Construction method and application of phenanthrene and pyrene combined aerobic degradation symbiotic flora

Abstract

The invention relates to a construction method and application of a phenanthrene and pyrene combined aerobic degradation symbiotic flora, and the phenanthrene and pyrene combined aerobic degradation symbiotic flora uses four strains, namely Pseudomonas.sp,GenBank:GCA_000006765.1, Bacillus.sp,GenBank:AY881640.1, Sphingomonas.sp,GenBank:AB000106.1 and achromobacter.sp,GenBank:GCA_004296055.1. The construction method comprises an amplification culture method of each strain, an enhanced medium formula, culture conditions and operation methods, wherein the phenanthrene and pyrene combined aerobic degradation symbiotic flora is applied to biological extracellular polymers in a reactor device, the reactor device comprises a pipeline and a microbial inoculum ball group, and the biological extracellular polymers prepared from four strains are loaded in the microbial inoculum ball group. The method has the advantages that biodegradation of phenanthrene and pyrene in the chemical wastewater is achieved in an aerobic environment, secondary pollution is avoided in the treatment process, resources can be saved, the ecological environment can be protected, and finally, the purpose of treating degradation-resistant organic matter namely the phenanthrene and the pyrene is achieved.

Description

technical field [0001] The invention relates to the field of biological treatment of chemical wastewater, in particular to a construction method and application of a symbiotic flora for combined aerobic degradation of phenanthrene and pyrene. Background technique [0002] In the treatment process of petrochemical industrial wastewater, persistent organic pollutants are an important class of pollutants, which have attracted international attention because of their four characteristics: environmental persistence, bioaccumulation, long-distance migration ability and high biological toxicity. , has caused a great threat to human health and the environment, and has become an environmental issue that countries around the world attach great importance to. In August 2013, the Fourth Session of the Standing Committee of the Twelfth National People's Congress approved the "Amendment to Add Nine Persistent Organic Pollutants to the Stockholm Convention on Persistent Organic Pollutants"...

AI Technical Summary

Problems solved by technology

According to the research report on the activity and structure of aromatic carcinogens, their products are catalyzed by a certain catalysis in the keto-enol tautomerization process of proteins, which leads to irreversible changes in proteins and ultimately leads to cell canceration.

[0004] At present, the sewage treatment of phenanthrene and pyrene is mainly chemical methods such as Fenton oxidation or ozone oxidation commonly used in chemical sewage. Although this process has a higher rate of chemical degradation and lower cost than conventional aerobic biodegradation, but due to The molecular weight of the intermediate product produced by oxidation is larger than that of the parent, only part of it can be completely converted into non-toxic, low molecular weight products, and most of the rest remain in the environment, causing secondary pollution

At the same time, many toxic intermediates are difficult to be bioavailable after production, and even have greater toxicity. For example, pyrene will be converted into a more toxic intermediate diol pyrene, and cause toxic accumulation

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