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Method for reducing cadmium content of overground part of paddy rice

A technology of rice and cadmium, which is applied in the field of utilization of microbial resources and agricultural product quality and safety, can solve the problems of popularization and application, arbuscular mycorrhizal fungi cannot be artificially cultivated, etc., to promote rice growth, reduce heavy metal cadmium residues, and grow robustly Effect

Inactive Publication Date: 2014-06-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, arbuscular mycorrhizal fungi cannot be cultivated artificially, and their popularization and application in production have been affected.

Method used

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  • Method for reducing cadmium content of overground part of paddy rice
  • Method for reducing cadmium content of overground part of paddy rice
  • Method for reducing cadmium content of overground part of paddy rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for reducing cadmium accumulation in rice shoots, comprising the steps of:

[0037] Activation of strains: Inoculate the Pyromorpha indica strains stored in the refrigerator on Kafer medium (KM) plates, and culture them in a 28°C incubator in the dark for 7 days. Use a hole punch with a diameter of 5 mm to punch holes at the edge of the activated Pyrocytosporum indica colony.

[0038]Bacteria culture: Prepare liquid Kafer medium and liquid Kafer medium containing nanomaterial ZnO(30nm) 0.01g / L (i.e. replace ZnSO4 with nanomaterial ZnO(30nm)), pack in 250ml triangular conical flasks, each Bottle 150ml of culture medium and sterilize it for later use. Under sterile conditions, add the mycelia blocks of Pyromorpha indica into the Erlenmeyer flask containing the above medium, 4 pieces of mycelium blocks per bottle; place in a constant temperature shaker at 120rpm, 28°C, avoid light and shake for 5 days .

[0039] Collection of mycelium: filter with four layers o...

Embodiment 2

[0045] Change the potted rice in Example 1 into hydroponic rice, cultivate it with nutrient solution (refer to the recipe of the International Rice Research Institute (IRRI)), and place it in a plant growth room with (25±1)°C and 14h / 10h alternating light and dark. Replacing the nutrient solution every 5 days can also reduce the accumulation of cadmium in the aboveground part of rice. For example, after 12 days of 3 mg / L cadmium stress treatment, the cadmium content in the shoots of rice inoculated with Pyromorpha indica decreased by 38.4% compared with the control ( image 3 ).

[0046] Under hydroponic conditions, P. indica significantly improved the tolerance of rice to cadmium stress. For example, on the 12th day after 25mg / L cadmium was processed, the old leaves of the rice seedlings inoculated with Pyromorpha indica began to show slight yellowing symptoms, while the maximum functional leaves of the rice seedlings of the control group had lost chlorosis; while at 50mg / L ...

Embodiment 3

[0052] In Example 1, the mycelia block of Pyriformis indica was changed into Pyromorpha indica spore liquid, inoculated in the liquid Kafer medium containing 0.01g / LZnO, and cultured in a constant temperature shaker at 100rpm and 25°C in the dark for 7 days. The rest are the same as Example 1, which can finally reduce the accumulation of cadmium in the aboveground part of rice.

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Abstract

The invention provides a method for reducing cadmium content of an overground part of paddy rice. The method comprises the following specific steps: (1) by use of utilizing nano material ZnO to replace ZnSO4.7H2O in an original Kafer culture medium, adding 10mg of nano material ZnO into per litre of the culture medium; (2) inoculating the activated piriformospora indica into the liquid Kafer culture medium obtained in the step (1) for culturing, then collecting mycelia, preparing a piriformospora indica mycelium solution, then pouring the piriformospora indica mycelium solution to the root of a paddy rice seedling, and performing root-irrigation treatment so as to colonize the piriformospora indica at the root of the paddy rice seedling; (3) after performing piriformospora indica colonization, transplanting the paddy rice into a large field, and then performing routine water and fertilizer management so as to enable the paddy rice to grow normally. The method is easy to master, relatively low in cost, simple and practical, environment-friendly, incapable of causing secondary pollution, applicable to production of chemical-free rice, not only applicable to planting of rice in heavy metal contaminated soil, and also applicable to uncontaminated soil.

Description

technical field [0001] The invention relates to the technical field of the utilization of microbial resources and the quality and safety of agricultural products, in particular to a method for reducing the cadmium content in the aboveground part of rice. Background technique [0002] With the extensive use of chemical fertilizers and pesticides and the rapid development of industry, environmental pollution has become increasingly serious. Cadmium is a toxic heavy metal and one of the major environmental pollutants. Moderate levels of cadmium-contaminated plow layer soils will not cause toxic effects on plants, but can make the edible parts of crops accumulate a considerable amount of cadmium, which endangers human health through the food chain. At present, the pollution of rice fields by cadmium and the accumulation of cadmium in rice have become serious agricultural problems. Therefore, how to reduce cadmium pollution in paddy fields and cadmium accumulation in grains has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G1/04A01G7/06A01G22/22
Inventor 楼兵干吴金丹刘晓曦陈乾高其康林福呈
Owner ZHEJIANG UNIV
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