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Biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and preparation method thereof

A technology of Bacillus and symbiotic culture, applied in the field of biological seed coating agent preparation, can solve the problems of poor stress resistance of bacteria agents, crop disease resistance and stress resistance are not as good as Trichoderma, and can not solve the problems of Trichoderma and Bacillus product convergence , to achieve the effect of stabilizing the spore content

Pending Publication Date: 2021-09-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Single Trichoderma and Bacillus preparations are currently more common in the prevention and control of crop diseases and the promotion of crop growth, but the shortcomings of the application of a single strain are gradually revealed in the process of use. For example, Trichoderma has obvious control effects on soil-borne diseases, but the inoculation agent has poor stress resistance; Bacillus has obvious control effect on leaf diseases and strong stress resistance, but the disease resistance and stress resistance of induced crops are not as good as Trichoderma. For this reason, many manufacturers at home and abroad only ferment Trichoderma and Bacillus separately, and then mix them to prepare Compound preparations, but still can not solve the problem of the convergence of Trichoderma and Bacillus products at home and abroad

Method used

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  • Biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and preparation method thereof
  • Biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and preparation method thereof
  • Biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] According to the method of the present invention, prepare the biological seed coating agent based on trichoderma-bacillus co-fermentation technology, concrete steps are as follows:

[0026] 1. Plate culture of Trichoderma and Bacillus: Inoculate Trichoderma in PDA medium and culture at 28°C for 3 days. Bacillus was inoculated in LBA medium and cultured at 30°C for 2 days.

[0027] PDA medium: 200g of potatoes were peeled and cut into pieces, steamed and boiled, the supernatant was taken, 20g of glucose, 20g of agar powder, and deionized water were added to make up to 1L, then packed into 250mL Erlenmeyer flasks, and autoclaved at 121°C for 30min.

[0028] PD medium: 200g of potatoes were peeled and cut into pieces, steamed, the supernatant was taken, 20g of glucose was added, and the volume was adjusted to 1L with deionized water, then packed into 250mL Erlenmeyer flasks, and autoclaved at 121°C for 30min.

[0029] LBA medium: Take 5g of yeast extract, 10g of sodium ch...

Embodiment 2

[0040] According to the method of the present invention, field detection trichoderma-bacillus symbiotic type biological seed coating agent growth promoting effect

[0041] Test location: Henan Zhumadian Academy of Agricultural Sciences

[0042] experiment procedure:

[0043] 1. Field test material preparation

[0044] Varieties: Jimai 22 wheat naked species, Jimai 22

[0045] Biological seed coating agent: Trichoderma-Bacillus symbiotic dry powder seed coating agent

[0046] Chemical seed coating agent: 90% thiamethoxam, 8.5% tebuconazole, 1.5% film former (control)

[0047] 2. When using chemical seed coating agent ((drug species ratio=1:200), Trichoderma-Bacillus symbiotic dry powder seed coating agent (drug species ratio=1:100)

[0048] 3. Investigate the effects of each treatment on wheat seedling emergence 20 days after planting.

[0049] 4. Compared with the blank, the symbiotic biological seed coating agent has a very significant difference in the emergence rate, a...

Embodiment 3

[0051] According to the method of the present invention, field verification Trichoderma-bacillus symbiotic dry powder seed coating agent prevents and treats corn soil-borne disease effect

[0052] Test site: Shenyang Agricultural University School Farm

[0053] experiment procedure:

[0054] 1. Field test materials:

[0055] 2. Varieties: Zhengdan 958 naked species, Zhengdan 958

[0056] 3. Biological seed coating agent: Trichoderma-Bacillus symbiotic dry powder seed coating agent; drug species ratio = 1:100

[0057] 4. Chemical seed coating agent: 90% thiamethoxam, 8.5% tebuconazole, 1.5% film-forming agent; drug species ratio = 1:200

[0058] 5. Planting method: hole sowing, 4000 plants / mu;

[0059] 6. Inoculation of pathogenic bacteria: Inoculate stem rot Fusarium graminearum and Rhizoctonia solani into the soil at 5% inoculum amount when sowing. In the middle and late July, inoculate the sheath blight with the method of inoculating bacteria-carrying grains in the lower...

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Abstract

The invention discloses a biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and a preparation method of the biological seed coating agent. According to a synthetic biology principle, a symbiotic bacterium solution is prepared from trichoderma having the characteristics of inducing disease resistance, stress resistance and the like, bacillus having the characteristics of antagonizing pathogenic bacteria, promoting crop growth and the like through a staged inoculation co-fermentation technology; and meanwhile, the symbiotic bacterium solution not only meets the balance of the contents of two types of spores, but also contains components for mutual action and specific induction of two types of microorganisms. The symbiotic bacterium liquid is mixed with diatomite and brassinolide in proportion to prepare the powder type biological seed coating agent, the biological seed coating agent contains high-content spores, antagonistic substances, growth promoting substances and the like, the growth, disease resistance and stress resistance of crops such as corn, wheat, rice and the like are obviously improved, and the technology is a new technology for beneficial bacteria treatment of food crop seeds.

Description

technical field [0001] The invention relates to a preparation technology and application of a biological seed coating agent based on the symbiotic culture (co-fermentation) of Trichoderma-Bacillus. The symbiotic bacteria liquid is mainly prepared through the co-fermentation technology, and the bacteria liquid is absorbed by diatomaceous earth, and the brassin The ester is used as an auxiliary agent to prepare a dry powder dosage type biological seed coating agent. The biological seed coating agent has a relatively high content of Trichoderma and Bacillus viable spores, antibacterial peptides, indole acetic acid, etc., so it has the characteristics of inducing crop disease resistance and promoting crop growth. Background technique [0002] Chemical seed coating agents are the most widely used agrochemical agents in the production of food crops in my country to prevent and control soil-borne diseases and underground pests, to promote crop growth, and to improve stress resistan...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20C12N3/00C12N11/14C12P21/02C12P17/10A01N63/38A01N63/22A01P3/00A01C1/06C12R1/07C12R1/885
CPCC12N1/14C12N1/20C12N3/00C12N11/14C12P21/02C12P17/10A01N63/38A01N63/22A01C1/06C12N1/205C12N1/145A01P3/00A01N63/50C12R2001/885C12R2001/125A01N43/22A01N43/38A01N61/02
Inventor 陈捷刘鹏王新华
Owner SHANGHAI JIAO TONG UNIV
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