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Protein for promoting symbiosis between plant root system and symbiotic bacteria, isolated nucleic acid molecule and application cultivation method thereof

A technology of plant roots and nucleic acid molecules, applied in the field of isolated nucleic acid molecules and proteins, to achieve the effect of providing infection efficiency, rapid and efficient symbiotic relationship

Active Publication Date: 2020-12-08
江西省农业科学院水稻研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are few reports on how to promote the rapid and efficient formation of symbiotic relationships between plants such as rice and symbiotic bacteria such as AMF.

Method used

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  • Protein for promoting symbiosis between plant root system and symbiotic bacteria, isolated nucleic acid molecule and application cultivation method thereof
  • Protein for promoting symbiosis between plant root system and symbiotic bacteria, isolated nucleic acid molecule and application cultivation method thereof
  • Protein for promoting symbiosis between plant root system and symbiotic bacteria, isolated nucleic acid molecule and application cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Gene mining to promote symbiosis between rice and mycorrhizal fungi

[0053] Dongxiang wild rice (DY) and early rice variety Zhongzao 35 (ZZ35) were used as test materials (DY was grown in Dongxiang County, Jiangxi Province, ZZ35 is an early rice variety cultivated by Chinese rice, and the seeds of the two were bred at the Rice Research Institute of Jiangxi Academy of Agricultural Sciences planted and harvested at the base;), construct the DY chromosome segment replacement line with the background of ZZ35, compare the infection phenotypes of arbuscular mycorrhiza or rhizobia, and excavate the arbuscular mycorrhizal phenotype by map-based cloning method. Genes for mycorrhizal or rhizobial infection.

[0054] AMF and rice form mycorrhizal symbiosis, and symbiosis-related marker genes are specifically expressed. Early expression genes AM1, AM3, late expression genes AM14, PT11. Extract rice root cDNA 14 days, 21 days, 28 days and 35 days after rice AMF infection, detect ...

Embodiment 2

[0066]Gene OsCERK1 that promotes the symbiosis between rice and branching mycorrhizal fungi DY functional verification of

[0067] (1) Verification gene OsCERK1 DY Features

[0068] Method: OsCERK1 DY and OsCERK1 ZH11 , linked to a 3*HA tag, was overexpressed in the ZH11 background driven by the CaMV 35S promoter. The root infection efficiency, root infection efficiency, and arbuscular abundance indicators of different transgenic lines were detected.

[0069] see results Figure 4 . Turn to OsCERK1 DY The AMF infection efficiency of the transgenic rice plants (DYa2, DY14 and DY13) including: root infection efficiency, root infection efficiency and arbuscular abundance indexes were significantly higher than those transfected with OsCERK1 ZH11 Genetic rice plants (ZH11a-6-1, ZH11a-7-3 and ZH11b-1-3), EV and ZH11. Description of OsCERK1 DY Gene and its protein compared to OsCERK1 ZH11 Genes are more able to mediate mycorrhizal signaling and enhance the symbiotic relati...

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Abstract

The invention discloses a protein for promoting symbiosis of a plant root system and symbiotic bacteria, a separated nucleic acid molecule and an application and cultivation method thereof, which relate to the technical field of plant symbiosis. Compared with SEQ ID NO.6, the amino acid sequence of the protein disclosed by the invention has at least one of the following mutations: I118T and S121T.The protein can enable the plant root system to be more easily infected by symbiotic bacteria, is high in infection efficiency, and can quickly and efficiently establish a symbiotic relationship withthe symbiotic bacteria. The protein can be used for cultivating a plant variety which is easy to form a symbiotic relationship with the symbiotic bacteria.

Description

technical field [0001] The invention relates to the technical field of plant symbiosis, in particular to a protein for promoting the symbiosis of plant roots and symbiotic bacteria, an isolated nucleic acid molecule and a cultivation method thereof. Background technique [0002] The current global population is 7 billion and continues to grow rapidly. It is estimated that the global population will reach 9.5 billion by 2050. To meet such population growth, modern agriculture must significantly increase crop yields. Rice is one of the most important food crops, serving as a staple food for more than 3.5 billion people worldwide. The International Rice Research Institute (IRRI) estimates that for each of the next 20 years, an additional 8-10-fold increase in annual rice production will be required (IRRI 2015). A large number of pathogenic microorganisms endanger the production of rice. IRRI statistics show that the average annual rice yield is reduced by 37% due to the impa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8213C12N15/8251
Inventor 黄仁良沈显华
Owner 江西省农业科学院水稻研究所
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