a symbiotic bacteria composition, a preparation method thereof and a microalgae cultivation method. Background technique [0002] Seawater chlorella is widely used in the fields of pharmaceutical raw materials, health food and bioenergy because it is rich in protein, lipid, polysaccharide, vitamin, antioxidant and other functional nutrients. At present, seawater chlorella culture technology is mostly concentrated in the photoautotrophic system, which has disadvantages such as low production efficiency, large floor area, and high harvest cost, which seriously restricts the development of seawater chlorella industry. Increasing the biomass of seawater chlorella through artificial large-scale cultivation technology is the key to the development and utilization of seawater chlorella resources. In recent years, seawater chlorella heterotrophic high cell density culture...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/12C12R1/145C12R1/645C12R1/01C12R1/89
CPCC12N1/20C12N1/16C12N1/12
Inventor 黄成潭潘军叶蕾黄敏
Owner HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD
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