A kind of Phalaenopsis single bud propagation method using symbiotic bacterial agent

Abstract

The invention provides a single bud propagation method of Phalaenopsis phalaenopsis, comprising the following steps: S1, taking a pedicel stem section that grows robustly in spring and full with axillary buds as explants and carries out disinfection treatment; S2, cutting the explants with axillary buds into pieces Grow 1.5cm and receive it into the bud induction medium for axillary bud induction; S3, when the axillary bud grows to 2cm in step S2, cut the sprouts and transfer them to the proliferation medium for proliferation culture; S4, when the proliferation culture sprouts > 2cm , when it grows to 3-4 leaves, remove weak seedlings and ineffective seedlings less than 2 cm, select seedlings > 2 cm to carry out rooting culture of strong seedlings; S5, move the culture bottle seedlings to 25-30 ℃ temperature conditions to strengthen seedlings for 10- After 15 days, the rooted seedlings can be taken out of the culture bottle, the residual medium attached to the roots is washed with running tap water and dried until no water drips, and then transplanted into the pre-prepared mixed matrix and pressed. If it is planted, it should be watered for the first time after planting, and then every 4-6 days after that. The method has the advantages of short reproductive cycle, strong seedlings, strong adaptability and good quality.

Description

technical field [0001] The invention relates to a single bud propagation method of Phalaenopsis using a symbiotic bacterial agent, belonging to the technical field of Phalaenopsis reproduction. Background technique [0002] Phalaenopsis has a very high ornamental value and is known as the "Queen of Orchids", which is favored by consumers at home and abroad. However, Phalaenopsis is a single stem aerial orchid, and it is difficult to carry out conventional division propagation, so most of the seedlings are propagated by tissue culture. However, the current period of tissue culture of Phalaenopsis is too long, the reproduction is too slow, and the survival rate is low, so that its output cannot meet the needs of the market. SUMMARY OF THE INVENTION [0003] The present invention provides a single bud propagation method of Phalaenopsis using a symbiotic bacterial agent, which can effectively solve the above problems. [0004] The present invention is realized in this way: ...

AI Technical Summary

Problems solved by technology

But, the cycle of the tissue culture of present Phalaenopsis is too long, and propagation is too slow, and survival rate is low, causes its output to be unable to satisfy the demand of market

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