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Portable biological crust seed source and preparation method thereof

A biological crust, portable technology, applied in the field of microorganisms, can solve the problems of not easy to carry, environmental pollution, high transportation costs, etc., and achieve the effect of promoting the formation

Pending Publication Date: 2022-06-03
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the phenomenon of soil productivity decline caused by drought and less rain, wind erosion and water erosion in desert areas and sandstorm weather; some sand fixation measures currently used have environmental pollution, high transportation costs, and are not easy to carry. Sand-fixing biological materials in the wild should be environmentally friendly, safe, and easy to carry

Method used

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  • Portable biological crust seed source and preparation method thereof
  • Portable biological crust seed source and preparation method thereof
  • Portable biological crust seed source and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1. Preparation of Portable Biological Crust Provenance

[0055] 1. Activation culture

[0056] Symbiotic bacteria F07020 (it is disclosed in CN201110261963.3, the deposit number in the General Microorganism Center of China Microorganism Culture Collection Management Committee: CGMCC No.3893) is inoculated on the optimized bacteria slant medium, activated for 15 days, and selected The symbiotic bacteria grown on the medium were inoculated into the optimized bacteria liquid medium for activation and culture, and the culture conditions were 22° C. and 150 rpm for 30 days.

[0057] Diplosphaera chodatiiA07020 (which is disclosed in patent CN201110262013.2, the deposit number in the General Microorganism Center of China Microorganism Culture Collection Management Committee: CGMCC No.3892) was inoculated on the optimized algal slant medium, and after activating 15 days , Select the Symbiodinium lithospermum grown on the medium and inoculate it into the optimized alga...

Embodiment 2

[0069] Example 2 Application Verification of Portable Biocrust Provenance

[0070] The bacteria and algae pellets prepared in Example 1 were clamped onto the sand medium with sterile tweezers, and the inoculation density was 9 inoculated at intervals of 90 mm sterile plates. Place the petri dish in an artificial climate box and cultivate at 18°C ​​under 16h light (light intensity 2000lux) / 8h in the dark. After culturing for 1 month, use an environmental scanning electron microscope to observe the morphology and internal structure of the bacteria-algae mixture, such as figure 2 and image 3 shown, from figure 2 and image 3 , it can be seen that the growth state of the bacteria and algae is good, and the microscopic bacteria and algae contact structure is finally produced because the sufficient contact between the symbiotic bacteria and the symbiotic algae can be ensured.

[0071] After culturing in an artificial climate box for 3 months, the plate culture, that is, the ba...

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Abstract

The invention discloses a portable biological crust seed source and a preparation method of the portable biological crust seed source, and the portable biological crust seed source comprises a biological crust seed source small ball prepared from a stone coat symbiotic bacterium (Endocarpon bacillus) F07020, a stone coat symbiotic alga (Diplosphaera chodatii) A07020 and a forming culture medium. The method is favorable for forming a seed source of the lichens which are collected in a state closer to a natural state, can be applied to desertification land control engineering on a large scale, and is favorable for promoting the formation of artificial biological crust.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and provides a method for preparing a portable biological crust seed source, which can simultaneously promote the formation of a symbiotic structure of lichens. Background technique [0002] In desert areas, precipitation is scarce, and cyanobacteria, lichens, moss, bacteria, fungi and other organisms in the soil are attached and entangled on the surface to form a special structure—microbiological crusts. According to the dominant organisms in different succession stages of crusts, it can be divided into cyanobacterial crusts, lichen crusts and moss crusts. With the progress of succession, the stability and anti-interference ability of biological crusts gradually improved, and the biodiversity also showed an upward trend. Lichens are mutualistic symbionts composed of lichen-type fungi and corresponding algae or cyanobacteria, which are widely distributed in nature. They have a long life ...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C09K17/40C12R1/645C12R1/90C09K105/00
CPCC12N11/10C12N11/04C09K17/40C09K2105/00Y02A40/80
Inventor 魏鑫丽程向敏张婷婷杨秋霞王延延魏江春
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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