A method for combined control of tobacco bacterial wilt by utilizing Pyriformis indica and trichloroisocyanuric acid
A technology of trichloroisocyanuric acid and piriformis indica, applied in the field of joint prevention and treatment of tobacco bacterial wilt by using piriformis indica and trichloroisocyanuric acid, can solve the problems that anti-bacteria and pesticides cannot be used together, and achieve improvement The effect of prevention and control, the effect of consolidating the prevention of diseases, and reducing the occurrence of bacterial wilt
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[0041] The present invention will be further described below in conjunction with specific examples.
[0042]Materials used in the present invention: Indian piriformis (P.india) was donated by Professor Ajit Verma of the University of Edinburgh in India; Gift from researcher Feng Jie; China Tobacco 100 seeds provided by Zhengzhou Tobacco Research Institute;
[0043] 42% trichloroisocyanuric acid was purchased from Hunan Marine Biological Engineering Co., Ltd.
[0044] 1 Plate Determination of Bacteriostatic Effect on Ralstonia solanacearum
[0045] 1.1 Bacterial suspension preparation
[0046] The strong pathogenic strain RS-TB3 preserved in sterile water was placed on a TTC plate (TTC medium formula: beef extract 3.0g, yeast extract 1g, peptone 5g, glucose 10g, agar 20g, distilled water 1000mL, pH 6.8-7.2. Sterilize at 121°C for 15 minutes, cool to 50°C, add triphenyl tetraazolium chloride salt to make the final concentration reach 50 mg / L) and draw a line on it. After cult...
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